Peroxidase-linked anti-basic fibroblast growth factor monoclonal antibody Fab' conjugates: Application for two-site enzyme immunoassay and immunohistochemical detection

1993 ◽  
Vol 4 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Masayuki Kurobe ◽  
Yoshifumi Takei ◽  
Takashi Fukatsu ◽  
Akemi Kato ◽  
Kyozo Hayashi
Keyword(s):  
Monoclonal Antibody ◽  
Growth Factor ◽  
Enzyme Immunoassay ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Immunohistochemical Detection

scholarly journals In Situ Assay of Basic Fibroblast Growth Factor in Cultured Cells with a Specific Monoclonal Antibody.

1993 ◽  
Vol 18 (5) ◽  
pp. 333-343
Author(s):  
Masami Minemura ◽  
Yoshino Yoshitake ◽  
Kouichi Matsuzaki ◽  
Akiharu Watanabe ◽  
Katsuzo Nishikawa
Keyword(s):  
Monoclonal Antibody ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cultured Cells ◽  
Fibroblast Growth ◽  
Specific Monoclonal Antibody ◽  
In Situ Assay

Fluorometric Enzyme Immunoassay of Basic Fibroblast Growth Factor with Monoclonal Antibodies

1992 ◽  
Vol 38 (10) ◽  
pp. 2121-2123 ◽  
Author(s):  
M Kurobe ◽  
A Kato ◽  
Y Takei ◽  
K Hayashi
Keyword(s):  
Detection Limit ◽  
Growth Factor ◽  
Enzyme Immunoassay ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Preliminary Measurement ◽  
Assay Tube ◽  
Secondary Antibodies ◽  
Apparently Healthy

Abstract We compared three different strategies for measuring basic fibroblast growth factor (bFGF) by fluorometric enzyme immunoassay (EIA). After optimizing conditions, we found that a primary anti-bFGF MAb directly conjugated with peroxidase gave the best detection limit for recombinant bFGF (approximately 30 ng/L, 3 pg/assay tube) in a two-site sandwich assay. The detection limit of methods based on biotinylated primary MAbs or on secondary antibodies followed by streptavidin-conjugated peroxidase was slightly lower than that of the above method. Using the most sensitive EIA examined in this study, we made a preliminary measurement of immunoreactive bFGF in sera of apparently healthy people and found it to be 190 (SD 32) ng/L (n = 48), in agreement with an earlier reported value (30-206 ng/L). Also, the concentration of immunoreactive bFGF in sera was above normal in 19 of 31 patients with stomach cancer.

A sensitive enzyme immunoassay for human basic fibroblast growth factor

1991 ◽  
Vol 175 (1) ◽  
pp. 229-235 ◽  
Author(s):  
Hiroyuki Watanabe ◽  
Akira Hori ◽  
Masaharu Seno ◽  
Yoshio Kozai ◽  
Koichi Igarashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Enzyme Immunoassay ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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