Chemical modification of silk fibroin with cyanuric chloride-activated poly(ethylene glycol): Analyses of reaction site by proton NMR spectroscopy and conformation of the conjugates

1993 ◽  
Vol 4 (6) ◽  
pp. 554-559 ◽  
Author(s):  
Yohko Gotoh ◽  
Masuhiro Tsukada ◽  
Norihiko Minoura
Keyword(s):  
Nmr Spectroscopy ◽  
Ethylene Glycol ◽  
Chemical Modification ◽  
Silk Fibroin ◽  
Proton Nmr ◽  
Cyanuric Chloride ◽  
Reaction Site ◽  
Proton Nmr Spectroscopy ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Synthesis of Mono- and Dithiols of Tetraethylene Glycol and Poly(ethylene glycol)s via Enzyme Catalysis

Catalysts ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 228 ◽  
Author(s):  
Prajakatta Mulay ◽  
Gayatri Shrikhande ◽  
Judit Puskas
Keyword(s):  
Nmr Spectroscopy ◽  
Ethylene Glycol ◽  
1H Nmr ◽  
Enzyme Catalysis ◽  
1H Nmr Spectroscopy ◽  
13C Nmr Spectroscopy ◽  
Tetraethylene Glycol ◽  
Candida Antarctica Lipase ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

This paper investigates the transesterification of methyl 3-mercaptopropionate (MP-SH) with tetraethylene glycol (TEG) and poly(ethylene glycol)s (PEG)s catalyzed by Candida antarctica Lipase B (CALB) without the use of solvent (in bulk). The progress of the reactions was monitored by 1H-NMR spectroscopy. We found that the reactions proceeded in a step-wise manner, first producing monothiols. TEG-monothiol was obtained in 15 min, while conversion to dithiol took 8 h. Monothiols from PEGs with Mn = 1000 and 2050 g/mol were obtained in 8 and 16 h, respectively. MALDI-ToF mass spectrometry verified the absence of dithiols. The synthesis of dithiols required additional fresh CALB and MP-SH. The structure of the products was confirmed by 1H-NMR and 13C-NMR spectroscopy. Enzyme catalysis was found to be a powerful tool to effectively synthesize thiol-functionalized TEGs and PEGs.

scholarly journals Preparation of silk fibroin-poly(ethylene glycol) conjugate films through click chemistry

2011 ◽  
Vol 60 (12) ◽  
pp. 1737-1744 ◽  
Author(s):  
Sandra Sampaio ◽  
Teresa M. R. Miranda ◽  
Jorge G. Santos ◽  
Graça M. B. Soares
Keyword(s):  
Ethylene Glycol ◽  
Click Chemistry ◽  
Silk Fibroin ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Dual mode gelation behavior of silk fibroin microgel embedded poly(ethylene glycol) hydrogels

2016 ◽  
Vol 4 (26) ◽  
pp. 4574-4584 ◽  
Author(s):  
S. Ryu ◽  
H. H. Kim ◽  
Y. H. Park ◽  
C.-C. Lin ◽  
I. C. Um ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Cross Linking ◽  
Poly Ethylene Glycol ◽  
Dual Mode ◽  
Hydrogel Formation ◽  
Gelation Behavior ◽  
Poly Ethylene

Hydrogel formation by more than two cross-linking mechanisms is preferred for the sophisticated manipulation of hydrogel properties.

Cleavable poly(ethylene glycol) branched chain-modified Antheraea pernyi silk fibroin as a gene delivery carrier

Nanomedicine ◽  
2021 ◽  
Author(s):  
Xueping Liu ◽  
Hong Luo ◽  
Longxing Niu ◽  
Yanfei Feng ◽  
Peng Pan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Plasma Proteins ◽  
Kidney Cells ◽  
Poly Ethylene Glycol ◽  
Normal Cells ◽  
Antheraea Pernyi ◽  
Poly Ethylene ◽  
Branched Chain

Aim: To obtain a gene carrier that can effectively deliver loaded therapeutic genes to tumor cells, avoid toxic effects on normal cells and reduce nonspecific adsorption of plasma proteins. Methods: The conjugate of poly(ethylene glycol) (PEG) and MMP2SSP (PEG-MMP2SSP) was covalently coupled to cationized Antheraea pernyi silk fibroin (CASF) through disulfide bond exchange reaction to obtain a PEG-MMP2SSP-modified CASF (CASFMP). Results: The PEG chains were effectively cleaved from the CASFMP by MMP2. CASFMP/pDNA complexes inhibited human fibrosarcoma cell proliferation, and its cytotoxicity to human normal embryonic kidney cells was significantly lower than that of poly(ethylenimine)/pDNA after coculturing with cells for 24 h. Conclusion: CASFMP is a promising compound for use in gene therapy.

Semi-interpenetrating polymer networks composed of silk fibroin and poly(ethylene glycol) for wound dressing

2008 ◽  
Vol 3 (3) ◽  
pp. 034115 ◽  
Author(s):  
HaeYong Kweon ◽  
Joo-hong Yeo ◽  
Kwang-gill Lee ◽  
Hyun Chul Lee ◽  
Hee Sam Na ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Wound Dressing ◽  
Polymer Networks ◽  
Interpenetrating Polymer Networks ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Development of silk fibroin modified poly(l-lactide)–poly(ethylene glycol)–poly(l-lactide) nanoparticles in supercritical CO2

2014 ◽  
Vol 268 ◽  
pp. 118-125 ◽  
Author(s):  
Zheng Zhao ◽  
Yi Li ◽  
Yu Zhang ◽  
Ai-Zheng Chen ◽  
Gang Li ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Supercritical Co2 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Surface modification of silk fibroin with poly(ethylene glycol) for antiadhesion and antithrombotic applications

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Charu Vepari ◽  
Douglas Matheson ◽  
Larry Drummy ◽  
Rajesh Naik ◽  
David L. Kaplan
Keyword(s):  
Surface Modification ◽  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Physical properties and structure of poly(ethylene glycol)-silk fibroin conjugate films

Polymer ◽  
1997 ◽  
Vol 38 (2) ◽  
pp. 487-490 ◽  
Author(s):  
Yohko Gotoh ◽  
Masuhiro Tsukada ◽  
Teruhiko Baba ◽  
Norihiko Minoura
Keyword(s):  
Ethylene Glycol ◽  
Physical Properties ◽  
Silk Fibroin ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene
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