Role of the C-terminal tail of the GLUT1 glucose transporter in its expression and function in Xenopus laevis oocytes

Biochemistry ◽  
1995 ◽  
Vol 34 (16) ◽  
pp. 5462-5471 ◽  
Author(s):  
A. Denise Due ◽  
Zhi-chao Qu ◽  
Jean M. Thomas ◽  
Andreas Buchs ◽  
Alvin C. Powers ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Glucose Transporter ◽  
Xenopus Laevis Oocytes ◽  
And Function ◽  
Glut1 Glucose Transporter

scholarly journals Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts

2005 ◽  
Vol 169 (6) ◽  
pp. 859-869 ◽  
Author(s):  
Thomas J. Maresca ◽  
Benjamin S. Freedman ◽  
Rebecca Heald
Keyword(s):  
Xenopus Laevis ◽  
Metaphase Plate ◽  
Histone H1 ◽  
Linker Histone ◽  
Chromosome Architecture ◽  
Linker Histone H1 ◽  
Egg Extracts ◽  
Chromatin Proteins ◽  
And Function

During cell division, condensation and resolution of chromosome arms and the assembly of a functional kinetochore at the centromere of each sister chromatid are essential steps for accurate segregation of the genome by the mitotic spindle, yet the contribution of individual chromatin proteins to these processes is poorly understood. We have investigated the role of embryonic linker histone H1 during mitosis in Xenopus laevis egg extracts. Immunodepletion of histone H1 caused the assembly of aberrant elongated chromosomes that extended off the metaphase plate and outside the perimeter of the spindle. Although functional kinetochores assembled, aligned, and exhibited poleward movement, long and tangled chromosome arms could not be segregated in anaphase. Histone H1 depletion did not significantly affect the recruitment of known structural or functional chromosomal components such as condensins or chromokinesins, suggesting that the loss of H1 affects chromosome architecture directly. Thus, our results indicate that linker histone H1 plays an important role in the structure and function of vertebrate chromosomes in mitosis.

scholarly journals Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes.

1992 ◽  
Vol 12 (9) ◽  
pp. 3776-3783 ◽  
Author(s):  
I Dominguez ◽  
M T Diaz-Meco ◽  
M M Municio ◽  
E Berra ◽  
A García de Herreros ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Growth Factors ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Critical Role ◽  
Study Data ◽  
Xenopus Laevis Oocytes ◽  
Kinase C ◽  
Protein Kinase C Zeta

A number of studies have demonstrated the activation of phospholipase C-mediated hydrolysis of phosphatidylcholine (PC-PLC) both by growth factors and by the product of the ras oncogene, p21ras. Evidence has been presented indicating that the stimulation of this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well as that it is sufficient and necessary for induction of maturation in Xenopus laevis oocytes. However, the mechanism whereby PC-PLC transduces mitogenic signals triggered by growth factors or oncogenes remains to be elucidated. In this study, data are presented that show the involvement of protein kinase C zeta subspecies in the channelling of the mitogenic signal activated by insulin-p21ras-PC-PLC in Xenopus oocytes as well as the lack of a critical role of protein kinase C isotypes alpha, beta, gamma, delta, and epsilon in these pathways.

scholarly journals Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 237
Author(s):  
Armance Gelaude ◽  
Sylvain Slaby ◽  
Katia Cailliau ◽  
Matthieu Marin ◽  
Arlette Lescuyer-Rousseau ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Oocyte Meiosis ◽  
M Phase ◽  
Dependent Inhibition ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Amplification Loop ◽  
Dose Dependent Inhibition

The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

scholarly journals SGK1 increases Na,K-ATP cell-surface expression and function in Xenopus laevis oocytes

2004 ◽  
Vol 448 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Marija Zecevic ◽  
Dirk Heitzmann ◽  
Simone M. R. Camargo ◽  
Francois Verrey
Keyword(s):  
Cell Surface ◽  
Xenopus Laevis ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Xenopus Laevis Oocytes ◽  
And Function

GLUT1 glucose transporter gene transcription is repressed by Sp3. Evidence for a regulatory role of Sp3 during myogenesis 1 1Edited by M. Yaniv

1999 ◽  
Vol 294 (1) ◽  
pp. 103-119 ◽  
Author(s):  
César Fandos ◽  
Manuela Sánchez-Feutrie ◽  
Tomàs Santalucı́a ◽  
Francesc Viñals ◽  
Joan Cadefau ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Glucose Transporter ◽  
Regulatory Role ◽  
Transporter Gene ◽  
Glut1 Glucose Transporter

scholarly journals Local anaesthetics inhibit signalling of human NMDA receptors recombinantly expressed in Xenopus laevis oocytes: role of protein kinase C

2006 ◽  
Vol 96 (1) ◽  
pp. 77-87 ◽  
Author(s):  
K Hahnenkamp ◽  
M.E. Durieux ◽  
A Hahnenkamp ◽  
S.K. Schauerte ◽  
C.W. Hoenemann ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Nmda Receptors ◽  
Local Anaesthetics ◽  
Xenopus Laevis Oocytes ◽  
Kinase C
Sign in / Sign up

Export Citation Format

Share Document