Probing the regulatory site of Escherichia coli aspartate transcarbamoylase by site-specific mutagenesis

Yang Zhang; Evan R. Kantrowitz

doi:10.1021/bi00118a022

Probing the regulatory site of Escherichia coli aspartate transcarbamoylase by site-specific mutagenesis

Biochemistry ◽

10.1021/bi00118a022 ◽

1992 ◽

Vol 31 (3) ◽

pp. 792-798 ◽

Cited By ~ 18

Author(s):

Yang Zhang ◽

Evan R. Kantrowitz

Keyword(s):

Escherichia Coli ◽

Aspartate Transcarbamoylase ◽

Regulatory Site ◽

Site Specific

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Arginine 54 in the active site of escherichia coli aspartate transcarbamoylase is critical for catalysis: A site-specific mutagenesis, NMR, and X-ray crystallographic study

Protein Science ◽

10.1002/pro.5560011105 ◽

1992 ◽

Vol 1 (11) ◽

pp. 1435-1446 ◽

Cited By ~ 18

Author(s):

Jeffrey W. Stebbins ◽

Diane E. Robertson ◽

Mary F. Roberts ◽

Raymond C. Stevens ◽

William N. Lipscomb ◽

...

Keyword(s):

Escherichia Coli ◽

Active Site ◽

Aspartate Transcarbamoylase ◽

Site Specific ◽

X Ray ◽

Crystallographic Study ◽

A Site

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Site-directed mutagenesis of a residue located in the regulatory site of Escherichia coli aspartate transcarbamoylase. Involvement of lysine 94 in effector binding and the allosteric mechanism.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)57303-3 ◽

1988 ◽

Vol 263 (3) ◽

pp. 1320-1324

Author(s):

Y Zhang ◽

M M Ladjimi ◽

E R Kantrowitz

Keyword(s):

Escherichia Coli ◽

Site Directed Mutagenesis ◽

Aspartate Transcarbamoylase ◽

Directed Mutagenesis ◽

Regulatory Site ◽

Allosteric Mechanism ◽

Effector Binding

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Faculty Opinions recommendation of Time evolution of the quaternary structure of Escherichia coli aspartate transcarbamoylase upon reaction with the natural substrates and a slow, tight-binding inhibitor.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.12818969.14100069 ◽

2011 ◽

Author(s):

Jean-Pierre Changeux

Keyword(s):

Escherichia Coli ◽

Time Evolution ◽

Quaternary Structure ◽

Tight Binding ◽

Aspartate Transcarbamoylase

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Crystallization and preliminary X-ray diffraction studies of an inactive mutant aspartate transcarbamoylase from Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)69302-6 ◽

1981 ◽

Vol 256 (10) ◽

pp. 4691-4692

Author(s):

R. Kim ◽

T.S. Young ◽

H.K. Schachman ◽

S.H. Kim

Keyword(s):

Escherichia Coli ◽

Aspartate Transcarbamoylase ◽

X Ray Diffraction ◽

X Ray

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Calorimetric estimate of the enthalpy change for the substrate-promoted conformational transition of aspartate transcarbamoylase from Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)69358-0 ◽

1981 ◽

Vol 256 (10) ◽

pp. 5005-5015

Author(s):

A. Shrake ◽

A. Ginsburg ◽

H.K. Schachman

Keyword(s):

Escherichia Coli ◽

Conformational Transition ◽

Enthalpy Change ◽

Aspartate Transcarbamoylase

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Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)37520-8 ◽

1994 ◽

Vol 269 (8) ◽

pp. 5720-5724

Author(s):

I. Yamato ◽

M. Kotani ◽

Y. Oka ◽

Y. Anraku

Keyword(s):

Escherichia Coli ◽

Amino Acid ◽

Amino Acid Residue ◽

Site Specific ◽

Charged Amino Acid Residue ◽

Specific Alteration ◽

Membrane Spanning ◽

Positively Charged

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Site-specific alteration of cysteine 176 and cysteine 234 in the lactose carrier of Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)67309-0 ◽

1986 ◽

Vol 261 (25) ◽

pp. 11765-11769

Author(s):

R J Brooker ◽

T H Wilson

Keyword(s):

Escherichia Coli ◽

Site Specific ◽

Specific Alteration

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Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases.

Journal of Bacteriology ◽

10.1128/jb.169.8.3840-3843.1987 ◽

1987 ◽

Vol 169 (8) ◽

pp. 3840-3843 ◽

Cited By ~ 112

Author(s):

C J Dorman ◽

C F Higgins

Keyword(s):

Escherichia Coli ◽

Phase Variation ◽

Integration Host Factor ◽

Host Factor ◽

Site Specific

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Design of a membrane protein for site-specific proteolysis: properties of engineered factor Xa protease sites in the lactose permease of Escherichia coli

Biochemistry ◽

10.1021/bi00004a001 ◽

1995 ◽

Vol 34 (4) ◽

pp. 1107-1112 ◽

Cited By ~ 21

Author(s):

Miklos Sahin-Toth ◽

Rhonda L. Dunten ◽

H. Ronald Kaback

Keyword(s):

Escherichia Coli ◽

Membrane Protein ◽

Factor Xa ◽

Lactose Permease ◽

Site Specific

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Proteolytic Cleavage of the Multienzyme Polypeptide CAD to Release the Mammalian Aspartate Transcarbamoylase. Biochemical Comparison with the Homologous Escherichia Coli Catalytic Subunit

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1994.0845b.x ◽

1994 ◽

Vol 225 (3) ◽

pp. 845-853 ◽

Cited By ~ 7

Author(s):

Benjamin Hemmens ◽

Elizabeth A. Carrey

Keyword(s):

Escherichia Coli ◽

Proteolytic Cleavage ◽

Catalytic Subunit ◽

Aspartate Transcarbamoylase ◽

Biochemical Comparison

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