Functional independence of the kringle 4 and kringle 5 regions of human plasminogen

Biochemistry ◽  
1993 ◽  
Vol 32 (34) ◽  
pp. 8799-8806 ◽  
Author(s):  
Nick Menhart ◽  
Stephen G. McCance ◽  
Louis C. Sehl ◽  
Francis J. Castellino
Keyword(s):  
Functional Independence ◽  
Kringle 5 ◽  
Human Plasminogen

159 Structure and ligand binding properties of the kringle 5 domain of human plasminogen

1997 ◽  
Vol 11 ◽  
pp. 45 ◽  
Author(s):  
F.J. Castellino ◽  
S.G. McCance ◽  
Y. Chang ◽  
I. Mochalkin ◽  
A. Tulinsky
Keyword(s):  
Ligand Binding ◽  
Binding Properties ◽  
Kringle 5 ◽  
Human Plasminogen

Erratum: Adeno-associated virus-mediated delivery of kringle 5 of human plasminogen inhibits orthotopic growth of ovarian cancer

Gene Therapy ◽  
2010 ◽  
Vol 17 (7) ◽  
pp. 938-938
Author(s):  
T M Bui Nguyen ◽  
I V Subramanian ◽  
X Xiao ◽  
P Nguyen ◽  
S Ramakrishnan
Keyword(s):  
Ovarian Cancer ◽  
Adeno Associated Virus ◽  
Kringle 5 ◽  
Human Plasminogen

scholarly journals Modification of Cyclic NGR Tumor Neovasculature-Homing Motif Sequence to Human Plasminogen Kringle 5 Improves Inhibition of Tumor Growth

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e37132 ◽  
Author(s):  
Weiwei Jiang ◽  
Guanghui Jin ◽  
Dingyuan Ma ◽  
Feng Wang ◽  
Tong Fu ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Kringle 5 ◽  
Inhibition Of Tumor Growth ◽  
Human Plasminogen ◽  
Tumor Neovasculature

Kringle 5 of human plasminogen, an angiogenesis inhibitor, induces both autophagy and apoptotic death in endothelial cells

Blood ◽  
2007 ◽  
Vol 109 (11) ◽  
pp. 4793-4802 ◽  
Author(s):  
Tri Minh Bui Nguyen ◽  
Indira V. Subramanian ◽  
Ameeta Kelekar ◽  
Sundaram Ramakrishnan
Keyword(s):  
Endothelial Cells ◽  
Prolonged Exposure ◽  
Angiogenesis Inhibitor ◽  
Cancer Therapeutics ◽  
Beclin 1 ◽  
Endothelial Cell Proliferation ◽  
Kringle 5 ◽  
Mitochondrial Membrane Depolarization ◽  
Human Plasminogen ◽  
Induced Apoptosis

Abstract Inhibition of endothelial cell proliferation and angiogenesis is emerging as an important strategy in cancer therapeutics. Kringle 5 (K5) of human plasminogen is a potent angiogenesis inhibitor. Previous studies have shown K5 exposure promotes caspase activity and apoptosis in endothelial cells. Here we report that K5 treatment evokes an autophagic response in endothelial cells that is specific and initiated even in the absence of nutritional stress. Endothelial cells exposed to K5 up-regulated Beclin 1 levels within a few hours. Furthermore, progressively increasing amounts of antiapoptotic Bcl-2 were found to be complexed with Beclin 1, although total levels of Bcl-2 remained unchanged. Prolonged exposure to K5 ultimately led to apoptosis via mitochondrial membrane depolarization and caspase activation in endothelial cells. Knocking down Beclin 1 levels by RNA interference decreased K5 induced autophagy but accelerated K5-induced apoptosis. These studies suggest that interfering with the autophagic survival response can potentiate the antiangiogenic effects of Kringle 5 in endothelial cells.

Sign in / Sign up

Export Citation Format

Share Document