scholarly journals Interaction of theEscherichia coliReplication Terminator Protein (Tus) with DNA:  A Model Derived from DNA-Binding Studies of Mutant Proteins by Surface Plasmon Resonance†

Biochemistry ◽  
2000 ◽  
Vol 39 (39) ◽  
pp. 11989-11999 ◽  
Author(s):  
Cameron Neylon ◽  
Susan E. Brown ◽  
Andrew V. Kralicek ◽  
Caroline S. Miles ◽  
Christopher A. Love ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Dna Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Studies ◽  
Mutant Proteins ◽  
Dna Binding Studies

Localized and Propagating Surface Plasmon Resonance Sensors: A Study Using Carbohydrate Binding Protein

2005 ◽  
Vol 876 ◽  
Author(s):  
Chanda Yonzon ◽  
Richard P. Van Duyne
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Binding ◽  
Gold Surface ◽  
Optical Biosensor ◽  
Localized Surface Plasmon ◽  
Carbohydrate Binding ◽  
Binding Studies ◽  
Assembled Monolayers

AbstractThis work encompasses a comparative analysis of the properties of two optical biosensor platforms: (1) the propagating surface plasmon resonance (SPR) sensor based on a planar, thin film gold surface and (2) the localized surface plasmon resonance (LSPR) sensor based on surface confined Ag nanoparticles fabricated by nanosphere lithography. The binding of Concanavalin A (ConA) to mannose-functionalized self-assembled monolayers (SAMs) is chosen to illustrate the similarities and the differences of these sensors. A comprehensive set of non-specific binding studies demonstrate that the single transduction mechanism is due to the specific binding of ConA to the mannose-functionalized surface. Finally, an elementary (2x1) multiplexed version of a LSPR carbohydrate sensing chip to probe the simultaneous binding of ConA to mannose and galactose-functionalized SAMs is also demonstrated.

scholarly journals Differential binding studies applying functional protein microarrays and surface plasmon resonance

PROTEOMICS ◽  
2006 ◽  
Vol 6 (19) ◽  
pp. 5132-5139 ◽  
Author(s):  
Harald Seitz ◽  
Silke Hutschenreiter ◽  
Claus Hultschig ◽  
Carsten Zeilinger ◽  
Bastian Zimmermann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Microarrays ◽  
Binding Studies ◽  
Functional Protein ◽  
Differential Binding

scholarly journals Insights into the DNA-binding mechanism of a LytTR-type transcription regulator

2016 ◽  
Vol 36 (2) ◽  
Author(s):  
Stefan Behr ◽  
Ralf Heermann ◽  
Kirsten Jung
Keyword(s):  
Surface Plasmon Resonance ◽  
Dna Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Response Regulator ◽  
Binding Mechanism ◽  
Transcription Regulator ◽  
Interaction Map ◽  
Promoter Dna ◽  
Type Response

A combination of surface plasmon resonance (SPR) spectroscopy and interaction map® (IM) analysis was used to characterize binding of the LytTR-type response regulator YpdB to promoter DNA. YpdB follows an ‘AB-BA’ mechanism involving sequential and cooperative DNA binding followed by rapid successive promoter clearance.

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