Mechanistic Studies of the Methyltransferase from Clostridium thermoaceticum: Origin of the pH Dependence of the Methyl Group Transfer from Methyl Tetrahydrofolate to the Corrinoid/Iron-Sulfur Protein

Shaying Zhao; David Lee Roberts; Stephen W. Ragsdale

doi:10.1021/bi00046a013

Mechanistic Studies of the Methyltransferase from Clostridium thermoaceticum: Origin of the pH Dependence of the Methyl Group Transfer from Methyl Tetrahydrofolate to the Corrinoid/Iron-Sulfur Protein

Biochemistry ◽

10.1021/bi00046a013 ◽

1995 ◽

Vol 34 (46) ◽

pp. 15075-15083 ◽

Cited By ~ 32

Author(s):

Shaying Zhao ◽

David Lee Roberts ◽

Stephen W. Ragsdale

Keyword(s):

Ph Dependence ◽

Mechanistic Studies ◽

Clostridium Thermoaceticum ◽

Methyl Group ◽

Group Transfer ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein ◽

Methyl Tetrahydrofolate ◽

Methyl Group Transfer

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Stopped-Flow Kinetics of Methyl Group Transfer between the Corrinoid-Iron-Sulfur Protein and Acetyl-Coenzyme A Synthase fromClostridiumthermoaceticum

Journal of the American Chemical Society ◽

10.1021/ja016676r ◽

2002 ◽

Vol 124 (22) ◽

pp. 6277-6284 ◽

Cited By ~ 36

Author(s):

Xiang Shi Tan ◽

Christopher Sewell ◽

Paul A. Lindahl

Keyword(s):

Stopped Flow ◽

Acetyl Coenzyme A ◽

Methyl Group ◽

Group Transfer ◽

Iron Sulfur ◽

Sulfur Protein ◽

Acetyl Coenzyme A Synthase ◽

Iron Sulfur Protein ◽

Kinetics Of ◽

Methyl Group Transfer

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Sequence and expression of the gene encoding the corrinoid/iron-sulfur protein from Clostridium thermoaceticum and reconstitution of the recombinant protein to full activity.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)53364-0 ◽

1993 ◽

Vol 268 (8) ◽

pp. 5605-5614

Author(s):

W.P. Lu ◽

I. Schiau ◽

J.R. Cunningham ◽

S.W. Ragsdale

Keyword(s):

Recombinant Protein ◽

Clostridium Thermoaceticum ◽

Gene Encoding ◽

Full Activity ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)32932-6 ◽

1983 ◽

Vol 258 (4) ◽

pp. 2364-2369 ◽

Cited By ~ 16

Author(s):

S W Ragsdale ◽

J E Clark ◽

L G Ljungdahl ◽

L L Lundie ◽

H L Drake

Keyword(s):

Carbon Monoxide ◽

Carbon Monoxide Dehydrogenase ◽

Clostridium Thermoaceticum ◽

Nickel Iron ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein

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The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum.

Journal of Bacteriology ◽

10.1128/jb.176.19.6127-6130.1994 ◽

1994 ◽

Vol 176 (19) ◽

pp. 6127-6130 ◽

Cited By ~ 33

Author(s):

D L Roberts ◽

S Zhao ◽

T Doukov ◽

S W Ragsdale

Keyword(s):

Heterologous Expression ◽

Coenzyme A ◽

Clostridium Thermoaceticum ◽

Acetyl Coenzyme A ◽

Acetyl Coenzyme ◽

Iron Sulfur ◽

Protein Methyltransferase ◽

Sulfur Protein ◽

Iron Sulfur Protein

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X-ray absorption spectroscopy of the corrinoid/iron-sulfur protein involved in acetyl coenzyme A synthesis by Clostridium thermoaceticum

Journal of the American Chemical Society ◽

10.1021/ja00059a007 ◽

1993 ◽

Vol 115 (6) ◽

pp. 2146-2150 ◽

Cited By ~ 21

Author(s):

