Voltage dependence of facilitated arginine flux mediated by the system y+ basic amino acid transporter

Biochemistry ◽  
1993 ◽  
Vol 32 (22) ◽  
pp. 5781-5785 ◽  
Author(s):  
Michael P. Kavanaugh
Keyword(s):  
Amino Acid ◽  
Voltage Dependence ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Acid Transporter

scholarly journals Multiple components of arginine and phenylalanine transport induced in neutral and basic amino acid transporter-cRNA-injected Xenopus oocytes

1996 ◽  
Vol 318 (3) ◽  
pp. 915-922 ◽  
Author(s):  
George J PETER ◽  
Iain G. DAVIDSON ◽  
Aamir AHMED ◽  
Lynn McILROY ◽  
Alexander R. FORRESTER ◽  
...  
Keyword(s):  
Amino Acid ◽  
Xenopus Oocytes ◽  
Competitive Inhibition ◽  
Protein Complexes ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Transport Processes ◽  
Arginine Transport ◽  
Acid Transporter ◽  
Phenylalanine Transport

The induced uptakes of l-[3H]phenylalanine and l-[3H]arginine in oocytes injected with clonal NBAT (neutral and basic amino acid transporter) cRNA show differential inactivation by pre-treatment with N-ethylmaleimide (NEM), revealing at least two distinct transport processes. NEM-resistant arginine transport is inhibited by leucine and phenylalanine but not by alanine or valine; mutual competitive inhibition of NEM-resistant uptake of arginine and phenylalanine indicates that the two amino acids share a single transporter. NEM-senstive arginine transport is inhibited by leucine, phenylalanine, alanine and valine. At least two NEM-sensitive transporters may be expressed because we have been unable to confirm mutual competitive inhibition between arginine and phenylalanine transport. The NEM-resistant transport mechanism appears to involve distinct but overlapping binding sites for cationic and zwitterionic substrates. NBAT is known to form oligomeric protein complexes in cell membranes, and its functional roles when expressed in Xenopus oocytes may include interaction with oocyte proteins, leading to increased native amino acid transport activities; these resemble NBAT-expressed activities in terms of NEM-sensitivity and apparent substrate range (including an unusual inhibition by β-phenylalanine).

scholarly journals Characterization of the rat neutral and basic amino acid transporter utilizing anti-peptide antibodies.

1993 ◽  
Vol 90 (9) ◽  
pp. 4022-4026 ◽  
Author(s):  
R. Mosckovitz ◽  
N. Yan ◽  
E. Heimer ◽  
A. Felix ◽  
S. S. Tate ◽  
...  
Keyword(s):  
Amino Acid ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Acid Transporter ◽  
Peptide Antibodies

Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter

Nature ◽  
1991 ◽  
Vol 352 (6337) ◽  
pp. 729-731 ◽  
Author(s):  
Hao Wang ◽  
Michael P. Kavanaugh ◽  
R. Alan North ◽  
David Kabat
Keyword(s):  
Amino Acid ◽  
Cell Surface ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Cell Surface Receptor ◽  
Surface Receptor ◽  
Acid Transporter

scholarly journals Ultrastructural localization of a neutral and basic amino acid transporter in rat kidney and intestine.

1993 ◽  
Vol 90 (16) ◽  
pp. 7779-7783 ◽  
Author(s):  
V. M. Pickel ◽  
M. J. Nirenberg ◽  
J. Chan ◽  
R. Mosckovitz ◽  
S. Udenfriend ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rat Kidney ◽  
Basic Amino Acid ◽  
Ultrastructural Localization ◽  
Amino Acid Transporter ◽  
Acid Transporter

Immunocytochemical localization of the renal neutral and basic amino acid transporter in rat adrenal gland, brainstem, and spinal cord

1995 ◽  
Vol 356 (4) ◽  
pp. 505-522 ◽  
Author(s):  
Melissa J. Nirenberg ◽  
Suresh S. Tate ◽  
Rachel Mosckovitz ◽  
Sidney Udenfriend ◽  
Virginia M. Pickel
Keyword(s):  
Spinal Cord ◽  
Amino Acid ◽  
Adrenal Gland ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Immunocytochemical Localization ◽  
Acid Transporter

scholarly journals Cloning, functional expression and dietary regulation of the mouse neutral and basic amino acid transporter (NBAT)

1997 ◽  
Vol 328 (2) ◽  
pp. 657-664 ◽  
Author(s):  
Hiroko SEGAWA ◽  
Ken-ichi MIYAMOTO ◽  
Yoshio OGURA ◽  
Hiromi HAGA ◽  
Kyoko MORITA ◽  
...  
Keyword(s):  
Amino Acid ◽  
Xenopus Oocytes ◽  
Physiological Role ◽  
Basic Amino Acid ◽  
Functional Expression ◽  
Amino Acid Transporter ◽  
Amino Acid Transporters ◽  
Transport Activity ◽  
Acid Transporter ◽  
Mouse Ileum

The Na+-independent dibasic and neutral amino acid transporter NBAT is among the least hydrophobic of mammalian amino acid transporters. The transporter contains one to four transmembrane domains and induces amino acid transport activity via a b0,+-like system when expressed in Xenopus oocytes. However, the physiological role of NBAT remains unclear. Complementary DNA clones encoding mouse NBAT have now been isolated. The expression of mouse NBAT in Xenopus oocytes also induced an obligatory amino acid exchange activity similar to that of the b0,+-like system. The amount of NBAT mRNA in mouse kidney increased during postnatal development, consistent with the increase in renal cystine and dibasic transport activity. Dietary aspartate induced a marked increase in cystine transport via the b0,+ system in mouse ileum. A high-aspartate diet also increased the amount of NBAT mRNA in mouse ileum. In the ileum of mice fed on the aspartate diet, the extent of cystine transport was further increased by preloading brush border membrane vesicles with lysine. Hybrid depletion of NBAT mRNA from ileal polyadenylated RNA revealed that the increase in cystine transport activity induced by the high-aspartate diet, as measured in Xenopus oocytes, was attributable to NBAT. These results demonstrate that mouse NBAT has an important role in cystine transport.

scholarly journals Transport Properties of a System y+L Neutral and Basic Amino Acid Transporter

2000 ◽  
Vol 275 (27) ◽  
pp. 20787-20793 ◽  
Author(s):  
Yoshikatsu Kanai ◽  
Yoshiki Fukasawa ◽  
Seok Ho Cha ◽  
Hiroko Segawa ◽  
Arthit Chairoungdua ◽  
...  
Keyword(s):  
Amino Acid ◽  
Transport Properties ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Acid Transporter

The basic amino acid transporter (rBAT) -like immunoreactivity in paraventricular and supraoptic magnocellular neurons of the rat hypothalamus

1996 ◽  
Vol 710 (1-2) ◽  
pp. 299-302 ◽  
Author(s):  
Setsuji Hisano ◽  
Hiromi Haga ◽  
Ken-Ichi Miyamoto ◽  
Eiji Takeda ◽  
Yoshihiro Fukui
Keyword(s):  
Amino Acid ◽  
Basic Amino Acid ◽  
Amino Acid Transporter ◽  
Magnocellular Neurons ◽  
Rat Hypothalamus ◽  
Acid Transporter
Sign in / Sign up

Export Citation Format

Share Document