scholarly journals Photocleavable Peptide-Conjugated Magnetic Beads for Protein Kinase Assays by MALDI-TOF MS

2010 ◽  
Vol 21 (10) ◽  
pp. 1917-1924 ◽  
Author(s):  
Guangchang Zhou ◽  
Xiaoliang Yan ◽  
Ding Wu ◽  
Stephen J. Kron
Keyword(s):  
Protein Kinase ◽  
Magnetic Beads ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

MALDI-TOF MS and Magnetic Beads for Rapid Seafood Allergen Tests

2021 ◽  
Author(s):  
Xin Zhao ◽  
Jiayin Lu ◽  
Shuping Long ◽  
Winnie C. Soko ◽  
Qin Qin ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Discovery of serum protein biomarkers in rheumatoid arthritis using MALDI-TOF-MS combined with magnetic beads

2011 ◽  
Vol 12 (3) ◽  
pp. 145-151 ◽  
Author(s):  
Xiaoxue Zhang ◽  
Zhaolin Yuan ◽  
Bo Shen ◽  
Min Zhu ◽  
Chibo Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Protein ◽  
Magnetic Beads ◽  
Protein Biomarkers ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Serum Proteome Profiling Detects Myelodysplastic Syndromes and Different Expression of Fibrinogen Between RAEB and RCMD

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5082-5082
Author(s):  
Liye Zhong ◽  
TianHao Liu ◽  
SuXia Geng ◽  
Zesheng Lu ◽  
Jianyu Weng ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Predictive Models ◽  
Healthy Subjects ◽  
Magnetic Beads ◽  
Hematologic Malignancies ◽  
Clinical Classification ◽  
Maldi Tof Ms ◽  
Serum Proteome ◽  
Maldi Tof ◽  
Tof Ms

Abstract BACKGROUND&OBJECTIVE FMyelodysplastic syndromes (MDS) are among the most frequent hematologic malignancies. The diagnosis of MDS can be difficult, and there is a paucity of molecular markers. The pathophysiology is still largely unknown. Therefore, we investigated whether serum proteome profiling may serve as a noninvasive platform to discover novel molecular markers for MDS and establish the predictive models that may be of help to serologic diagnosis and classification of MDS. METHODS FSerum samples were collected from 14 MDS patients including to 8 Refractory anemia with excess blasts in transformation (RAEB) and 6 Refractory cytopenia with multilineage dysplasia (RCMD) and 18 non-MDS hematologic malignancies and 8 age- and sex-matched healthy subjects. Serum peptides were separated and purified with a purification kit of magnetic beads, using magnetic beads-based weak cation exchange chromatography (MB-WCX) and MB-IMAC Cu, bases on immobilized metal ion affinity chromatography on the surface of superparamagnetic microparticles. We generated serum proteome profiles by matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF- MS) and identified a profile that distinguishes MDS from non-MDS hematologic malignancies and healthy subjects. RESULTS FA totaI of 146 effective protein peaks were detected at the molecular range of 1.02 tO 10.25 ku, Among which 7 protein peaks were different significantly among MDS patients, non-MDS hematologic malignancies and healthy subjects (P<0.05). There was also different for Peptide mass fingerprinting in MDS patients, and the samples were divided into two groups, which was identical with clinical classification about RAEB and RCMD, using 3-cross validation approach. There was significantly different expression protein between RCMD and RAEB patients, which was identified as a piece of fibrinogen peptide. The expressions of fibrinogen in RAEB subtype patients were higher than RCMD subtype patients. CONCLUSION F Using the MALDI-TOF-MS technique may help to identify serum proteomic biomarkers related to MDS. The predictive models can discriminate MDS patients from other hematologic malignancies and healthy people effectively and help to identify MDS clinical classification. The different expression of Fibrinogen between RAEB and RCMD may suggested heterogeneity of etiopathogenisis in different subtypeof MDS.

scholarly journals MALDI-TOF mass spectrometry-based quantification of C-peptide in diabetes patients

2019 ◽  
Vol 26 (1) ◽  
pp. 55-62
Author(s):  
MeiHua Wan ◽  
Yichao Wang ◽  
Lingpeng Zhan ◽  
Jia Fan ◽  
Tony Y Hu
Keyword(s):  
Mass Spectrometry ◽  
Magnetic Beads ◽  
Cell Function ◽  
Internal Standard ◽  
Reference Method ◽  
C Peptide ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Limit Of Quantitation ◽  
Tof Ms

Background Serum C-peptide concentrations reflect insulin secretion and beta cell function and can be used to diagnose and distinguish type-1 and type-2 diabetes. C-peptide is a more accurate indicator of insulin status than direct insulin measurement for monitoring patients with diabetes. However, the current methods available for C-peptide quantification exhibit poor reproducibility, are costly, and require highly trained laboratory personnel. Here, we have developed and evaluated a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assay to standardize C-peptide measurements, providing highly accurate and comparable results across testing systems and laboratories. Methods C-peptide from human serum was enriched using antibody-conjugated magnetic beads. The eluted isolates were further modified with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) to enhance the ionization of naturally acidic C-peptide. After desalting with ZipTips, the samples were subjected to MALDI-TOF MS analysis. Recombinant human C-peptide was used to develop the assay, and a heavy isotope labeled human C-peptide was used as an internal standard for quantification. Results The MALDI-TOF MS method was validated in accordance with the restrictions of the device, with a limit of quantitation of 25 pmol/L. A correlation between the MAL-DI-TOF MS assay and a reference method was conducted using patient samples. The resulting regression revealed good agreement. Conclusions A simple, high-throughput, cost effective and quantitative MALDI-TOF MS C-peptide assay has been successfully developed and validated in clinical serum samples.

Identification of serum biomarkers for ovarian cancer using MALDI–TOF-MS combined with magnetic beads

2011 ◽  
Vol 17 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Shengjun Wu ◽  
Kai Xu ◽  
Guang Chen ◽  
Jun Zhang ◽  
Zhiwei Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Magnetic Beads ◽  
Serum Biomarkers ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Diagnosis of colorectal cancer by MALDI-TOF-MS combined with WCX magnetic beads

2012 ◽  
Vol 20 (20) ◽  
pp. 1900
Author(s):  
Xiu-Li Wang ◽  
Chun-Fang Gao ◽  
Nai-Jun Fan
Keyword(s):  
Colorectal Cancer ◽  
Magnetic Beads ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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