scholarly journals Labeling Live Cells by Copper-Catalyzed Alkyne−Azide Click Chemistry

2010 ◽  
Vol 21 (10) ◽  
pp. 1912-1916 ◽  
Author(s):  
Vu Hong ◽  
Nicole F. Steinmetz ◽  
Marianne Manchester ◽  
M. G. Finn
Keyword(s):  
Click Chemistry ◽  
Live Cells

5-Ethynylcytidine as a new agent for detecting RNA synthesis in live cells by “click” chemistry

2013 ◽  
Vol 434 (1) ◽  
pp. 128-135 ◽  
Author(s):  
Dezhong Qu ◽  
Li Zhou ◽  
Wei Wang ◽  
Zhe Wang ◽  
Guoxin Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Rna Synthesis ◽  
Live Cells

scholarly journals Supported core–shell nanobiosensors for quantitative fluorescence imaging of extracellular pH

2014 ◽  
Vol 50 (89) ◽  
pp. 13746-13749 ◽  
Author(s):  
Jérémie Asselin ◽  
Carl Roy ◽  
Denis Boudreau ◽  
Younès Messaddeq ◽  
Rihab Bouchareb ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Ph Sensitive ◽  
Extracellular Ph ◽  
Live Cells ◽  
Core Shell ◽  
Spatially Resolved ◽  
Quantitative Fluorescence ◽  
Silica Substrates

“Click” chemistry was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent ion-sensitive surfaces for quantitative and spatially-resolved extracellular measurements on live cells.

Two-Color Glycan Labeling of Live Cells by a Combination of Diels-Alder and Click Chemistry

2013 ◽  
Vol 52 (15) ◽  
pp. 4265-4268 ◽  
Author(s):  
Andrea Niederwieser ◽  
Anne-Katrin Späte ◽  
Long Duc Nguyen ◽  
Christian Jüngst ◽  
Werner Reutter ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Diels Alder ◽  
Live Cells

scholarly journals Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells

RSC Advances ◽  
2021 ◽  
Vol 11 (33) ◽  
pp. 20335-20341
Author(s):  
Louis Allott ◽  
Cen Chen ◽  
Marta Braga ◽  
Sau Fung Jacob Leung ◽  
Ning Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Live Cells

Bioorthogonal IEDDA “click” can interrogate intracellular hypoxia using a radioactive reporter molecule.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

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