Secondary Structure and Orientation of Phospholamban Reconstituted in Supported Bilayers from Polarized Attenuated Total Reflection FTIR Spectroscopy

Biochemistry ◽  
1995 ◽  
Vol 34 (13) ◽  
pp. 4448-4456 ◽  
Author(s):  
Suren A. Tatulian ◽  
Larry R. Jones ◽  
Laxma G. Reddy ◽  
David L. Stokes ◽  
Lukas K. Tamm
Keyword(s):  
Secondary Structure ◽  
Ftir Spectroscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Supported Bilayers

scholarly journals An indirect analytical approach based on ATR-FTIR spectroscopy for determining the FFA content in vegetable oils

RSC Advances ◽  
2020 ◽  
Vol 10 (40) ◽  
pp. 24073-24078
Author(s):  
Yaoyao Dong ◽  
Shaoxia Shi ◽  
Qi Li ◽  
Lingyan Zhang ◽  
Xiuzhu Yu
Keyword(s):  
Fourier Transform ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Ftir Spectroscopy ◽  
Vegetable Oils ◽  
Analytical Approach ◽  
Fourier Transform Infrared ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Novel Approach

In this study, we developed a novel approach for determining a free fatty acid (FFA) in vegetable oils using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy.

In situ attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with non-negative matrix factorization for investigating the synthesis reaction mechanism of 3-amino-4-amino-oxime furazan

2018 ◽  
Vol 10 (48) ◽  
pp. 5817-5822 ◽  
Author(s):  
Tianlong Zhang ◽  
Ting He ◽  
Chunhua Yan ◽  
Xinyu Gao ◽  
Junxiu Ma ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Reaction Mechanism ◽  
Ftir Spectroscopy ◽  
Matrix Factorization ◽  
Fourier Transform Infrared ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Synthesis Reaction ◽  
Non Negative Matrix Factorization

In situ ATR-FTIR combined with non-negative matrix factorization for investigating the synthesis reaction mechanism of 3-amino-4-aminoximefurazan.

scholarly journals Crystallinity of regenerated cellulose from [Bmim]Cl dependent on the hydrogen bond acidity/basicity of anti-solvents

RSC Advances ◽  
2017 ◽  
Vol 7 (65) ◽  
pp. 41004-41010 ◽  
Author(s):  
Zhaosheng Fan ◽  
Jianbo Chen ◽  
Wenji Guo ◽  
Fang Ma ◽  
Suqin Sun ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Molecular Diffusion ◽  
Diffusion Processes ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Regenerated Cellulose ◽  
Hydrogen Bond Acidity

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy was used to monitor the molecular diffusion processes of four anti-solvents in situ.

Two-Dimensional Attenuated Total Reflection/Infrared Correlation Spectroscopy Studies on Concentration and Heat-Induced Structural Changes of Human Serum Albumin in Aqueous Solutions

2002 ◽  
Vol 56 (9) ◽  
pp. 1186-1193 ◽  
Author(s):  
Yuqing Wu ◽  
Koichi Murayama ◽  
Boguslawa Czarnik-Matusewicz ◽  
Yukihiro Ozaki
Keyword(s):  
Secondary Structure ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Ir Spectra ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Correlation Spectroscopy ◽  
Random Coil ◽  
Two Dimensional

Attenuated total reflection (ATR)/FT-IR spectra were measured for human serum albumin (HSA) in aqueous solutions (pH 6.6) with concentrations of 1.0, 2.0, 3.0, 4.0, and 5.0 wt % over a temperature range of 45–80 °C. Generalized two-dimensional (2D) correlation spectroscopy was employed to explore concentration and heat-induced structural variations of HSA in aqueous solutions. To generate 2D correlation spectra, the raw spectra were subjected to the appropriate pretreatment procedure involving ATR correction, subtraction of the spectrum of an aqueous solution, and smoothing. The synchronous and asynchronous correlation spectra were calculated for the concentration-dependent IR spectral variations in the amide I region at various temperatures. The two-dimensional ATR/IR correlation spectra greatly enhance band separation in the region and provide information about the correlation between the amide bands of HSA arising from the same and different secondary structure components. Based on the correlation investigated and previously proposed relationship between the secondary structure elements and the amide band frequencies, we have proposed the detailed assignments in the amide I region at 45 and 80 °C. The proposed assignments are compared with those based on the results of second derivative and Fourier self-deconvolution (FSD) of the ATR/IR spectra. The asynchronous spectrum generated from the concentration-dependent spectral variations at 45 °C show that side chains, the random coil, and extended chains are more sensitive than the α-helices and β-turns to the concentration change. On the other hand, the corresponding spectrum at 80 °C reveals that the conformation changes in side chains and β-turns (or β-strands) of HSA start before those in extended chain, random coil structures, and α-helices.

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