scholarly journals Lamellarin D Bioconjugates II: Synthesis and Cellular Internalization of Dendrimer and Nuclear Location Signal Derivatives

2009 ◽  
Vol 20 (6) ◽  
pp. 1112-1121 ◽  
Author(s):  
Daniel Pla ◽  
Marc Martí ◽  
Josep Farrera-Sinfreu ◽  
Daniel Pulido ◽  
Andrés Francesch ◽  
...  
Keyword(s):  
Cellular Internalization ◽  
Nuclear Location Signal ◽  
Nuclear Location

Escherichia coli RecA protein modified with a nuclear location signal binds to chromosomes in living mammalian cells

1992 ◽  
Vol 198 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Mitsuru Kido ◽  
Yoshihiro Yoneda ◽  
Mahito Nakanishi ◽  
Tsuyoshi Uchida ◽  
Yoshio Okada
Keyword(s):  
Escherichia Coli ◽  
Mammalian Cells ◽  
Reca Protein ◽  
Nuclear Location Signal ◽  
Nuclear Location

Targeting of T7 RNA polymerase to tobacco nuclei mediated by an SV40 nuclear location signal

1991 ◽  
Vol 17 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Michael W. Lassner ◽  
Aubrey Jones ◽  
Steve Daubert ◽  
Luca Comai
Keyword(s):  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Nuclear Location Signal ◽  
Nuclear Location

The effect of protein context on nuclear location signal function

Cell ◽  
1987 ◽  
Vol 50 (3) ◽  
pp. 465-475 ◽  
Author(s):  
Bruce L. Roberts ◽  
William D. Richardson ◽  
Alan E. Smith
Keyword(s):  
Signal Function ◽  
Nuclear Location Signal ◽  
Nuclear Location

Intramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-κB subunit

Cell ◽  
1992 ◽  
Vol 68 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
Thomas Henkel ◽  
Ulrike Zabel ◽  
Karen van Zee ◽  
Judith M. Müller ◽  
Ellen Fanning ◽  
...  
Keyword(s):  
Dimerization Domain ◽  
Nuclear Location Signal ◽  
Nuclear Location

Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen

1986 ◽  
Vol 6 (11) ◽  
pp. 4136-4139
Author(s):  
W H Colledge ◽  
W D Richardson ◽  
M D Edge ◽  
A E Smith
Keyword(s):  
Dna Replication ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Site Directed Mutagenesis ◽  
Large T Antigen ◽  
Basic Residue ◽  
Nuclear Location Signal ◽  
Nuclear Location ◽  
Low Levels

Site-directed mutagenesis was used to change Lys-128 of the simian virus 40 large-T nuclear location signal to Met, Ile, Arg, Gln, Asn, Leu, or His. Except for the large-T antigen of the Arg mutation, which was present in cytoplasmic and nuclear compartments, the resultant proteins were unable to enter the nucleus. By contrast, mutations at other sites within the signal were generally less severe in their effect. In some cases (Lys-128 to Gln, Asn, and His), the apparently cytoplasmic variants were able to support limited plasmid DNA replication, suggesting that low levels of large-T antigen undetectable by immunofluorescence were present in the nucleus. Such mutants did not support viral DNA replication. We conclude that there is a strong requirement for a basic residue at position 128 in the large-T nuclear location signal, with Lys the preferred residue.

scholarly journals Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen.

1986 ◽  
Vol 6 (11) ◽  
pp. 4136-4139 ◽  
Author(s):  
W H Colledge ◽  
W D Richardson ◽  
M D Edge ◽  
A E Smith
Keyword(s):  
Dna Replication ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Site Directed Mutagenesis ◽  
Large T Antigen ◽  
Basic Residue ◽  
Nuclear Location Signal ◽  
Nuclear Location ◽  
Low Levels

Site-directed mutagenesis was used to change Lys-128 of the simian virus 40 large-T nuclear location signal to Met, Ile, Arg, Gln, Asn, Leu, or His. Except for the large-T antigen of the Arg mutation, which was present in cytoplasmic and nuclear compartments, the resultant proteins were unable to enter the nucleus. By contrast, mutations at other sites within the signal were generally less severe in their effect. In some cases (Lys-128 to Gln, Asn, and His), the apparently cytoplasmic variants were able to support limited plasmid DNA replication, suggesting that low levels of large-T antigen undetectable by immunofluorescence were present in the nucleus. Such mutants did not support viral DNA replication. We conclude that there is a strong requirement for a basic residue at position 128 in the large-T nuclear location signal, with Lys the preferred residue.

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