Engineering Bioactive Surfaces with Fischer Carbene Complex: Protein A on Self-Assembled Monolayer for Antibody Sensing

2011 ◽  
Vol 22 (6) ◽  
pp. 1202-1209 ◽  
Author(s):  
Piyali Dutta ◽  
Sudeshna Sawoo ◽  
Namrata Ray ◽  
Othman Bouloussa ◽  
Amitabha Sarkar
Keyword(s):  
Protein A ◽  
Self Assembled Monolayer ◽  
Carbene Complex ◽  
Fischer Carbene ◽  
Complex Protein ◽  
Bioactive Surfaces ◽  
Self Assembled

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

2019 ◽  
Author(s):  
Luke Clifton ◽  
Nicoló Paracini ◽  
Arwel V. Hughes ◽  
Jeremy H. Lakey ◽  
Nina-Juliane Seinke ◽  
...  
Keyword(s):  
Fluid Phase ◽  
Interlayer Thickness ◽  
Supported Bilayers ◽  
Self Assembled Monolayer ◽  
High Coverage ◽  
Nano Technology ◽  
Surface Distance ◽  
Potential Applications ◽  
Self Assembled ◽  
Coated Surfaces

<p>We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.</p><br>

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