Fluorine-18 Click Radiosynthesis and Preclinical Evaluation of a New18F-Labeled Folic Acid Derivative

2008 ◽  
Vol 19 (12) ◽  
pp. 2462-2470 ◽  
Author(s):  
Tobias L. Ross ◽  
Michael Honer ◽  
Phoebe Y. H. Lam ◽  
Thomas L. Mindt ◽  
Viola Groehn ◽  
...  
Keyword(s):  
Folic Acid ◽  
Acid Derivative ◽  
Preclinical Evaluation

Radiosynthesis and in vitro evaluation of 99mTc(CO)3-labeled folic acid derivative

2011 ◽  
Vol 290 (1) ◽  
pp. 89-93 ◽  
Author(s):  
Drishty Satpati ◽  
Archana Mukherjee ◽  
Meera Venkatesh ◽  
Sharmila Banerjee
Keyword(s):  
Folic Acid ◽  
Acid Derivative ◽  
In Vitro Evaluation

Strontium folic acid derivative functionalized titanium surfaces for enhanced osteogenic differentiation of mesenchymal stem cells in vitro and bone formation in vivo

2017 ◽  
Vol 5 (33) ◽  
pp. 6811-6826 ◽  
Author(s):  
Kui Xu ◽  
Weizhen Chen ◽  
Caiyun Mu ◽  
Yonglin Yu ◽  
Kaiyong Cai
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Folic Acid ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Acid Derivative ◽  
Titanium Surfaces

Strontium folic acid derivative (FASr) functionalized titanium surfaces improve the in vitro osteogenic differentiation of MSCs and osseointegration in vivo.

Collaborative Study of Microbiological Assay for Folic Acid in Food

1964 ◽  
Vol 47 (4) ◽  
pp. 765-771
Author(s):  
Laura M Flynn
Keyword(s):  
Folic Acid ◽  
Acid Derivative ◽  
Phosphate Buffer ◽  
Collaborative Study ◽  
Microbiological Assay ◽  
Extraction Procedures

Abstract The extraction and microbiological determination of folic acid in foods was studied collaboratively. Four samples and four extraction procedures were used. Extracts were assayed for folic acid activity with L. casei and S. faecalis, and chick assays were also made on the samples. The four extraction procedures were: (A) Incubating 4 hours with chick pancreas at 37 °C after autoclaving 15 min. at 15 lb in 0.2M pH 7.2 phosphate buffer and cooling; (B) autoclaving 30 min. at 15 lb in potassium ascorbate (pH 6.0, 6 mg/ml); (C) incubating 4 hours with chick pancreas enzyme after autoclaving 15 min. at 15 lb in 0.2M pH 8 phosphate buffer, cooling, and adding potassium ascorbate (20 mg/ml, pH 6) in volume equal to the phosphate buffer; and (D) autolyzing in incubator 4 hours at 37 °C in mixture of equal volumes of potassium ascorbate (20 mg/ml, pH 6) and 0.2M phosphate buffer, pH 8.0. The highest values were obtained with L. casei in extracts containing potassium ascorbate. A rough estimation of the types of folic acid derivative in each extract could be made by comparing values obtained in the various extracts with each organism. Collaborators’ results by chick assay agreed fairly well with those by microbiological assay, but further work is needed to standardize the extractions and both types of assays.

Cytotoxicity and Cancer Detection Ability of the Luminescent Nanoporous Silica Spheres Immobilized with Folic Acid Derivative

2012 ◽  
Vol 529-530 ◽  
pp. 630-635 ◽  
Author(s):  
Motohiro Tagaya ◽  
Nobutaka Hanagata ◽  
Toshiyuki Ikoma ◽  
Takaomi Kobayashi ◽  
Kota Shiba ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cancer Cells ◽  
Acid Derivative ◽  
Cancer Detection ◽  
Culture Medium ◽  
Fluorescent Microscopy ◽  
Nanoporous Silica ◽  
Silica Spheres ◽  
Characteristic Peak ◽  
Intense Luminescence

Luminescent europium(III)-doped nanoporous silica spheres (Eu:NPS) were synthesized, and folic acid derivative (FA: folate N-hydroxysuccinimidyl ester) as targeting ligand for cancer cells was immobilized on the sphere through a mediation of 3-aminopropyltriethoxysilane (APTES). The ordered nanopores were preserved with the immobilization. The surface area decreased only with the APTES immobilization, suggesting that the FA was immobilized only on the outer surface of the nanopores. The photoluminescence of the spheres showed the characteristic peak due to interactions between the FA and Eu3+, and further the luminescence can be clearly detected by a fluorescent microscopy. The spheres were highly dispersed stability in cell culture medium to exhibit the nontoxic in the proliferation stage of Hela cancer cells and NIH3T3 fibroblasts, and specifically bindto the Hela cells. The binding and uptake spheres showed the intense luminescence. Thus, the luminescent FA-immobilized Eu:NPS spheres will exhibit the targeting and imaging abilities for cancer cells.

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