Thermodynamic characterization of the structural stability of the coiled-coil region of the bZIP transcription factor GCN4

Biochemistry ◽  
1993 ◽  
Vol 32 (21) ◽  
pp. 5491-5496 ◽  
Author(s):  
Kelly S. Thompson ◽  
Charles R. Vinson ◽  
Ernesto Freire
Keyword(s):  
Transcription Factor ◽  
Structural Stability ◽  
Coiled Coil ◽  
Bzip Transcription Factor ◽  
Thermodynamic Characterization ◽  
Coiled Coil Region

scholarly journals 2066 Functional characterization of mutant BRCA1

2018 ◽  
Vol 2 (S1) ◽  
pp. 13-13
Author(s):  
John Barrows ◽  
David Long
Keyword(s):  
Functional Characterization ◽  
Coiled Coil ◽  
Wild Type ◽  
Coiled Coil Region ◽  
Egg Extracts ◽  
Study Population ◽  
Xenopus Egg ◽  
Xenopus Egg Extracts

OBJECTIVES/SPECIFIC AIMS: The objective of this work is to determine the mechanistic consequences of BRCA1 mutants in inter-strand crosslink (ICL) repair. METHODS/STUDY POPULATION: Our lab uses Xenopus egg extracts to study ICL repair. These extracts can be depleted of endogenous BRCA1 by immunoprecipitation. The goal of this work is to rescue endogenous depletion with in vitro translated, wild type BRCA1. Once achieved, we can supplement the depleted extract with BRCA1 mutants to access their function in ICL repair. RESULTS/ANTICIPATED RESULTS: We hypothesize that the BRCT and RING domain mutations will abrogate ICL repair, while mutations in the coiled coil region will not affect repair. DISCUSSION/SIGNIFICANCE OF IMPACT: These findings will have an immense impact on the understanding of BRCA1 domains. Importantly these results will spur personalized therapy of BRCA1 mutants by showing which domains are sensitive to cross-linking agents.

Cloning and characterization of the mouse polyamine-modulatedfactor-1 (mPMF-1) gene: an alternatively spliced homologue of the human transcription factor

2001 ◽  
Vol 359 (2) ◽  
pp. 387-392 ◽  
Author(s):  
Yanlin WANG ◽  
Wendy DEVEREUX ◽  
Patrick M. WOSTER ◽  
Robert A. CASERO
Keyword(s):  
Amino Acids ◽  
Transcription Factor ◽  
Transcriptional Activation ◽  
Splice Variants ◽  
Coiled Coil ◽  
Human Protein ◽  
Related Factor ◽  
Polyamine Analogues ◽  
Coiled Coil Region ◽  
Alternatively Spliced

The natural polyamines and their analogues have been implicated in transcriptional regulation of specific genes. Human polyamine-modulated factor-1 (hPMF-1) was the first polyamine-responsive transcription factor identified. Here the mouse homologue of the hPMF-1 gene is described. Interestingly, the mouse gene (mPMF-1) codes for two alternatively spliced mRNAs. Both of the mouse splice variants, mPMF-1S and mPMF-1L, possess C-terminal coiled-coil domains nearly identical to that found in hPMF-1 and are highly homologous with the human protein. The C-terminal coiled-coil structure is necessary for transcriptional activation. However, the shorter protein, mPMF-1S, does not contain an N-terminal coiled-coil region as do both hPMF-1 and the longer mPMF-1L. mPMF-1L mRNA codes for a protein of 202 amino acids, 37 amino acids longer than the human protein. By contrast, mPMF-1S codes for only 133 amino acids, as a result of two exons being omitted compared with mPMF-1L. Both mouse transcription factors can interact with Nrf-2 (nuclear factor-E2-related factor 2), the normal partner of hPMF-1, substantiating the importance of the C-terminal coiled-coil region responsible for this interaction. Finally, the expression of mPMF-1 is induced when mouse M1 myeloid leukaemia cells are exposed to polyamine analogues, suggesting control similar to that observed for the hPMF-1.

Molecular characterization of a stress-response bZIP transcription factor in banana

2012 ◽  
Vol 113 (2) ◽  
pp. 173-187 ◽  
Author(s):  
Sen He ◽  
Wei Shan ◽  
Jian-fei Kuang ◽  
Hui Xie ◽  
Yun-yi Xiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Response ◽  
Molecular Characterization ◽  
Bzip Transcription Factor
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