Kinetics of Transport of Dialkyloxacarbocyanines in Multidrug-Resistant Cell Lines Overexpressing P-glycoprotein: Interrelationship of Dye Alkyl Chain Length, Cellular Flux, and Drug Resistance

Biochemistry ◽  
1995 ◽  
Vol 34 (11) ◽  
pp. 3858-3872 ◽  
Author(s):  
Randy M. Wadkins ◽  
Peter J. Houghton
Keyword(s):  
Drug Resistance ◽  
Chain Length ◽  
Cell Lines ◽  
Alkyl Chain ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Alkyl Chain Length ◽  
P Glycoprotein ◽  
Kinetics Of

Probing daunorubicin accumulation defects in non-P-glycoprotein expressing multidrug-resistant cell lines using digitonin

1992 ◽  
Vol 50 (6) ◽  
pp. 906-911 ◽  
Author(s):  
C. H. M. Versantvoort ◽  
H. J. Broxterman ◽  
N. Feller ◽  
H. Dekker ◽  
C. M. Kuiper ◽  
...  
Keyword(s):  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
P Glycoprotein

Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodies

Nature ◽  
1985 ◽  
Vol 316 (6031) ◽  
pp. 820-823 ◽  
Author(s):  
Norbert Kartner ◽  
Deanna Evernden-Porelle ◽  
Grace Bradley ◽  
Victor Ling
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
P Glycoprotein

scholarly journals Structure and expression of the human MDR (P-glycoprotein) gene family.

1989 ◽  
Vol 9 (9) ◽  
pp. 3808-3820 ◽  
Author(s):  
J E Chin ◽  
R Soffir ◽  
K E Noonan ◽  
K Choi ◽  
I B Roninson
Keyword(s):  
Gene Family ◽  
Cell Lines ◽  
Efflux Pump ◽  
Expression Patterns ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Mdr1 Gene ◽  
Glycoprotein Gene ◽  
P Glycoprotein ◽  
Mdr Genes

The human MDR (P-glycoprotein) gene family is known to include two members, MDR1 and MDR2. The product of the MDR1 gene, which is responsible for resistance to different cytotoxic drugs (multidrug resistance), appears to serve as an energy-dependent efflux pump for various lipophilic compounds. The function of the MDR2 gene remains unknown. We have examined the structure of the human MDR gene family by Southern hybridization of DNA from different multidrug-resistant cell lines with subfragments of MDR1 cDNA and by cloning and sequencing of genomic fragments. We have found no evidence for any other cross-hybridizing MDR genes. The sequence of two exons of the MDR2 gene was determined from genomic clones. Hybridization with single-exon probes showed that the human MDR1 gene is closely related to two genes in mouse and hamster DNA, whereas MDR2 corresponds to one rodent gene. The human MDR locus was mapped by field-inversion gel electrophoresis, and both MDR genes were found to be linked within 330 kilobases. The expression patterns of the human MDR genes were examined by enzymatic amplification of cDNA. In multidrug-resistant cell lines, increased expression of MDR1 mRNA was paralleled by a smaller increase in the levels of MDR2 mRNA. In normal human tissues, MDR2 was coexpressed with MDR1 in the liver, kidney, adrenal gland, and spleen. MDR1 expression was also detected in colon, lung, stomach, esophagus, muscle, breast, and bladder.

scholarly journals Spontaneous Reversal of P-Glycoprotein Expression in Multidrug Resistant Cell Lines*

2003 ◽  
Vol 93 (6) ◽  
pp. 297-304 ◽  
Author(s):  
Henrik Green ◽  
Kourosh Lotfi ◽  
Anna-Lena Zackrisson ◽  
Curt Peterson
Keyword(s):  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
P Glycoprotein ◽  
Spontaneous Reversal

Subcellular immunolocalization of P-glycoprotein in multidrug resistant cell lines

1994 ◽  
Vol 5 ◽  
pp. 13
Author(s):  
K Scotlandi ◽  
N Baldini ◽  
M Serra ◽  
N Zini ◽  
M C Manara ◽  
...  
Keyword(s):  
Cell Lines ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
P Glycoprotein
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