Reconstitution of the Endogenous Plastoquinone Pool in Photosystem II (PS II) Membrane Fragments, Inside-Out-Vesicles, and PS II Core Complexes from Spinach

Biochemistry ◽  
1995 ◽  
Vol 34 (48) ◽  
pp. 15721-15731 ◽  
Author(s):  
J. Kurreck ◽  
A. G. Seeliger ◽  
F. Reifarth ◽  
M. Karge ◽  
G. Renger
Keyword(s):  
Photosystem Ii ◽  
Ps Ii ◽  
Plastoquinone Pool ◽  
Inside Out ◽  
Ps Ii Core Complexes ◽  
Ps Ii Core

Effects of hydrogen/deuterium exchange on photosynthetic water cleavage in PS II core complexes from spinach

FEBS Letters ◽  
1996 ◽  
Vol 378 (2) ◽  
pp. 140-144 ◽  
Author(s):  
M. Karge ◽  
K.-D. Irrgang ◽  
S. Sellin ◽  
R. Feinäugle ◽  
B. Liu ◽  
...  
Keyword(s):  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Ps Ii ◽  
Hydrogen Deuterium ◽  
Water Cleavage ◽  
Ps Ii Core Complexes ◽  
Ps Ii Core

Significance of Photosystem II Core Phosphorylation Heterogeneity for the Herbicide-Binding Domain

1993 ◽  
Vol 48 (3-4) ◽  
pp. 241-245 ◽  
Author(s):  
Maria Teresa Giardi
Keyword(s):  
Photosystem Ii ◽  
Spinacia Oleracea ◽  
Binding Activity ◽  
Alternative Methods ◽  
Relative Distribution ◽  
Ps Ii ◽  
Spinacia Oleracea L ◽  
Herbicide Binding ◽  
Ps Ii Core

Abstract In recent papers the heterogeneous nature of photosystem (PS) II core phosphorylation has been revealed (Giardi et al., BBRC 176, 1298 -1305 (1991); Plant Physiol. 100, 1948 -1954 (1992)). In this paper the action of endogenous and exogenous phosphatases both on the distribution of phosphorylated PS II core populations and on herbicide-binding activity in photosystem II preparations from Spinacia oleracea L. has been investigated. The results indicate that these phosphatases modify the photosystem II core phosphorylation heterogeneity at a different level. Dark incubation causes a partial dephosphorylation of D1 and D2 proteins by endogenous phosphatase(s) and changes the relative distribution of phosphorylated photosystem II core populations, while the action of the alkaline phosphatase leads to extensive dephosphorylation and to the detachment of PsbH protein from the photosystem II core. Dephosphorylation by the two alternative methods results in a differential modification of herbicide-binding activity. It is suggested that photosystem II heterogeneity with respect to the herbicide action, observed in vivo, could be a consequence of PS II core phosphorylation heterogeneity.

Sign in / Sign up

Export Citation Format

Share Document