Site-Specific Conjugation of Diethylenetriaminepentaacetic Acid to Recombinant Human Granulocyte-Colony-Stimulating Factor: Preservation of Protein Structure and Function

Biochemistry ◽  
1995 ◽  
Vol 34 (14) ◽  
pp. 4889-4897 ◽  
Author(s):  
Lloyd D. Ralph ◽  
Charles M. Ransone ◽  
Michael Treuheit ◽  
Scott Lauren ◽  
Viswanatham Katta ◽  
...  
Keyword(s):  
Protein Structure ◽  
Structure And Function ◽  
Diethylenetriaminepentaacetic Acid ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Protein Structure And Function ◽  
Site Specific ◽  
And Function ◽  
Stimulating Factor

scholarly journals GlyConnect: a glycan-based conjugation extension of the GlycoDelete technology

2021 ◽  
Author(s):  
Wander Van Breedam ◽  
Karel Thooft ◽  
Francis Santens ◽  
Sandrine Vanmarcke ◽  
Elise Wyseure ◽  
...  
Keyword(s):  
Protein Structure ◽  
Structure And Function ◽  
Protein Backbone ◽  
Protein Structure And Function ◽  
Site Specific ◽  
And Function

Recently, our lab developed GlycoDelete, a technology suite that allows a radical simplification of eukaryotic N-glycosylation. The technology allows to produce glycoproteins that carry single GlcNAc, LacNAc, or LacNAc-Sia type glycans on their N-linked glycosylation sequons. GlycoDelete-type N-glycans are uniquely suited for glycan-based conjugation purposes, as these provide a short, homogeneous and hydrophilic link to the protein backbone. Targeting GlycoDelete-glycans allows for highly site-specific conjugation at sites in the protein which are normally occupied by bulky glycans, thus ensuring minimal interference with protein structure and function. The current manuscript describes the evaluation and optimization of both chemical and chemo-enzymatic conjugation of molecules onto the GlycoDelete-type glycans of a limited set of benchmark proteins

Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

2020 ◽  
Author(s):  
Khondker Rufaka Hossain ◽  
Daniel Clayton ◽  
Sophia C Goodchild ◽  
Alison Rodger ◽  
Richard James Payne ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
Structure And Function ◽  
Membrane Protein Structure ◽  
Protein Structure And Function ◽  
Lipid Environment ◽  
Membrane Pumps ◽  
And Function ◽  
P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

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