Solid-Phase Synthesis of Oligonucleotide Conjugates Useful for Delivery and Targeting of Potential Nucleic Acid Therapeutics

2009 ◽  
Vol 20 (6) ◽  
pp. 1065-1094 ◽  
Author(s):  
Harri Lönnberg
Keyword(s):  
Nucleic Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Nucleic Acid Therapeutics ◽  
Oligonucleotide Conjugates

Solid-Phase Synthesis of Glyco-Oligonucleotide Conjugates

Synlett ◽  
2001 ◽  
Vol 2001 (06) ◽  
pp. 0745-0748 ◽  
Author(s):  
Matteo Adinolfi ◽  
Lorenzo De Napoli ◽  
Giovanni Di Fabio ◽  
Luigi Guariniello ◽  
Alfonso Iadonisi ◽  
...  
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Oligonucleotide Conjugates

Solid-Phase Synthesis of DNA Chemical Sensor

2013 ◽  
Vol 815 ◽  
pp. 305-311 ◽  
Author(s):  
Hui Yong Zhang
Keyword(s):  
Nucleic Acid ◽  
Chemical Sensor ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Acid Synthesis ◽  
Synthetic Approach ◽  
Biological Interactions ◽  
Oligonucleotide Synthesis ◽  
Phase Synthesis ◽  
Essential Components

Oligonucleotides are essential components of many applications in molecular biology. The synthesis chemistry is robust and commercial oligonucleotide synthesizers have taken advantage of the chemistry to provide oligonucleotides of high quality and purity. This paper established nucleic acid synthesis platform to carry out the synthesis of the labeled nucleic acid probes based on the DNA synthesizer and solid-phase synthesis technology. We chose to study the automated synthesis starting from DMT protected FAM labeled amidite attached to controlled pore glass (CPG) support and the standard trityl-off oligonucleotide synthesis cycle was performed, yielding the solid-supported oligonucleotide. The reported automated solid-phase oligonucleotide synthesis procedure successfully employs the common iterative synthesis, deblocking, activation, coupling, capping, oxidation, and isolation steps in standard oligonucleotide synthesis. The automated synthetic approach can also be applied to oligonucleotides of different length, composition of nucleotide, demonstrating the universality of the method. Moreover, the synthesis involved the use of commercially available, safe, stable, and inexpensive reagents, particularly advantageous and attractive for their use in automated solid-phase synthesis. The synthesis allows custom tailoring of their structure to the requirements of biological assays within hours, as opposed to traditional approaches that require weeks or months of work in the laboratory. Therefore it will become much easier to investigate biological interactions and optimize for objectives such as the receptor mediated targeting of oligonucleotides.

Solid-phase synthesis of arginine-based double-tailed cationic lipopeptides: potent nucleic acid carriers

2011 ◽  
Vol 47 (48) ◽  
pp. 12774 ◽  
Author(s):  
Aleksandra Liberska ◽  
Annamaria Lilienkampf ◽  
Asier Unciti-Broceta ◽  
Mark Bradley
Keyword(s):  
Nucleic Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis

Solid-Phase Synthesis of Peptide-Derived Enantiospecific Nucleic Acid Analogs

1996 ◽  
Vol 24 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Vibhakar J. Shah ◽  
Robert Cerpa ◽  
Irwin D. Kuntz, Jr. ◽  
George L. Kenyon
Keyword(s):  
Nucleic Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Nucleic Acid Analogs

Modular Nucleic Acid Surrogates. Solid Phase Synthesis of α-Helical Peptide Nucleic Acids (αPNAs)

1999 ◽  
Vol 1 (3) ◽  
pp. 403-406 ◽  
Author(s):  
Philip Garner ◽  
Subhakar Dey ◽  
Yumei Huang ◽  
Xiao Zhang
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Peptide Nucleic Acids ◽  
Phase Synthesis ◽  
Helical Peptide

An Efficient, Convenient Solid-Phase Synthesis of Amino Acid-Modified Peptide Nucleic Acid Monomers and Oligomers

2006 ◽  
Vol 17 (2) ◽  
pp. 551-558 ◽  
Author(s):  
Baghavathy S. Balaji ◽  
Fabio Gallazzi ◽  
Fang Jia ◽  
Michael R. Lewis
Keyword(s):  
Amino Acid ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis
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