Acid Denaturation of Recombinant Porcine Growth Hormone:  Formation and Self-Association of Folding Intermediates†

Biochemistry ◽  
2000 ◽  
Vol 39 (40) ◽  
pp. 12345-12354 ◽  
Author(s):  
Emma J. Parkinson ◽  
Michael B. Morris ◽  
Stan Bastiras
Keyword(s):  
Growth Hormone ◽  
Folding Intermediates ◽  
Acid Denaturation ◽  
Self Association ◽  
Hormone Formation

Weak self-association of human growth hormone investigated by nitrogen-15 NMR relaxation

2008 ◽  
Vol 73 (1) ◽  
pp. 161-172 ◽  
Author(s):  
Malene Ringkjøbing Jensen ◽  
Søren M. Kristensen ◽  
Camille Keeler ◽  
Hans E. M. Christensen ◽  
Michael E. Hodsdon ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Nmr Relaxation ◽  
Human Growth ◽  
Self Association

scholarly journals Prolactin and Growth Hormone Aggregates in Secretory Granules: The Need to Understand the Structure of the Aggregate

2012 ◽  
Vol 33 (2) ◽  
pp. 254-270 ◽  
Author(s):  
Priscilla S. Dannies
Keyword(s):  
Growth Hormone ◽  
Secretory Granules ◽  
Macromolecular Crowding ◽  
Acidic Ph ◽  
Wild Type ◽  
Amyloid Fibers ◽  
Dense Cores ◽  
Reversible Aggregation ◽  
Human Prolactin ◽  
Self Association

Prolactin and GH form reversible aggregates in the trans-Golgi lumen that become the dense cores of secretory granules. Aggregation is an economical means of sorting, because self-association removes the hormones from other possible pathways. Secretory granules containing different aggregates show different behavior, such as the reduction in stimulated release of granules containing R183H-GH compared with release of those containing wild-type hormone. Aggregates may facilitate localization of membrane proteins necessary for transport and exocytosis of secretory granules, and therefore understanding their properties is important. Three types of self-association have been characterized: dimers of human GH that form with Zn2+, low-affinity self-association of human prolactin caused by acidic pH and Zn2+ with macromolecular crowding, and amyloid fibers of prolactin. The best candidate for the form in most granules may be low-affinity self-association because it occurs rapidly at Zn2+ concentrations that are likely to be in granules and reverses rapidly in neutral pH. Amyloid may form in older granules. Determining differences between aggregates of wild type and those of R183H-GH should help to understand why granules containing the mutant behave differently from those containing wild-type hormone. If reversible aggregation of other hormones, including those that are proteolytically processed, is the crucial act in forming granules, rather than use of a sorting signal, then prohormones should form reversible aggregates in solution in conditions that resemble those of the trans-Golgi lumen, including macromolecular crowding.

Reversible self-association of bovine growth hormone during equilibrium unfolding

Biochemistry ◽  
1986 ◽  
Vol 25 (21) ◽  
pp. 6533-6538 ◽  
Author(s):  
H. A. Havel ◽  
E. W. Kauffman ◽  
S. M. Plaisted ◽  
D. N. Brems
Keyword(s):  
Growth Hormone ◽  
Bovine Growth Hormone ◽  
Equilibrium Unfolding ◽  
Self Association

Acid stabilization of human growth hormone equilibrium folding intermediates

1995 ◽  
Vol 1247 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Michael R. DeFelippis ◽  
Maureen A. Kilcomons ◽  
Mary P. Lents ◽  
Karen M. Youngman ◽  
Henry A. Havel
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Folding Intermediates

Association of pituitary adenoma with neuronal christoma: A TEM study

1994 ◽  
Vol 52 ◽  
pp. 232-233
Author(s):  
Eva Horvath ◽  
Kalman Kovacs ◽  
B. W. Scheithauer ◽  
R. V. Lloyd ◽  
H. S. Smyth
Keyword(s):  
Growth Hormone ◽  
Pituitary Adenoma ◽  
Pituitary Tumor ◽  
Nervous Tissue ◽  
Alternative Explanation ◽  
Growth Hormone Releasing Hormone ◽  
Cell Hyperplasia ◽  
Releasing Hormone ◽  
Secretory Neurons

The association of a pituitary adenoma with nervous tissue consisting of neuron-like cells and neuropil is a rare abnormality. In the majority of cases, the pituitary tumor is a chromophobic adenoma, accompanied by acromegaly. Histology reveals widely variable proportions of endocrine and nervous tissue in alternating or intermingled patterns. The lesion is perceived as a composite one consisting of two histogenetically distinct parts. It has been suggested that the neuronal component, morphologically similar to secretory neurons of the hypothalamus, may initiate adenoma formation by releasing stimulatory substances. Immunoreactivity for growth hormone releasing hormone (GRH) in the neuronal component of some cases supported this view, whereas other findings such as consistent lack of growth hormone (GH) cell hyperplasia in the lesions called for alternative explanation.Fifteen tumors consisting of a pituitary adenoma and a neuronal component have been collected over a 20 yr. period. Acromegaly was present in 11 patients, was equivocal in one, and absent in 3.

Target Growth Hormone Doses to IGF-1 Levels

2005 ◽  
Vol 39 (10) ◽  
pp. 10
Author(s):  
BETSY BATES
Keyword(s):  
Growth Hormone
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