Regeneration of bovine pancreatic ribonuclease A. 1. Steady-state distribution

Biochemistry ◽  
1993 ◽  
Vol 32 (10) ◽  
pp. 2671-2679 ◽  
Author(s):  
David M. Rothwarf ◽  
Harold A. Scheraga
Keyword(s):  
Steady State ◽  
Ribonuclease A ◽  
Steady State Distribution ◽  
State Distribution ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A

Kinetic analysis of mechanism of intestinal Na+-dependent sugar transport

1985 ◽  
Vol 248 (5) ◽  
pp. C498-C509 ◽  
Author(s):  
D. Restrepo ◽  
G. A. Kimmich
Keyword(s):  
Experimental Data ◽  
Steady State ◽  
Sugar Transport ◽  
Enzyme Kinetic ◽  
Steady State Distribution ◽  
State Distribution ◽  
Least Squares Fit ◽  
Coupling Stoichiometry ◽  
Rate Limiting ◽  
Kinetics Of

Zero-trans kinetics of Na+-sugar cotransport were investigated. Sugar influx was measured at various sodium and sugar concentrations in K+-loaded cells treated with rotenone and valinomycin. Sugar influx follows Michaelis-Menten kinetics as a function of sugar concentration but not as a function of Na+ concentration. Nine models with 1:1 or 2:1 sodium:sugar stoichiometry were considered. The flux equations for these models were solved assuming steady-state distribution of carrier forms and that translocation across the membrane is rate limiting. Classical enzyme kinetic methods and a least-squares fit of flux equations to the experimental data were used to assess the fit of the different models. Four models can be discarded on this basis. Of the remaining models, we discard two on the basis of the trans sodium dependence and the coupling stoichiometry [G. A. Kimmich and J. Randles, Am. J. Physiol. 247 (Cell Physiol. 16): C74-C82, 1984]. The remaining models are terter ordered mechanisms with sodium debinding first at the trans side. If transfer across the membrane is rate limiting, the binding order can be determined to be sodium:sugar:sodium.

scholarly journals On the Preparation of Bovine Pancreatic Ribonuclease A

1963 ◽  
Vol 238 (2) ◽  
pp. 618-621 ◽  
Author(s):  
Arthur M. Crestfield ◽  
William H. Stein ◽  
Stanford Moore
Keyword(s):  
Ribonuclease A ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A

scholarly journals The Amino Acid Sequence of Ovine Pancreatic Ribonuclease A

1973 ◽  
Vol 248 (22) ◽  
pp. 7833-7837
Author(s):  
Ryoko Kobayashi ◽  
C.H.W. Hirs ◽  
With the technical assistance of Marie Hagenhuber
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Ribonuclease A ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A

New monoclinic form of bovine pancreatic ribonuclease A from a salt solution and comparison of intermolecular interactions in ribonucleases A

2011 ◽  
Vol 319 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Hideaki Takusagawa ◽  
Shigefumi Yamamura ◽  
Shigeru Endo ◽  
Yoko Sugawara ◽  
Toshiyuki Inagaki ◽  
...  
Keyword(s):  
Intermolecular Interactions ◽  
Salt Solution ◽  
Ribonuclease A ◽  
Pancreatic Ribonuclease ◽  
Monoclinic Form ◽  
Pancreatic Ribonuclease A

Local and Long-Range Interactions in the Thermal Unfolding Transition of Bovine Pancreatic Ribonuclease A†

Biochemistry ◽  
2001 ◽  
Vol 40 (1) ◽  
pp. 93-104 ◽  
Author(s):  
Amiel Navon ◽  
Varda Ittah ◽  
John H. Laity ◽  
Harold A. Scheraga ◽  
Elisha Haas ◽  
...  
Keyword(s):  
Long Range ◽  
Ribonuclease A ◽  
Thermal Unfolding ◽  
Pancreatic Ribonuclease ◽  
Long Range Interactions ◽  
Pancreatic Ribonuclease A
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