Structure, 5'-flanking sequence, and chromosome location of the human N-formyl peptide receptor gene. A single-copy gene comprised of two exons on chromosome 19q.13.3 that yields two distinct transcripts by alternative polyadenylation

Biochemistry ◽  
1993 ◽  
Vol 32 (16) ◽  
pp. 4168-4174 ◽  
Author(s):  
David L. Haviland ◽  
Angela C. Borel ◽  
Daniel T. Fleischer ◽  
Joie C. Haviland ◽  
Rick A. Wetsel
Keyword(s):  
Receptor Gene ◽  
Alternative Polyadenylation ◽  
Single Copy ◽  
Formyl Peptide Receptor ◽  
Single Copy Gene ◽  
Flanking Sequence ◽  
Peptide Receptor ◽  
Copy Gene ◽  
Formyl Peptide ◽  
Chromosome 19Q

The utility and application of real-time PCR and FISH in the detection of single-copy gene targets inEscherichia coliO157:H7 andSalmonellaTyphimurium

2010 ◽  
Vol 56 (3) ◽  
pp. 254-262 ◽  
Author(s):  
Merriam Haffar ◽  
Kimberley A. Gilbride
Keyword(s):  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Receptor Gene ◽  
Detection Limits ◽  
Single Copy ◽  
Target Cells ◽  
Single Copy Gene ◽  
Gene Copies ◽  
Copy Gene

The ultimate specificity in molecular-based assays for pathogen detection relies on the design of the primers and probes. Their ability to hybridize to DNA sequences found only in pathogens can be realized by designing primers and probes that are complementary to pathogen-specific virulence genes. This study evaluates the detection and enumeration strengths of real-time PCR (qPCR) and fluorescent in situ hybridization (FISH) for selected waterborne pathogens and their ultimate applicability within a monitoring framework. Detection limits calculated in the qPCR assay were 150 tir (intimin protein receptor) gene copies for Escherichia coli O157:H7 and 2 × 103invA (inner membrane invasive protein) gene copies for Salmonella enterica serovar Typhimurium. Detection limits were, however, at least 100-fold less sensitive in wastewater extracts, partly because of the inhibitory effect of the wastewater itself. Fluorescent signals from hybridized whole target cells were below the detection limit of the FISH assay. While this research demonstrates the potential detection strength of qPCR, it highlights the need for strong dependable primer and probe sets among PCR and FISH methodologies as well as the need for further signal amplification with DNA-targeted FISH for single-copy gene targets within environmental samples.

scholarly journals Characterization of Fpr-rs8, an Atypical Member of the Mouse Formyl Peptide Receptor Gene Family

2011 ◽  
Vol 3 (5) ◽  
pp. 519-529 ◽  
Author(s):  
H. Lee Tiffany ◽  
Ji-Liang Gao ◽  
Ester Roffe ◽  
Joan M.G. Sechler ◽  
Philip M. Murphy
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Formyl Peptide Receptor ◽  
Peptide Receptor ◽  
Formyl Peptide ◽  
Atypical Member ◽  
Receptor Gene Family

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene
Sign in / Sign up

Export Citation Format

Share Document