Time-resolved ultraviolet resonance Raman studies of protein structure: application to bacteriorhodopsin

Biochemistry ◽  
1992 ◽  
Vol 31 (23) ◽  
pp. 5328-5334 ◽  
Author(s):  
James B. Ames ◽  
Marianne Ros ◽  
Jan Raap ◽  
Johan Lugtenburg ◽  
Richard A. Mathies
Keyword(s):  
Protein Structure ◽  
Resonance Raman ◽  
Time Resolved ◽  
Ultraviolet Resonance Raman

scholarly journals Time-Resolved Ultraviolet Resonance Raman of Protein Structural Changes in The KI-Intermediate Of Bacteriorhodopsin

1999 ◽  
Vol 19 (1-4) ◽  
pp. 165-168 ◽  
Author(s):  
Shoji Kaminaka ◽  
Richard A. Mathies
Keyword(s):  
Structural Changes ◽  
Resonance Raman ◽  
Difference Spectrum ◽  
Binding Pocket ◽  
Stray Light ◽  
Time Resolved ◽  
Ultraviolet Resonance Raman ◽  
Transient Intermediates ◽  
Retinal Binding Pocket ◽  
Low Dispersion

To obtain high quality time-resolved ultraviolet resonance Raman (UVRR) spectra of transient intermediates in the bacteriorhodopsin (BR) photocycle, we developed a new UVRR spectrometer. A home-made F=3.5 prism prefilter was used in front of a 50 cm CCD detected spectrograph to give high throughput, wide tunability, and excellent stray light rejection along with low dispersion. Using this system, we obtained 239.5 nm excited time-resolved UVRR spectra of BR which revealed small but significant features associated with the formation of the KL-intermediate at 10 ns delays. This difference spectrum exhibits intensity decreases at 1624, 1561, 1012 and 763cm-1 due to an altered environment of one or more Trp residues and a frequency shift of the Tyr ʋ8b band at 1602 cm-1. These signals show that the photoisomerization of retinal from all-trans to 13-cis induces significant changes in the structure and environment of aromatic residues that line the retinal binding pocket in only 10 ns.

scholarly journals Ultraviolet resonance Raman spectroscopic markers for protein structure and dynamics

2018 ◽  
Vol 103 ◽  
pp. 223-229 ◽  
Author(s):  
Ryan S. Jakubek ◽  
Joseph Handen ◽  
Stephen E. White ◽  
Sanford A. Asher ◽  
Igor K. Lednev
Keyword(s):  
Protein Structure ◽  
Resonance Raman ◽  
Raman Spectroscopic ◽  
Structure And Dynamics ◽  
Ultraviolet Resonance Raman ◽  
Protein Structure And Dynamics
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