Binding of transcription factor Sp1 to GC box DNA revealed by footprinting analysis: Different contact of three zinc fingers and sequence recognition mode

Biochemistry ◽  
1993 ◽  
Vol 32 (23) ◽  
pp. 5994-6001 ◽  
Author(s):  
Jun Kuwahara ◽  
Atsuo Yonezawa ◽  
Mayumi Futamura ◽  
Yukio Sugiura
Keyword(s):  
Transcription Factor ◽  
Zinc Fingers ◽  
Sequence Recognition ◽  
Transcription Factor Sp1 ◽  
Gc Box

scholarly journals Nuclear receptors RXRα:RARα are repressors for human MRP3 expression

2007 ◽  
Vol 292 (5) ◽  
pp. G1221-G1227 ◽  
Author(s):  
Wensheng Chen ◽  
Shi-Ying Cai ◽  
Shuhua Xu ◽  
Lee A. Denson ◽  
Carol J. Soroka ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Promoter Activity ◽  
Regulatory Elements ◽  
Luciferase Reporter ◽  
Dependent Manner ◽  
Site Mutation ◽  
Transcription Factor Sp1 ◽  
Gc Box ◽  
Rna Knockdown ◽  
Interfering Rna

Multidrug resistance-associated protein MRP3/Mrp3 (ABCC3) is upregulated in cholestasis, an adaptive response that may protect the liver from accumulation of toxic compounds, such as bile salts and bilirubin conjugates. However, the mechanism of this upregulation is poorly understood. We and others have previously reported that fetoprotein transcription factor/liver receptor homolog-1 is an activator of MRP3/Mrp3 expression. In searching for additional regulatory elements in the human MRP3 promoter, we have now identified nuclear receptor retinoic X receptor-α:retinoic acid receptor-α (RXRα:RARα) as a repressor of MRP3 activation by transcription factor Sp1. A luciferase reporter assay demonstrated that cotransfection of transcription factor Sp1 stimulates the MRP3 promoter activity and that additions of RXRα:RARα abrogated this activation in a dose-dependent manner. Site mutations and gel shift assays have identified a Sp1 binding GC box motif at −113 to −108 nts upstream from the MRP3 translation start site, where RXRα:RARα specifically reduced Sp1 binding to this site. Mutation of the GC box also reduced MRP3 promoter activity. The functional role of RXRα:RARα as a repressor of MRP3 expression was further confirmed by RARα small-interfering RNA knockdown in HepG2 cells, which upregulated endogenous MRP3 expression. In summary, our results indicate that activator Sp1 and repressor RXRα:RARα act in concert to regulate MRP3 expression. Since RXRα:RARα expression is diminished by cholestatic liver injury, loss of RXRα:RARα may lead to upregulation of MRP3/Mrp3 expression in these disorders.

Effects of 8-oxo-7,8-dihydro-2′-deoxyguanosine on the binding of the transcription factor Sp1 to its cognate target DNA sequence (GC box)

1999 ◽  
Vol 31 (3) ◽  
pp. 217-229 ◽  
Author(s):  
Olivier Ramon ◽  
Sylvie Sauvaigo ◽  
Didier Gasparutto ◽  
Patrice Faure ◽  
Alain Favier ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Sequence ◽  
Transcription Factor Sp1 ◽  
Target Dna ◽  
Gc Box

Tuning the reactivity of Sp1 zinc fingers with platinum complexes

2016 ◽  
Vol 45 (21) ◽  
pp. 8712-8716 ◽  
Author(s):  
Zhifeng Du ◽  
Raphael E. F. de Paiva ◽  
Yun Qu ◽  
Nicholas Farrell
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Lewis Acid ◽  
Zinc Fingers ◽  
Platinum Complexes ◽  
Coordination Unit ◽  
Transcription Factor Sp1 ◽  
Dinuclear Platinum

The microenvironment around a zinc finger coordination unit affects the reactivity of apparently similar ZFs with Lewis acid platinum electrophiles. The unique dicysteine-bridged dinuclear platinum unit is the product of the reaction of [PtCl2(en)] and the F3 of the transcription factor Sp1.

Interference of benzo[a]pyrene diol epoxide-deoxyguanosine adducts in a GC box with binding of the transcription factor Sp1

1996 ◽  
Vol 16 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Michael C. MacLeod ◽  
K. Leslie Powell ◽  
Vladimir A. Kuzmin ◽  
Aleksandr Kolbanovskiy ◽  
Nicholas E. Geacintov
Keyword(s):  
Transcription Factor ◽  
Diol Epoxide ◽  
Transcription Factor Sp1 ◽  
Gc Box

scholarly journals Inhibitory Effects of Heavy Metals on Transcription Factor Sp1.

2000 ◽  
Vol 38 (2) ◽  
pp. 224-227 ◽  
Author(s):  
Pengfei GONG ◽  
Yasumitsu OGRA ◽  
Shinji KOIZUMI
Keyword(s):  
Heavy Metals ◽  
Transcription Factor ◽  
Inhibitory Effects ◽  
Transcription Factor Sp1

NMR Structure of Transcription Factor Sp1 DNA Binding Domain†,‡

Biochemistry ◽  
2004 ◽  
Vol 43 (51) ◽  
pp. 16027-16035 ◽  
Author(s):  
Shinichiro Oka ◽  
Yasuhisa Shiraishi ◽  
Takuya Yoshida ◽  
Tadayasu Ohkubo ◽  
Yukio Sugiura ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Domain ◽  
Nmr Structure ◽  
Dna Binding Domain ◽  
Transcription Factor Sp1

scholarly journals Loss of polarity protein Par3 is mediated by transcription factor Sp1 in breast cancer

2021 ◽  
Vol 561 ◽  
pp. 172-179
Author(s):  
Yannan Zhao ◽  
Dingjin Yao ◽  
Yi Li ◽  
Si Zhang ◽  
Zhonghua Tao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Transcription Factor Sp1
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