Michael D. Wirt ◽

Manoj Kumar ◽

Stephen W. Ragsdale ◽

Mark R. Chance

Keyword(s):

Absorption Spectroscopy ◽

Coenzyme A ◽

Clostridium Thermoaceticum ◽

Acetyl Coenzyme A ◽

X Ray ◽

Acetyl Coenzyme ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein ◽

X Ray Absorption

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Controlled potential enzymology of methyl transfer reactions involved in acetyl-CoA synthesis by CO dehydrogenase and the corrinoid/iron-sulfur protein from Clostridium thermoaceticum.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39743-1 ◽

1990 ◽

Vol 265 (6) ◽

pp. 3124-3133 ◽

Cited By ~ 3

Author(s):

W P Lu ◽

S R Harder ◽

S W Ragsdale

Keyword(s):

Transfer Reactions ◽

Clostridium Thermoaceticum ◽

Co Dehydrogenase ◽

Acetyl Coa ◽

Iron Sulfur ◽

Methyl Transfer ◽

Acetyl Coa Synthesis ◽

Controlled Potential ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Mössbauer, EPR, and optical studies of the corrinoid/iron-sulfur protein involved in the synthesis of acetyl coenzyme A by Clostridium thermoaceticum.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47936-7 ◽

1987 ◽

Vol 262 (29) ◽

pp. 14289-14297 ◽

Cited By ~ 8

Author(s):

S W Ragsdale ◽

P A Lindahl ◽

E Münck

Keyword(s):

Coenzyme A ◽

Clostridium Thermoaceticum ◽

Optical Studies ◽

Acetyl Coenzyme A ◽

Acetyl Coenzyme ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Binding of Carbon Disulfide to the Site of Acetyl-CoA Synthesis by the Nickel-Iron-Sulfur Protein, Carbon Monoxide Dehydrogenase, from Clostridium thermoaceticum

Biochemistry ◽

10.1021/bi00198a048 ◽

1994 ◽

Vol 33 (32) ◽

pp. 9769-9777 ◽

Cited By ~ 29

Author(s):

Manoj Kumar ◽

Wei-Ping Lu ◽

Stephen W. Ragsdale

Keyword(s):

Carbon Monoxide ◽

Carbon Disulfide ◽

Carbon Monoxide Dehydrogenase ◽

Clostridium Thermoaceticum ◽

Acetyl Coa ◽

Nickel Iron ◽

Iron Sulfur ◽

Acetyl Coa Synthesis ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Spectroelectrochemical studies of the corrinoid/iron-sulfur protein involved in acetyl coenzyme A synthesis by Clostridium thermoaceticum

Biochemistry ◽

10.1021/bi00449a019 ◽

1989 ◽

Vol 28 (23) ◽

pp. 9080-9087 ◽

Cited By ~ 60

Author(s):

Scott R. Harder ◽

Wei Ping Lu ◽

Benjamin A. Feinberg ◽

Stephen W. Ragsdale

Keyword(s):

Coenzyme A ◽

Clostridium Thermoaceticum ◽

Acetyl Coenzyme A ◽

Acetyl Coenzyme ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Structural and Electronic Factors in Heterolytic Cleavage: Formation of the Co(I) Intermediate in the Corrinoid/Iron-Sulfur Protein from Clostridium thermoaceticum

Biochemistry ◽

10.1021/bi00015a042 ◽

1995 ◽

Vol 34 (15) ◽

pp. 5269-5273 ◽

Cited By ~ 11

Author(s):

Michael D. Wirt ◽

Manoj Kumar ◽

Jing-jing Wu ◽

Eva M. Scheuring ◽

Stephen W. Ragsdale ◽

...

Keyword(s):

Clostridium Thermoaceticum ◽

Heterolytic Cleavage ◽

Iron Sulfur ◽

Sulfur Protein ◽

Iron Sulfur Protein

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