Evaluation of Cleavable (Tyr3)-octreotate Derivatives for Longer Intracellular Probe Residence

2004 ◽  
Vol 15 (3) ◽  
pp. 647-657 ◽  
Author(s):  
Paul A. Whetstone ◽  
Hiromichi Akizawa ◽  
Claude F. Meares
Keyword(s):  
Intracellular Probe

scholarly journals A Review on Electroporation-Based Intracellular Delivery

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 3044 ◽  
Author(s):  
Junfeng Shi ◽  
Yifan Ma ◽  
Jing Zhu ◽  
Yuanxin Chen ◽  
Yating Sun ◽  
...  
Keyword(s):  
Viral Vectors ◽  
Safety Issue ◽  
Intracellular Delivery ◽  
Cellular Reprogramming ◽  
Gene Therapies ◽  
Potential Safety ◽  
Potential Applications ◽  
Dose Control ◽  
Intracellular Probe ◽  
New Generation

Intracellular delivery is a critical step in biological discoveries and has been widely utilized in biomedical research. A variety of molecular tools have been developed for cell-based gene therapies, including FDA approved CAR-T immunotherapy, iPSC, cell reprogramming and gene editing. Despite the inspiring results of these applications, intracellular delivery of foreign molecules including nucleic acids and proteins remains challenging. Efficient yet non-invasive delivery of biomolecules in a high-throughput manner has thus long fascinates the scientific community. As one of the most popular non-viral technologies for cell transfection, electroporation has gone through enormous development with the assist of nanotechnology and microfabrication. Emergence of miniatured electroporation system brought up many merits over the weakness of traditional electroporation system, including precise dose control and high cell viability. These new generation of electroporation systems are of considerable importance to expand the biological applications of intracellular delivery, bypassing the potential safety issue of viral vectors. In this review, we will go over the recent progresses in the electroporation-based intracellular delivery and several potential applications of cutting-edge research on the miniatured electroporation, including gene therapy, cellular reprogramming and intracellular probe.

scholarly journals Dual Fluorescence through Kasha’s Rule Breaking: An Unconventional Photomechanism for Intracellular Probe Design

2015 ◽  
Vol 119 (20) ◽  
pp. 6144-6154 ◽  
Author(s):  
Giuseppe Brancato ◽  
Giovanni Signore ◽  
Paolo Neyroz ◽  
Dario Polli ◽  
Giulio Cerullo ◽  
...  
Keyword(s):  
Probe Design ◽  
Dual Fluorescence ◽  
Rule Breaking ◽  
Intracellular Probe

Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode

2020 ◽  
Vol 173 ◽  
pp. 107866
Author(s):  
Klaus Koren ◽  
Nina Katharina Gravesen Salinas ◽  
Marco Santella ◽  
Maria Moßhammer ◽  
Marie-Caroline Müller ◽  
...  
Keyword(s):  
Intracellular Probe

scholarly journals Thyrotropin-releasing hormone increases cytosolic free Ca2+ in clonal pituitary cells (GH3 cells): direct evidence for the mobilization of cellular calcium.

1984 ◽  
Vol 99 (1) ◽  
pp. 83-87 ◽  
Author(s):  
W Schlegel ◽  
C B Wollheim
Keyword(s):  
Receptor Activation ◽  
Thyrotropin Releasing Hormone ◽  
Cell Surface Receptor ◽  
Gh3 Cells ◽  
Pituitary Cells ◽  
Releasing Hormone ◽  
Surface Receptor ◽  
Intracellular Probe ◽  
Rat Pituitary Cells ◽  
Free Media

Changes in the cytosolic free Ca2+ concentration following cell surface receptor activation have been proposed to mediate a wide variety of cellular responses. Using the specific Ca2+ chelator quin2 as a fluorescent intracellular probe, we measured the Ca2+ levels in the cytosol of clonal rat pituitary cells, GH3 cells. We demonstrate that thyrotropin-releasing hormone (TRH) at nanomolar concentrations leads to a rapid and transient increase in cytosolic Ca2+. This increase was found to occur in Ca2+-free media in the presence of EGTA, thus at extracellular Ca2+ levels that are below the cytosolic concentrations, and was not prevented by verapamil, a Ca2+ channel blocker. Depolarization of GH3 cells with K+, which can mimic the action of TRH on prolactin release, increased cytosolic Ca2+ levels only in the presence of free extracellular Ca2+, and this increase could be blocked by verapamil. These data show that the mobilization of intracellular Ca2+ due to TRH action that has been proposed by previous studies actually leads to an increase in cytosolic free Ca2+. The kinetic features of this response emphasize the key role of cytosolic free Ca2+ in stimulus-secretion coupling.

Nanowire lasers as intracellular probes

Nanoscale ◽  
2018 ◽  
Vol 10 (20) ◽  
pp. 9729-9735 ◽  
Author(s):  
Xiaoqin Wu ◽  
Qiushu Chen ◽  
Peizhen Xu ◽  
Yu-Cheng Chen ◽  
Biming Wu ◽  
...  
Keyword(s):  
Cadmium Sulfide ◽  
Single Cell ◽  
Cds Nanowire ◽  
Intracellular Probe ◽  
Nanowire Lasers

We investigate a cadmium sulfide (CdS) nanowire (NW) laser that is spontaneously internalized into a single cell to serve as a stand-alone intracellular probe.

EGF modulation of Na+/H+ antiport in rat hepatocytes: different sensitivity in adult and fetal cells

1996 ◽  
Vol 270 (3) ◽  
pp. C841-C847 ◽  
Author(s):  
S. Incerpi ◽  
S. Spagnuolo ◽  
F. Terenzi ◽  
S. Leoni
Keyword(s):  
Kinase Activity ◽  
Egf Receptor ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Fetal Hepatocytes ◽  
Kinase C ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth ◽  
Intracellular Probe ◽  
High Concentrations

The modulation by epidermal growth factor (EGF) of the Na+/H+ antiport in fetal and adult rat hepatocytes was studied in nominally HCO3- free solution. EGF (10 nM) activated the antiport in adult rat hepatocytes by 0.22 +/- 0.03 (mean +/- SD;n=10) pH units over basal value, measured with the fluorescent pH-sensitive intracellular probe, 2',7'-bis(carboxyethyl)-5(6)- carboxyfluorescein (BCECF). The effect of EGF was inhibited by amiloride analogue 5-(N-ethyl-N-isopropyl) amiloride (EIPA), by ouabain, inhibitor of the Na+ pump, and by erbstatin analogue, an inhibitor of the tyrosine kinase activity of the EGF receptor. The effect of EGF on Na+/H+ antiport in adult rat hepatocytes appeared to be mediated by both protein kinase C (PKC) and G protein system. No effect of EGF and phorbol 12-myristate 13-acetate, an activator of PKC, on the Na+/H+ antiport was observed in fetal hepatocytes of 20 and 22 days. A different sensitivity of the antiport to high concentrations of amiloride and EIPA suggests that altered amount of the Na+/H+ antiport units or different isoforms could be expressed in fetal compared with adult cells.

Sign in / Sign up

Export Citation Format

Share Document