Structure-function assessment of the role of the helical stack domain in the properties of human recombinant protein C and activated protein C

Biochemistry ◽  
1995 ◽  
Vol 34 (25) ◽  
pp. 8082-8090 ◽  
Author(s):  
William T. Christiansen ◽  
Jie-Ping Geng ◽  
Francis J. Castellino
Keyword(s):  
Recombinant Protein ◽  
Structure Function ◽  
Protein C ◽  
Activated Protein C ◽  
Human Recombinant Protein ◽  
Function Assessment

Protective role of activated protein C in lung and airway remodeling

2004 ◽  
Vol 32 (Supplement) ◽  
pp. S262-S265 ◽  
Author(s):  
Koji Suzuki ◽  
Esteban Cesar Gabazza ◽  
Tatsuya Hayashi ◽  
Haruhiko Kamada ◽  
Yukihiko Adachi ◽  
...  
Keyword(s):  
Protein C ◽  
Airway Remodeling ◽  
Activated Protein C ◽  
Protective Role

Sepsis and the Role of Activated Protein C

2004 ◽  
Vol 24 (6) ◽  
pp. 40-45
Author(s):  
Janice Tazbir
Keyword(s):  
Protein C ◽  
Activated Protein C

scholarly journals Role of coagulation pathways and treatment with activated protein C in hyperoxic lung injury

Thorax ◽  
2009 ◽  
Vol 64 (2) ◽  
pp. 114-120 ◽  
Author(s):  
M R Looney ◽  
C T Esmon ◽  
M A Matthay
Keyword(s):  
Lung Injury ◽  
Protein C ◽  
Activated Protein C ◽  
Hyperoxic Lung Injury

The structure-function relationship of activated protein C

2011 ◽  
Vol 106 (12) ◽  
pp. 1034-1045. ◽  
Author(s):  
Karin Wildhagen ◽  
Esther Lutgens ◽  
Sarah Loubele ◽  
Hugo ten Cate ◽  
Gerry Nicolaes
Keyword(s):  
Structure Function ◽  
Protein C ◽  
Molecular Mechanisms ◽  
3D Structure ◽  
Activated Protein C ◽  
Protein S ◽  
Coagulation Factors ◽  
Endothelial Protein C Receptor ◽  
Cytoprotective Activity ◽  
Sphingosine 1 Phosphate

SummaryProtein C is the central enzyme of the natural anticoagulant pathway and its activated form APC (activated protein C) is able to proteolyse non-active as well as active coagulation factors V and VIII. Proteolysis renders these cofactors inactive, resulting in an attenuation of thrombin formation and overall down-regulation of coagulation. Presences of the APC cofactor, protein S, thrombomodulin, endothelial protein C receptor and a phospholipid surface are important for the expression of anticoagulant APC activity. Notably, APC also has direct cytoprotective effects on cells: APC is able to protect the endothelial barrier function and expresses anti-inflammatory and anti-apoptotic activities. Exact molecular mechanisms have thus far not been completely described but it has been shown that both the protease activated receptor 1 and EPCR are essential for the cytoprotective activity of APC. Recently it was shown that also other receptors like sphingosine 1 phosphate receptor 1, Cd11b/CD18 and tyrosine kinase with immunoglobulin-like and EGFlike domains 2 are likewise important for APC signalling. Mutagenesis studies are being performed to map the various APC functions and interactions onto its 3D structure and to dissect anticoagulant and cytoprotective properties. The results of these studies have provided a wealth of structure-function information. With this review we describe the state-of-the-art of the intricate structure-function relationships of APC, a protein that harbours several important functions for the maintenance of both humoral and tissue homeostasis.Lessons from natural and engineered mutations

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