scholarly journals Cellular Uptake and Intracellular Trafficking of Antisense and siRNA Oligonucleotides

2011 ◽  
Vol 23 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Rudolph L. Juliano ◽  
Xin Ming ◽  
Osamu Nakagawa
Keyword(s):  
Cellular Uptake ◽  
Intracellular Trafficking

Cellular Uptake, Intracellular Trafficking, and Stability of Biocompatible Metal-Organic Framework (MOF) Particles in Kupffer Cells

2019 ◽  
Vol 16 (6) ◽  
pp. 2315-2325 ◽  
Author(s):  
Mikhail Durymanov ◽  
Anastasia Permyakova ◽  
Saad Sene ◽  
Ailin Guo ◽  
Christian Kroll ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Kupffer Cells ◽  
Intracellular Trafficking ◽  
Metal Organic Framework ◽  
Metal Organic ◽  
Organic Framework

scholarly journals Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Yaguang Zhang ◽  
Hongmei Shu ◽  
Jing Hu ◽  
Min Zhang ◽  
Junweng Wu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Transfection Efficiency ◽  
Cross Linking ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Dna Complexes ◽  
Dynamic Source

A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake. The binding assessment of R13 toαvβ3 positive cells was performed by HRP labeling. The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency. The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency. The results indicated that the bifunctional peptide R13 had the ability of binding toαvβ3 positive cellsin vitro. The modification of P123-PEI-R13 with R13 made it display new property of internalization. P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route. After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.

Elucidation of cellular uptake and intracellular trafficking of heparosan polysaccharide-based micelles in various cancer cells

2019 ◽  
Vol 130 ◽  
pp. 755-764 ◽  
Author(s):  
Lipeng Qiu ◽  
Xiaotian Shan ◽  
Miaomiao Long ◽  
Kamel S. Ahmed ◽  
Li Zhao ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cellular Uptake ◽  
Intracellular Trafficking

Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy

2010 ◽  
Vol 111 (2) ◽  
pp. 488-496 ◽  
Author(s):  
Tian Tian ◽  
Yuanyuan Wang ◽  
Haitao Wang ◽  
Zhaoqi Zhu ◽  
Zhongdang Xiao
Keyword(s):  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Live Cell ◽  
Live Cell Microscopy ◽  
Cell Microscopy

scholarly journals Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

2018 ◽  
Vol 20 (1) ◽  
pp. 231-242 ◽  
Author(s):  
Claudia Battistella ◽  
Romain Guiet ◽  
Olivier Burri ◽  
Arne Seitz ◽  
Stéphane Escrig ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Hydroxypropyl Methacrylamide

scholarly journals Cellular uptake of a cystine-knot peptide and modulation of its intracellular trafficking

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xinxin Gao ◽  
Karen Stanger ◽  
Harini Kaluarachchi ◽  
Till Maurer ◽  
Paulina Ciepla ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Cystine Knot

scholarly journals Effect of Polyplex Morphology on Cellular Uptake, Intracellular Trafficking, and Transgene Expression

ACS Nano ◽  
2013 ◽  
Vol 7 (12) ◽  
pp. 10612-10620 ◽  
Author(s):  
Julie Shi ◽  
Jennifer L. Choi ◽  
Brian Chou ◽  
Russell N. Johnson ◽  
Joan G. Schellinger ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Transgene Expression
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