Binding of phenol and analogs to alanine complexes of tyrosine phenol-lyase from Citrobacter freundii: Implications for the mechanisms of .alpha.,.beta.-elimination and alanine racemization

Biochemistry ◽  
1993 ◽  
Vol 32 (43) ◽  
pp. 11591-11599 ◽  
Author(s):  
Haoyuan Chen ◽  
Robert S. Phillips
Keyword(s):  
Citrobacter Freundii ◽  
Tyrosine Phenol Lyase ◽  
Beta Elimination ◽  
Alpha Beta

scholarly journals Threonine-124 and phenylalanine-448 in Citrobacter freundii tyrosine phenol-lyase are necessary for activity with l-tyrosine

2002 ◽  
Vol 363 (3) ◽  
pp. 745 ◽  
Author(s):  
Tatyana V. DEMIDKINA ◽  
Maria V. BARBOLINA ◽  
Nicolai G. FALEEV ◽  
Bakthavatsalam SUNDARARAJU ◽  
Paul D. GOLLNICK ◽  
...  
Keyword(s):  
Citrobacter Freundii ◽  
Tyrosine Phenol Lyase

scholarly journals Rapid and selective modification of phosphoserine residues catalysed by Ba2+ ions for their detection during peptide microsequencing

1991 ◽  
Vol 280 (1) ◽  
pp. 261-265 ◽  
Author(s):  
M F Byford
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Metal Ions ◽  
Ion Concentration ◽  
Thiol Groups ◽  
Disulphide Bonds ◽  
Rate Of Reaction ◽  
Alkaline Conditions ◽  
Beta Elimination ◽  
Alpha Beta

The beta-elimination of phosphoserine residues by dilute alkali is catalysed by the presence of group II metal ions. The use of 0.1 M-Ba (OH)2 catalysed the rate of beta-elimination of phosphoserine by more than two orders of magnitude compared with the use of NaOH at the same OH-ion concentration. Serine and threonine residues are unaffected by this treatment. Free thiol groups and disulphide bonds are labile to these conditions, but carboxymethylcysteine is stable. The rate of beta-elimination of O-glycosidically linked moieties is not catalysed under these conditions, and the rate of reaction is thus two orders of magnitude slower than for phosphoserine. This specific catalysis was readily exploited in the rapid and selective modification of phosphoserine residues under mildly alkaline conditions with the nucleophile methylamine via the alpha beta-desaturated dehydroalanine intermediate to yield the beta-methylaminoalanine residue. This modified residue could be easily detected on sequence analysis and in amino acid compositions.

.alpha.'.beta. Elimination of carbanions derived from thioethers

1979 ◽  
Vol 101 (12) ◽  
pp. 3283-3288 ◽  
Author(s):  
Jean Francois Biellmann ◽  
Hugues d'Orchymont ◽  
Jean Louis Schmitt
Keyword(s):  
Beta Elimination ◽  
Alpha Beta

scholarly journals Purification and characterization of human hepatic cysteine-conjugate β-lyase

1986 ◽  
Vol 235 (2) ◽  
pp. 569-575 ◽  
Author(s):  
H Tomisawa ◽  
S Ichihara ◽  
H Fukazawa ◽  
N Ichimoto ◽  
M Tateishi ◽  
...  
Keyword(s):  
Human Liver ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Elimination Reaction ◽  
Purification And Characterization ◽  
Ph Optimum ◽  
Beta Elimination ◽  
Alpha Beta ◽  
Hydroxyapatite Gel

Cysteine-conjugate beta-lyase (EC 4.4.1.13) was purified about 880-fold from human liver obtained post mortem. The purification procedure included (NH4)2SO4 precipitation, chromatography on DEAE-cellulose and hydroxyapatite, gel filtration on Sephadex G-200, and chromatofocusing. The purified enzyme cleaves the C-S bond of several S-aryl-L-cysteines to yield equimolar amounts of thiols, pyruvic acid and ammonia via an alpha beta-elimination reaction. The Mr of the enzyme was estimated to be 88,000 by gel filtration. The enzyme is thermolabile, has a pH optimum of 8.5, and an apparent Km of 0.7 mM towards S-(p-bromophenyl)-L-cysteine. The enzyme requires pyridoxal 5′-phosphate as a cofactor, and hence the enzyme activity was completely abolished by hydroxylamine. No effect of EDTA or thiol-blocking reagents was observed on the activity of the enzyme.

Isolation of the tyrosine phenol lyase gene from Citrobacter freundii

1990 ◽  
Vol 12 (11) ◽  
pp. 805-810 ◽  
Author(s):  
Jacek Polak ◽  
Henry Brzeski
Keyword(s):  
Citrobacter Freundii ◽  
Tyrosine Phenol Lyase
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