A Tc-99m-Labeled Long Chain Fatty Acid Derivative for Myocardial Imaging

2004 ◽  
Vol 15 (2) ◽  
pp. 389-393 ◽  
Author(s):  
Yasuhiro Magata ◽  
Takayoshi Kawaguchi ◽  
Misa Ukon ◽  
Norio Yamamura ◽  
Tomoya Uehara ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Derivative ◽  
Fatty Acid Derivative ◽  
Chain Fatty Acid ◽  
Myocardial Imaging ◽  
Long Chain Fatty Acid ◽  
Long Chain

Confocal analysis of hepatocellular long-chain fatty acid uptake

1995 ◽  
Vol 269 (6) ◽  
pp. G842-G851 ◽  
Author(s):  
C. Elsing ◽  
U. Winn-Borner ◽  
W. Stremmel
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acid Derivative ◽  
Fatty Acid Derivative ◽  
Fatty Acid Uptake ◽  
Chain Fatty Acid ◽  
Acid Uptake ◽  
Long Chain Fatty Acid ◽  
Membrane Interaction ◽  
Long Chain

Transmembrane transport and cytosolic accumulation of fatty acids were investigated using confocal laser scanning microscopy (cLSM). A Zeiss LSM 310 system was used to determine the uptake of the fluorescent fatty acid derivative 12-(N-methyl)-N-[(7-nitrobenz-2-oxa-1,3- diazol-4-yl)amino]octadecanoic acid (12-NBD stearate) (C18) in single rat hepatocytes. Uptake was a saturable process with a Michaelis-Menten constant value of 68 nM. Initial uptake velocity was dependent on extracellular presence of albumin and beta-lactoglobulin. Absence of albumin reduced uptake to 32 +/- 16% (P < 0.01) of control values. In the presence of unlabeled stearate, uptake of 12-NBD stearate was lowered to 49 +/- 12% (P < 0.01). Ion substitution experiments showed no sodium dependency of uptake. Increase in membrane potential led to a pronounced accumulation of the fatty acid derivative within the plasma membrane and in the adjacent cytoplasmic compartment, whereas membrane depolarization had no effect on uptake rates. In separate experiments line scans through representative hepatocytes were analyzed to generate "x-t" plots. 12-NBD stearate showed a fluorescence pattern with prominent staining of the area of the plasma membrane and the adjacent cytoplasm, dependent on the presence of extracellular albumin. For the hepatocellular cytosolic accumulation process of 12-NBD stearate a diffusion constant of 22.2 +/- 6.2 x 10(-9) cm2/s was calculated. In contrast to the long-chain fatty acid derivative 12-NBD stearate, short (C5)- and medium (C11)-chain fatty acids revealed no membrane interaction with hepatocytes. Erythrocytes also lacked a membrane interaction process for 12-NBD stearate. In conclusion, it was demonstrated that cLSM is capable of directly evaluating the cellular fatty acid uptake process at a subcellular level.

Prolongation of the Intravascular Retention of Hemoglobin Modified with a Long-Chain Fatty Acid Derivative

1994 ◽  
Vol 22 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Scott A. Fowler ◽  
Mark Andracki ◽  
Gerald Hurst ◽  
Vidya A. Honkan ◽  
Joseph Waldorf ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Derivative ◽  
Fatty Acid Derivative ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Long Chain

scholarly journals Long-chain fatty acid-induced changes in gene expression in neonatal cardiac myocytes

2000 ◽  
Vol 41 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Karin A. J.M. van der Lee ◽  
Michaël M. Vork ◽  
Johan E. De Vries ◽  
Peter H.M. Willemsen ◽  
Jan F.C. Glatz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Cardiac Myocytes ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Induced Changes ◽  
Long Chain
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