Catalytic Properties of Hairpin Ribozymes Derived from Chicory Yellow Mottle Virus and Arabis Mosaic Virus Satellite RNAs

Biochemistry ◽  
1995 ◽  
Vol 34 (48) ◽  
pp. 15785-15791 ◽  
Author(s):  
Mary Beth DeYoung ◽  
Andrew M. Siwkowski ◽  
Ying Lian ◽  
Arnold Hampel
Keyword(s):  
Mosaic Virus ◽  
Catalytic Properties ◽  
Mottle Virus ◽  
Arabis Mosaic Virus ◽  
Yellow Mottle ◽  
Satellite Rnas ◽  
Hairpin Ribozymes

Sequence elements outside the catalytic core of natural hairpin ribozymes modulate the reactions differentially

2011 ◽  
Vol 392 (7) ◽  
Author(s):  
Preeti Bajaj ◽  
Gerhard Steger ◽  
Christian Hammann
Keyword(s):  
Mutational Analysis ◽  
Hammerhead Ribozyme ◽  
The Other ◽  
Hairpin Ribozyme ◽  
Catalytic Core ◽  
Arabis Mosaic Virus ◽  
Sequence Elements ◽  
Satellite Rnas ◽  
Hairpin Ribozymes

AbstractHairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show here that sCYMoV features a five-way helical junction instead. Mutational analysis indicates that the fifth stem does not influence kinetic parameters of the sCYMoV hairpin ribozymein vitroreactions, and therefore seems an appendix to that junction in the other ribozymes. We report further that all three ribozymes feature a three-way helical junction outside the catalytic core in stem A, with Watson-Crick complementarity to loop nucleotides in stem B. Kinetic analyses of cleavage and ligation reactions of several variants of the sTRsV and sCYMoV hairpin ribozymesin vitroshow that the presence of this junction interferes with their reactions, particularly the ligation. We provide evidence that this is not due to a presumed interaction of the afore-mentioned elements in stems A and B. The evolutionary survival of thiscis-inhibiting element seems rather to be caused by the coincidence of its position with that of the hammerhead ribozyme in the other RNA polarity.

scholarly journals DETEKSI VIRUS PADA BIBIT LADA (Piper nigrum) DENGAN TEKNIK ELISA

2017 ◽  
Vol 27 (2) ◽  
pp. 157 ◽  
Author(s):  
Maya Mariana ◽  
NFN Miftakhurohmah
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mottle Virus ◽  
Piper Nigrum ◽  
Yellow Mottle

<p class="IsiabstrakIndonesia">Salah satu penyakit penting pada tanaman lada yaitu penyakit kerdil yang disebabkan oleh <em>Piper yellow mottle virus </em>(PYMoV) dan <em>Cucumber mosaic virus </em>(CMV). Tindakan pencegahan penyebaran penyakit ini dapat dilakukan dengan penggunaan bibit sehat. Deteksi dini PYMoV dan CMV pada bibit lada merupakan salah satu cara untuk mendapatkan bibit sehat, yang dapat dilakukan secara serologi menggunakan teknik ELISA. Tujuan penelitian adalah mendeteksi keberadaan virus pada bibit lada siap tanam pada pembibitan di Kabupaten Sukabumi dan Purbalingga. Sampel daun yang diuji diambil dari bibit lada berumur kurang lebih 5 bulan. Varietas yang diamati adalah Natar 1, Petaling, dan Lampung Daun Kecil. Hasil pengujian menunjukkan bahwa bibit lada terinfeksi virus di kedua lokasi pembibitan.</p>

scholarly journals DETEKSI CMV DAN PYMoV PADA BENIH LADA (Piper nigrum) DENGAN TEKNIK ELISA

2017 ◽  
Vol 27 (2) ◽  
pp. 155
Author(s):  
Maya Mariana ◽  
NFN Miftakhurohmah
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mottle Virus ◽  
Piper Nigrum ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
Double Antibody ◽  
Yellow Mottle ◽  
Das Elisa

