Cleavage by Calicheamicin .gamma.1I of DNA in a Nucleosome Formed on the 5S RNA Gene of Xenopus borealis

Biochemistry ◽  
1995 ◽  
Vol 34 (12) ◽  
pp. 3899-3906 ◽  
Author(s):  
Prasad N. Kuduvalli ◽  
Craig A. Townsend ◽  
Thomas D. Tullius
Keyword(s):  
5S Rna ◽  
Xenopus Borealis

scholarly journals Transition mutations within the Xenopus borealis somatic 5S RNA gene can have independent effects on transcription and TFIIIA binding.

1987 ◽  
Vol 7 (1) ◽  
pp. 486-494 ◽  
Author(s):  
G A McConkey ◽  
D F Bogenhagen
Keyword(s):  
Fine Structure ◽  
Control Region ◽  
Tight Binding ◽  
Sequence Determination ◽  
5S Rna ◽  
Distal Half ◽  
Transcription Efficiency ◽  
Transcription Complexes ◽  
Independent Effects ◽  
Xenopus Borealis

Base-pair changes were introduced into the Xenopus borealis somatic 5S RNA gene by treatment with sodium bisulfite. Mutants were screened by sequence determination. The collection of mutants permitted a detailed investigation of the fine structure of the intragenic control region that binds the transcription factor TFIIIA. Selected mutants were recloned in tandem with a somatic 5S RNA maxigene to permit sensitive measurement of their relative transcription activities. The transcription efficiencies of a number of mutations at the borders of the control region were correlated with TFIIIA binding by using DNase I protection (footprinting) assays. Mutations affecting transcription and TFIIIA binding extended from gene residues 46 to 91. The results reinforce a model in which the distal half of the protected region constitutes a tight binding domain for TFIIIA. A number of transitions in the 5' domain led to significant increases or decreases in transcription efficiency, but resulted in barely detectable changes in TFIIIA binding. Two mutants with C----T transitions at gene residues 52 and 53 were transcribed with increased efficiencies (up phenotype). These results suggest that TFIIIA must make appropriate contacts within the 5' domain of the control region to permit subsequent binding of other factors in stable transcription complexes.

Transition mutations within the Xenopus borealis somatic 5S RNA gene can have independent effects on transcription and TFIIIA binding

1987 ◽  
Vol 7 (1) ◽  
pp. 486-494
Author(s):  
G A McConkey ◽  
D F Bogenhagen
Keyword(s):  
Fine Structure ◽  
Control Region ◽  
Tight Binding ◽  
Sequence Determination ◽  
5S Rna ◽  
Distal Half ◽  
Transcription Efficiency ◽  
Transcription Complexes ◽  
Independent Effects ◽  
Xenopus Borealis

Base-pair changes were introduced into the Xenopus borealis somatic 5S RNA gene by treatment with sodium bisulfite. Mutants were screened by sequence determination. The collection of mutants permitted a detailed investigation of the fine structure of the intragenic control region that binds the transcription factor TFIIIA. Selected mutants were recloned in tandem with a somatic 5S RNA maxigene to permit sensitive measurement of their relative transcription activities. The transcription efficiencies of a number of mutations at the borders of the control region were correlated with TFIIIA binding by using DNase I protection (footprinting) assays. Mutations affecting transcription and TFIIIA binding extended from gene residues 46 to 91. The results reinforce a model in which the distal half of the protected region constitutes a tight binding domain for TFIIIA. A number of transitions in the 5' domain led to significant increases or decreases in transcription efficiency, but resulted in barely detectable changes in TFIIIA binding. Two mutants with C----T transitions at gene residues 52 and 53 were transcribed with increased efficiencies (up phenotype). These results suggest that TFIIIA must make appropriate contacts within the 5' domain of the control region to permit subsequent binding of other factors in stable transcription complexes.

scholarly journals THE DNA OF CAENORHABDITIS ELEGANS

Genetics ◽  
1974 ◽  
Vol 77 (1) ◽  
pp. 95-104
Author(s):  
J E Sulston ◽  
S Brenner
Keyword(s):  
Caenorhabditis Elegans ◽  
Chemical Analysis ◽  
Dna Sequences ◽  
Base Composition ◽  
Nematode Caenorhabditis Elegans ◽  
5S Rna ◽  
Base Pairs ◽  
Small Component ◽  
E Coli ◽  
The Mean

ABSTRACT Chemical analysis and a study of renaturation kinetics show that the nematode, Caenorhabditis elegans, has a haploid DNA content of 8 x IO7 base pairs (20 times the genome of E. coli). Eighty-three percent of the DNA sequences are unique. The mean base composition is 36% GC; a small component, containing the rRNA cistrons, has a base composition of 51% GC. The haploid genome contains about 300 genes for 4s RNA, 110 for 5s RNA, and 55 for (18 + 28)S RNA.

Interrelatedness of 5S RNA sequences investigated by correspondence analysis

1987 ◽  
Vol 24 (3) ◽  
pp. 228-235 ◽  
Author(s):  
Carmen A. Mannella ◽  
Joachim Frank ◽  
Nicholas Delihas
Keyword(s):  
Correspondence Analysis ◽  
5S Rna ◽  
Rna Sequences

Isolation of a gene from Xenopus borealis containing a paired box

1992 ◽  
Vol 20 (1) ◽  
pp. 29S-29S
Author(s):  
JAMES M. FEATHERSTONE ◽  
PHILIP C. TURNER
Keyword(s):  
Xenopus Borealis

A comparison of host-defense peptides in skin secretions of female Xenopus laevis×Xenopus borealis and X. borealis×X. laevis F1 hybrids

Peptides ◽  
2013 ◽  
Vol 45 ◽  
pp. 1-8 ◽  
Author(s):  
Milena Mechkarska ◽  
Manju Prajeep ◽  
Jérôme Leprince ◽  
Hubert Vaudry ◽  
Mohammed A. Meetani ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Host Defense ◽  
F1 Hybrids ◽  
Host Defense Peptides ◽  
Skin Secretions ◽  
Defense Peptides ◽  
Xenopus Borealis
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