Folate-Conjugated Iron Oxide Nanoparticles for Solid Tumor Targeting as Potential Specific Magnetic Hyperthermia Mediators: Synthesis, Physicochemical Characterization, and in Vitro Experiments

2005 ◽  
Vol 16 (5) ◽  
pp. 1181-1188 ◽  
Author(s):  
Fabio Sonvico ◽  
Stéphane Mornet ◽  
Sébastien Vasseur ◽  
Catherine Dubernet ◽  
Danielle Jaillard ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Solid Tumor ◽  
Tumor Targeting ◽  
Physicochemical Characterization ◽  
Magnetic Hyperthermia ◽  
Oxide Nanoparticles ◽  
In Vitro Experiments

Potential toxic effects of iron oxide nanoparticles in in vivo and in vitro experiments

2011 ◽  
Vol 32 (6) ◽  
pp. 446-453 ◽  
Author(s):  
Brigitta Szalay ◽  
Erzsébet Tátrai ◽  
Gábor Nyírő ◽  
Tünde Vezér ◽  
Gyula Dura
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Toxic Effects ◽  
Oxide Nanoparticles ◽  
In Vitro Experiments

scholarly journals Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

2019 ◽  
Vol 10 ◽  
pp. 1860-1872 ◽  
Author(s):  
Xianping Liu ◽  
Chengjuan Du ◽  
Haichun Li ◽  
Ting Jiang ◽  
Zimiao Luo ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Optical Imaging ◽  
Iron Oxide Nanoparticles ◽  
Tumor Targeting ◽  
Tumor Resection ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Bimodal Imaging

In this work, a peptide-modified, biodegradable, nontoxic, brain-tumor-targeting nanoprobe based on superparamagnetic iron oxide nanoparticles (SPIONs) (which have been commonly used as T 2-weighted magnetic resonance (MR) contrast agents) was successfully synthesized and applied for accurate molecular MR imaging and sensitive optical imaging. PEPHC1, a short peptide which can specifically bind to epidermal growth factor receptor variant III (EGFRvIII) that is overexpressed in glioblastoma, was conjugated with SPIONs to construct the nanoprobe. Both in vitro and in vivo MR and optical imaging demonstrated that the as-constructed nanoprobe was effective and sensitive for tumor targeting with desirable biosafety. Given its desirable properties such as a 100 nm diameter (capable of penetration of the blood–brain barrier) and bimodal imaging capability, this novel and versatile multimodal nanoprobe could bring a new perspective for elucidating intracranial glioblastoma preoperative diagnosis and the accuracy of tumor resection.

scholarly journals PREDNISOLONE ENCAPSULATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES FOR TARGET DRUG DELIVERY – DESIGN AND QUANTIFICATION

2019 ◽  
pp. 126-131
Author(s):  
SUBASHINI RAJARAM ◽  
SENTHIL RAJAN DHARMALINGAM ◽  
SANTHOSE RANI A ◽  
SAPTHASRI R ◽  
VARSHA D ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Drug Release ◽  
Iron Oxide Nanoparticles ◽  
In Vitro Release ◽  
Physicochemical Characterization ◽  
Double Emulsion ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

Objective: The present study aimed to develop a novel type of superparamagnetic iron oxide nanoparticles (SPIONs) to deliver prednisolone at colon as a target site for the treatment of inflammatory bowel disease (IBD) such as ulcerative colitis and Crohn’s disease which may further progress to cancer. Methods: SPIONs were synthesized using a coprecipitation method. Further, it was encapsulated with prednisolone-polyethylene glycol by double emulsion method (W1/O/W2). The prepared formulations were characterized for its physicochemical characterization such as scanning electron microscopy, X-ray diffraction, particle size and zeta potential, encapsulation efficiency, and in vitro drug release. Results: The results reveal that the physicochemical property of the formulations complies with the standard values and in vitro release of prednisolone in the first 18 h, attains 57 and 58% and it reaches 71 and 75% at 24 h, and this is statistically significant (p˂0.0177). This release result implies that the drug release from the formulations is controllable and sustains manner. Conclusion: Our findings could be a promising approach for the delivery of prednisolone with enhanced half-life for the treatment of IBD through colon targeting.

scholarly journals Therapeutic Efficiency of Multiple Applications of Magnetic Hyperthermia Technique in Glioblastoma Using Aminosilane Coated Iron Oxide Nanoparticles: In Vitro and In Vivo Study

2020 ◽  
Vol 21 (3) ◽  
pp. 958 ◽  
Author(s):  
Gabriel Rego ◽  
Mariana Nucci ◽  
Javier Mamani ◽  
Fernando Oliveira ◽  
Luciana Marti ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Hyperthermia ◽  
Emission Tomography ◽  
Oxide Nanoparticles ◽  
Therapeutic Effects ◽  
Positron Emission

Magnetic hyperthermia (MHT) has been shown as a promising alternative therapy for glioblastoma (GBM) treatment. This study consists of three parts: The first part evaluates the heating potential of aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONa). The second and third parts comprise the evaluation of MHT multiple applications in GBM model, either in vitro or in vivo. The obtained heating curves of SPIONa (100 nm, +20 mV) and their specific absorption rates (SAR) stablished the best therapeutic conditions for frequencies (309 kHz and 557 kHz) and magnetic field (300 Gauss), which were stablished based on three in vitro MHT application in C6 GBM cell line. The bioluminescence (BLI) signal decayed in all applications and parameters tested and 309 kHz with 300 Gauss have shown to provide the best therapeutic effect. These parameters were also established for three MHT applications in vivo, in which the decay of BLI signal correlates with reduced tumor and also with decreased tumor glucose uptake assessed by positron emission tomography (PET) images. The behavior assessment showed a slight improvement after each MHT therapy, but after three applications the motor function displayed a relevant and progressive improvement until the latest evaluation. Thus, MHT multiple applications allowed an almost total regression of the GBM tumor in vivo. However, futher evaluations after the therapy acute phase are necessary to follow the evolution or tumor total regression. BLI, positron emission tomography (PET), and spontaneous locomotion evaluation techniques were effective in longitudinally monitoring the therapeutic effects of the MHT technique.

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

Shaping and Cellular Uptake of Folic Acid Coated Gold and Magnetite Nanoparticles

2019 ◽  
Vol 9 (2) ◽  
pp. 166-172
Author(s):  
Ahmed A.G. El-Shahawy ◽  
Gamal Elghnam ◽  
Alsayed A.M. Alsherbini
Keyword(s):  
Folic Acid ◽  
Iron Oxide ◽  
Tumor Cells ◽  
Iron Oxide Nanoparticles ◽  
Individual Cell ◽  
Oxide Nanoparticles ◽  
Cellular Compartment ◽  
Specific Tumor ◽  
Uv Visible

Background:Gold and Iron Oxide nanoparticles NPs play as nanocarriers for a specific drug delivery and contrast agents. Intercellular uptake of these nanoparticles and targeting to individual cell and sub-cellular compartment is essential.Objective:The aim of the current study is to evaluate the intracellular uptake of these NPs to specific tumor cells in vitro conjugated with folic acid with a goal of enhancing the efficiency of specific targeting to tumor cells.Methods:We synthesized the nanoparticles by a chemical method and characterized by UV-Visible, FTIR, XRD, and TEM.Results & Conclusion:The results revealed the conjugation of Gold and Iron Oxide nanoparticles with folic acid increased the intercellular uptake with high percent compared to non- conjugated nanoparticles.

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