Synthesis of Trifunctional PNA−Benzophenone Derivatives for Mitochondrial Targeting, Selective DNA Binding, and Photo-Cross-Linking

2003 ◽  
Vol 14 (5) ◽  
pp. 962-966 ◽  
Author(s):  
Günther F. Ross ◽  
Paul M. Smith ◽  
Alistair McGregor ◽  
Douglass M. Turnbull ◽  
Robert N. Lightowlers
Keyword(s):  
Dna Binding ◽  
Cross Linking ◽  
Mitochondrial Targeting ◽  
Selective Dna Binding

Biochemical Chgaracterization of Recombinant Baculovirus 373 K/E p53 Protein

2018 ◽  
Vol 9 (03) ◽  
pp. 20204-20223
Author(s):  
Maghsoudi, Hossein ◽  
U Pati
Keyword(s):  
Dna Binding ◽  
P53 Protein ◽  
Expression System ◽  
Gel Filtration ◽  
Recombinant Baculovirus ◽  
Baculovirus Expression System ◽  
Cross Linking ◽  
Mobility Shift ◽  
Dna Complexes ◽  
P53 Antibodies

In this study, we expressed and purified the recombinant baculovirus 373 K/E p53 protein in a baculovirus expression system to characterize this mutant and compare it with wild type p53. Gel- filtration chromatography and chemical cross-linking experiments indicated that purified recombinant baculovirus 373 K/E p53 protein assembles into multimeric forms ranging from tetramers to polymers. Gel-mobility shift assays and protein-DNA cross-linking studies demonstrated that the recombinant protein binds, to a consensus DNA target as a dimer but that additional p53 mutant molecules may then associate with the preformed p53-dimer-DNA complexes to form a larger p53_DNA complexes. These observations suggest that the p53 mutant tetramers and polymers that forms the minimal p53 mutant complex in solution dissociated upon DNA binding to form p53 mutant dimmer DNA complexes. The DNA binding activity of this mutant was then investigated using electrophoretic mobility shift assays as well as supershift assay with anti-p53 antibodies. Binding of the anti-p53 antibody PAb421to the oligomerization promoting domain on p53 stimulated the sequential formation of both the p53_dimer DNA and larger p53-DNA complexes

scholarly journals The molecular basis of selective DNA binding by the BRG1 AT-hook and bromodomain

2020 ◽  
Vol 1863 (8) ◽  
pp. 194566
Author(s):  
Julio C. Sanchez ◽  
Liyang Zhang ◽  
Stefania Evoli ◽  
Nicholas J. Schnicker ◽  
Maria Nunez-Hernandez ◽  
...  
Keyword(s):  
Dna Binding ◽  
Molecular Basis ◽  
Selective Dna Binding

scholarly journals Site-Selective DNA Binding Drugs

2003 ◽  
Vol 10 (10) ◽  
pp. 895-897 ◽  
Author(s):  
Scot W. Ebbinghaus
Keyword(s):  
Dna Binding ◽  
Selective Dna Binding ◽  
Site Selective

scholarly journals Correction to Metal-Based Netropsin Mimics Showing AT-Selective DNA Binding and DNA Cleavage Activity at Red Light

2019 ◽  
Vol 58 (14) ◽  
pp. 9514-9514
Author(s):  
Ashis K. Patra ◽  
Tuhin Bhowmick ◽  
Suryanarayanarao Ramakumar ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Red Light ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Selective Dna Binding
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