<p class="IsiabstrakIndonesia">Salah satu kendala penting pada budidaya tanaman lada yaitu penyakit kerdil yang disebabkan oleh <em>Piper yellow mottle virus </em>(PYMoV) dan <em>Cucumber mosaic virus </em>(CMV). Tindakan pencegahan penyebaran penyakit dapat dilakukan salah satunya dengan penggunaan benih sehat bebas dari infeksi PYMoV dan CMV. Tujuan penelitian adalah mendeteksi keberadaan PYMoV dan CMV pada benih lada siap tanam secara serologi menggunakan teknik ELISA. Benih lada yang dideteksi berumur 5 bulan, berasal dari penangkar benih di Sukabumi (Jawa Barat) dan Purbalingga (Jawa Tengah). Varietas lada yang diambil di Sukabumi adalah Natar 1, Petaling, dan Lampung Daun Kecil., masing-masing sebanyak 10 sampel, sedangkan di Purbalingga hanya ada Natar 1, diambil 30 sampel. Di setiap lokasi pembibitan juga dilakukan pengamatan terhadap gejala infeksi virus yang ditemukan. Deteksi virus dilakukan secara <em>Double Antibody Sandwich</em> (DAS)-ELISA menggunakan antiserum <em>Banana streak virus</em> (BSV) untuk PYMoV dan antiserum CMV untuk deteksi CMV. Hasil ELISA dibaca nilai absorbannya dengan elisa reader pada panjang gelombang 405 nm. Sampel dinilai positif, jika nilai absorbansinya 1,5 kali lebih besar daripada kontrol negatif. Gejala infeksi virus pada benih bervariasi, yaitu klorotik, belang, dan belang disertai perubahan bentuk daun. Hasil pengujian ELISA menunjukkan bahwa 66% benih lada dari Sukabumi dan 46% dari Purbalingga terinfeksi virus CMV. Namun, tidak ada satu pun yang menunjukkan reaksi positif terhadap antiserum PYMoV. Hal ini diduga karena konsentrasi PYMoV terlalu rendah sehingga tidak terdeteksi secara ELISA. Untuk mencegah penyebaran virus, deteksi virus pada benih lada penting dilakukan sebelum digunakan sebagai bahan tanaman. </p>

scholarly journals Occurrence of cowpea viruses in the forest and savannah agro-ecological zones of Ghana

1970 ◽  
Vol 28 (3) ◽  
pp. 441-448
Author(s):  
F.K. Adams ◽  
L. Kumar ◽  
C. Kwoseh ◽  
R. Akromah
Keyword(s):  
Mosaic Virus ◽  
Sub Saharan Africa ◽  
Mottle Virus ◽  
Grain Legume ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ecological Zones ◽  
Coated Plate ◽  
Yellow Mottle ◽  
Sub Saharan ◽  
Blackeye Cowpea Mosaic Virus

Cowpea (Vigna unguiculata (L.) Walp) is a major grain legume in Sub Saharan Africa whose production is heavily affected by viral diseases. A disease survey and antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) were used to determine the incidence, severity and occurrence of viruses infecting cowpea in some major growing areas in the Ashanti and Brong Ahafo regions of Ghana. The survey revealed the highest virus incidence (81.6%) and severity (3.01) in Ejura, and the least incidence (46.7%) and severity (2.38) in Nkoranza. The ACP-ELISA results indicated that Blackeye cowpea mosaic virus (BICMV) was the most prevalent virus, with occurrence ranging from 32% in Mampong to 60% in Atebubu. Cucumber mosaic virus (CMV) was the least detected, occurring only in samples from Nkoranza (4%). Cowpea mild mottle virus (CPMMV), Cowpea mottle virus (CPMoV), Southern bean mosaic virus (SBMV), Cowpea aphid borne mosaic virus (CABMV) and Cowpea yellow mottle virus (CYMV) were also detected in the samples. The prevalence of these viruses suggest the need for continuous monitoring of cowpea fields in the country to ensure effective disease management.

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