Minimum intron requirements for tRNA splicing and nuclear transport in Xenopus oocytes

Biochemistry ◽  
1993 ◽  
Vol 32 (33) ◽  
pp. 8575-8581 ◽  
Author(s):  
Rosalind C. Haselbeck ◽  
Chris L. Greer
Keyword(s):  
Xenopus Oocytes ◽  
Nuclear Transport ◽  
Trna Splicing

Signals and receptors for the nuclear transport of TFIIIA in Xenopus oocytes

2004 ◽  
Vol 83 (2) ◽  
pp. 55-66 ◽  
Author(s):  
Jörg Wischnewski ◽  
Falko Rudt ◽  
Tomas Pieler
Keyword(s):  
Xenopus Oocytes ◽  
Nuclear Transport

scholarly journals Structural requirements of 5S rRNA for nuclear transport, 7S ribonucleoprotein particle assembly, and 60S ribosomal subunit assembly in Xenopus oocytes.

1993 ◽  
Vol 13 (11) ◽  
pp. 6819-6831 ◽  
Author(s):  
L A Allison ◽  
M T North ◽  
K J Murdoch ◽  
P J Romaniuk ◽  
S Deschamps ◽  
...  
Keyword(s):  
Xenopus Oocytes ◽  
Nuclear Transport ◽  
Atp Depletion ◽  
5S Rrna ◽  
Nuclear Pore ◽  
Particle Assembly ◽  
Structural Requirements ◽  
Essential Information ◽  
Ribosomal Subunits ◽  
Subunit Assembly

Structural requirements of 5S rRNA for nuclear transport and RNA-protein interactions have been studied by analyzing the behavior of oocyte-type 5S rRNA and of 31 different in vitro-generated mutant transcripts after microinjection into the cytoplasm of Xenopus oocytes. Experiments reveal that the sequence and secondary and/or tertiary structure requirements of 5S rRNA for nuclear transport, storage in the cytoplasm as 7S ribonucleoprotein particles, and assembly into 60S ribosomal subunits are complex and nonidentical. Elements of loops A, C, and E, helices II and V, and bulged and hinge nucleotides in the central domain of 5S rRNA carry the essential information for these functional activities. Assembly of microinjected 5S rRNA into 60S ribosomal subunits was shown to occur in the nucleus; thus, the first requirement for subunit assembly is nuclear targeting. The inhibitory effects of ATP depletion, wheat germ agglutinin, and chilling on the nuclear import of 5S rRNA indicate that it crosses the nuclear envelope through the nuclear pore complex by a pathway similar to that used by karyophilic proteins.

Structural requirements of 5S rRNA for nuclear transport, 7S ribonucleoprotein particle assembly, and 60S ribosomal subunit assembly in Xenopus oocytes

1993 ◽  
Vol 13 (11) ◽  
pp. 6819-6831
Author(s):  
L A Allison ◽  
M T North ◽  
K J Murdoch ◽  
P J Romaniuk ◽  
S Deschamps ◽  
...  
Keyword(s):  
Xenopus Oocytes ◽  
Nuclear Transport ◽  
Atp Depletion ◽  
5S Rrna ◽  
Nuclear Pore ◽  
Particle Assembly ◽  
Structural Requirements ◽  
Essential Information ◽  
Ribosomal Subunits ◽  
Subunit Assembly

Structural requirements of 5S rRNA for nuclear transport and RNA-protein interactions have been studied by analyzing the behavior of oocyte-type 5S rRNA and of 31 different in vitro-generated mutant transcripts after microinjection into the cytoplasm of Xenopus oocytes. Experiments reveal that the sequence and secondary and/or tertiary structure requirements of 5S rRNA for nuclear transport, storage in the cytoplasm as 7S ribonucleoprotein particles, and assembly into 60S ribosomal subunits are complex and nonidentical. Elements of loops A, C, and E, helices II and V, and bulged and hinge nucleotides in the central domain of 5S rRNA carry the essential information for these functional activities. Assembly of microinjected 5S rRNA into 60S ribosomal subunits was shown to occur in the nucleus; thus, the first requirement for subunit assembly is nuclear targeting. The inhibitory effects of ATP depletion, wheat germ agglutinin, and chilling on the nuclear import of 5S rRNA indicate that it crosses the nuclear envelope through the nuclear pore complex by a pathway similar to that used by karyophilic proteins.

scholarly journals The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.

1990 ◽  
Vol 111 (2) ◽  
pp. 309-321 ◽  
Author(s):  
A K Hopper ◽  
H M Traglia ◽  
R W Dunst
Keyword(s):  
Gene Product ◽  
Indirect Immunofluorescence ◽  
Rna Processing ◽  
Nuclear Transport ◽  
Stress Conditions ◽  
Nuclear Proteins ◽  
Intracellular Location ◽  
Trna Splicing ◽  
Immunofluorescence Localization ◽  
Endogenous Protein

The yeast RNA1 gene is required for RNA processing and nuclear transport of RNA. The rna1-1 mutation of this locus causes defects in pre-tRNA splicing, processing of the primary pre-rRNA transcript, production of mRNA and export of RNA from the nucleus to the cytosol. To understand how this gene product can pleiotropically affect these processes, we sought to determine the intracellular location of the RNA1 protein. As determined by indirect immunofluorescence localization and organelle fractionation, the RNA1 antigen is found exclusively or primarily in the cytoplasm. Only a tiny fraction of the endogenous protein could be localized to and functional in the nucleus. Furthermore, the RNA1 antigen does not localize differently under stress conditions. These findings suggest that the RNA1 protein is not directly involved in RNA processing but may modify nuclear proteins or otherwise transmit a signal from the cytosol to the nucleus or play a role in maintaining the integrity of the nucleus.

Nuclear transport: never-ending cycles of signals and receptors

2000 ◽  
Vol 36 ◽  
pp. 89-103 ◽  
Author(s):  
Dianne M. Barry ◽  
Susan R. Wente
Keyword(s):  
Nuclear Transport
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