Expression and Purification of the Light Chain of Botulinum Neurotoxin A: A Single Mutation Abolishes Its Cleavage of SNAP-25 and Neurotoxicity after Reconstitution with the Heavy Chain

Biochemistry ◽  
1995 ◽  
Vol 34 (46) ◽  
pp. 15175-15181 ◽  
Author(s):  
Liqing Zhou ◽  
Anton de Paiva ◽  
Dandan Liu ◽  
Roger Aoki ◽  
J. Oliver Dolly
Keyword(s):  
Heavy Chain ◽  
Light Chain ◽  
Botulinum Neurotoxin ◽  
Single Mutation ◽  
Expression And Purification ◽  
Botulinum Neurotoxin A

scholarly journals Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library

mAbs ◽  
2014 ◽  
Vol 6 (2) ◽  
pp. 446-459 ◽  
Author(s):  
Sebastian Miethe ◽  
Christine Rasetti-Escargueil ◽  
Yvonne Liu ◽  
Siham Chahboun ◽  
Thibaut Pelat ◽  
...  
Keyword(s):  
Light Chain ◽  
Botulinum Neurotoxin ◽  
Botulinum Neurotoxin A ◽  
Immune Library

scholarly journals Biofunctionalized Gold Nanoparticles for Colorimetric Sensing of Botulinum Neurotoxin A Light Chain

2014 ◽  
Vol 86 (5) ◽  
pp. 2345-2352 ◽  
Author(s):  
Xiaohu Liu ◽  
Yi Wang ◽  
Peng Chen ◽  
Yusong Wang ◽  
Jinling Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Light Chain ◽  
Botulinum Neurotoxin ◽  
Colorimetric Sensing ◽  
Botulinum Neurotoxin A

scholarly journals Cloning, expression and purification of botulinum neurotoxin type A heavy chain - crystallographic evidence for a putative tetrameric pore

2012 ◽  
Vol 2 (2) ◽  
pp. 135
Author(s):  
Damodharan Lakshminarasimhan ◽  
Desigan Kumaran ◽  
Rakhi Agarwal ◽  
Bal Ram Singh ◽  
Subramanyam Swaminathan
Keyword(s):  
Heavy Chain ◽  
Botulinum Neurotoxin ◽  
Type A ◽  
Expression And Purification ◽  
Botulinum Neurotoxin Type A

scholarly journals Time-resolved botulinum neurotoxin A activity monitored using peptide-functionalized Au nanoparticle energy transfer sensors

2014 ◽  
Vol 5 (7) ◽  
pp. 2651-2656 ◽  
Author(s):  
Yi Wang ◽  
Xiaohu Liu ◽  
Jinling Zhang ◽  
Daniel Aili ◽  
Bo Liedberg
Keyword(s):  
Energy Transfer ◽  
Light Chain ◽  
Botulinum Neurotoxin ◽  
Au Nanoparticles ◽  
Au Nanoparticle ◽  
Botulinum Neurotoxin A ◽  
Time Resolved

A peptide mimicking SNAP-25 was immobilized on Au nanoparticles for the monitoring of botulinum neurotoxin light chain A activity.

Toward the discovery of dual inhibitors for botulinum neurotoxin A: concomitant targeting of endocytosis and light chain protease activity

2015 ◽  
Vol 51 (28) ◽  
pp. 6226-6229 ◽  
Author(s):  
Hajime Seki ◽  
Song Xue ◽  
Mark S. Hixon ◽  
Sabine Pellett ◽  
Marek Remes̆ ◽  
...  
Keyword(s):  
Light Chain ◽  
Protease Activity ◽  
Botulinum Neurotoxin ◽  
Botulinum Neurotoxin A ◽  
Dual Inhibitors

A polypharmacological approach as a treatment for botulinum neurotoxin A intoxication is presented.

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