Enhanced Circulating Half-Life and Antitumor Activity of a Site-Specific Pegylated Interferon-α Protein Therapeutic

2008 ◽  
Vol 19 (1) ◽  
pp. 299-305 ◽  
Author(s):  
Stacie J. Bell ◽  
Christine M. Fam ◽  
Elizabeth A. Chlipala ◽  
Sharon J. Carlson ◽  
Ji I. Lee ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Half Life ◽  
Pegylated Interferon ◽  
Interferon Α ◽  
Site Specific ◽  
Protein Therapeutic ◽  
A Site

Polymerization Induced Self-Assembly of a Site-Specific Interferon α-Block Copolymer Conjugate into Micelles with Remarkably Enhanced Pharmacology

2018 ◽  
Vol 140 (33) ◽  
pp. 10435-10438 ◽  
Author(s):  
Xinyu Liu ◽  
Mengmeng Sun ◽  
Jiawei Sun ◽  
Jin Hu ◽  
Zhuoran Wang ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Self Assembly ◽  
Interferon Α ◽  
Site Specific ◽  
A Site

scholarly journals Site-Specific PEGylation of Anti-Mesothelin Recombinant Immunotoxins Increases Half-life and Antitumor Activity

2019 ◽  
Vol 19 (3) ◽  
pp. 812-821 ◽  
Author(s):  
Zeliang Zheng ◽  
Ryuhei Okada ◽  
Hisataka Kobayashi ◽  
Tadanobu Nagaya ◽  
Junxia Wei ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Half Life ◽  
Site Specific ◽  
Recombinant Immunotoxins

scholarly journals Site-Specific Lipidation of a Small-Sized Protein Binder Enhances the Antitumor Activity through Extended Blood Half-Life

ACS Omega ◽  
2020 ◽  
Vol 5 (31) ◽  
pp. 19778-19784
Author(s):  
Tae Yoon Kim ◽  
You Ree Nam ◽  
Jin Ho Park ◽  
Dong-Eun Lee ◽  
Hak-Sung Kim
Keyword(s):  
Antitumor Activity ◽  
Half Life ◽  
Site Specific

scholarly journals Functional expression of a yeast mitochondrial intron-encoded protein requires RNA processing at a conserved dodecamer sequence at the 3' end of the gene.

1989 ◽  
Vol 9 (4) ◽  
pp. 1507-1512 ◽  
Author(s):  
H Zhu ◽  
H Conrad-Webb ◽  
X S Liao ◽  
P S Perlman ◽  
R A Butow
Keyword(s):  
Genetic Analysis ◽  
Rna Processing ◽  
Fold Increase ◽  
Functional Expression ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Site Specific ◽  
Var1 Gene ◽  
A Site

All mRNAs of yeast mitochondria are processed at their 3' ends within a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3'. A dominant nuclear suppressor, SUV3-I, was previously isolated because it suppresses a dodecamer deletion at the 3' end of the var1 gene. We have tested the effects of SUV3-1 on a mutant containing two adjacent transversions within a dodecamer at the 3' end of fit1, a gene located within the 1,143-base-pair intron of the 21S rRNA gene, whose product is a site-specific endonuclease required in crosses for the quantitative transmission of that intron to 21S alleles that lack it. The fit1 dodecamer mutations blocked both intron transmission and dodecamer cleavage, neither of which was suppressed by SUV3-1 when present in heterozygous or homozygous configurations. Unexpectedly, we found that SUV3-1 completely blocked cleavage of the wild-type fit1 dodecamer and, in SUV3-1 homozygous crosses, intron conversion. In addition, SUV3-1 resulted in at least a 40-fold increase in the amount of excised intron accumulated. Genetic analysis showed that these phenotypes resulted from the same mutation. We conclude that cleavage of a wild-type dodecamer sequence at the 3' end of the fit1 gene is essential for fit1 expression.

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