scholarly journals Synthesis, Spectral Properties, and Detection Limits of Reactive Squaraine Dyes, a New Class of Diode Laser Compatible Fluorescent Protein Labels

2000 ◽  
Vol 11 (3) ◽  
pp. 438-438 ◽  
Author(s):  
Bernhard Oswald ◽  
Leonid Patsenker ◽  
Josef Duschl ◽  
Henryk Szmacinski ◽  
Otto S. Wolfbeis ◽  
...  
Keyword(s):  
Diode Laser ◽  
Spectral Properties ◽  
Fluorescent Protein ◽  
Detection Limits ◽  
Squaraine Dyes ◽  
New Class

Synthesis, Spectral Properties, and Detection Limits of Reactive Squaraine Dyes, a New Class of Diode Laser Compatible Fluorescent Protein Labels

1999 ◽  
Vol 10 (6) ◽  
pp. 925-931 ◽  
Author(s):  
Bernhard Oswald ◽  
Leonid Patsenker ◽  
Josef Duschl ◽  
Henryk Szmacinski ◽  
Otto S. Wolfbeis ◽  
...  
Keyword(s):  
Diode Laser ◽  
Spectral Properties ◽  
Fluorescent Protein ◽  
Detection Limits ◽  
Squaraine Dyes ◽  
New Class

Fluorescent protein expression in temperature tolerant and susceptible reef-building corals

2021 ◽  
pp. 1-10
Author(s):  
Exequiel Gabriel S. Dizon ◽  
Jeric P. Da-Anoy ◽  
Melissa S. Roth ◽  
Cecilia Conaco
Keyword(s):  
Spectral Properties ◽  
Coral Species ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Antioxidant Properties ◽  
Variable Expression ◽  
Acropora Digitifera ◽  
Insight Into

Abstract Fluorescent proteins (FPs) are reported to play an important role as photoprotectants and antioxidants in corals subjected to stressful conditions. Identifying the various FP genes expressed and FP gene expression patterns under stress in diverse coral species can provide insight into FP function. In this study, we identified 16 putative FP homologues from the transcriptomes of corals with varying susceptibility to elevated temperature, including Acropora digitifera, Favites colemani, Montipora digitata and Seriatopora caliendrum. Each coral expressed a different complement of FP transcripts, which were predicted to have distinct spectral properties. The most diverse and abundant repertoire of FP transcripts, including at least 6 green FPs, were expressed in the temperature-tolerant coral, F. colemani. In comparison, the other corals expressed fewer FP types. Specific FP transcripts exhibited variable expression profiles in coral fragments subjected to 32 ± 1 °C (treatment) or 28 ± 1 °C (control) for up to 72 h, suggesting that distinct FPs may have different roles. Further studies on the expression of the proteins encoded by these FP transcripts, their fluorescence activity, tissue localization, and possible antioxidant properties, are needed to reveal their contribution to thermal stress tolerance in certain species of corals.

Exploring Structural Dynamics and Optical Properties of UnaG Fluorescent Protein upon N57 Mutations

2021 ◽  
Author(s):  
Mohammad Asad ◽  
Adèle D Laurent
Keyword(s):  
Optical Properties ◽  
Structural Dynamics ◽  
Fluorescent Protein ◽  
Green Light ◽  
Endogenous Ligand ◽  
New Class ◽  
Fluorescence Protein

UnaG is a new class of fluorescence protein for which an endogenous ligand namely bilirubin (BLR) is playing the role of the chromophore. Upon photoexcitation, holoUnaG emits the green light....

scholarly journals Excited-state locked amino analogues of the green fluorescent protein chromophore with a giant Stokes shift

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38730-38734 ◽  
Author(s):  
Snizhana O. Zaitseva ◽  
Dilara A. Farkhutdinova ◽  
Nadezhda S. Baleeva ◽  
Alexander Yu. Smirnov ◽  
Marina B. Zagudaylova ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Stokes Shift ◽  
Large Stokes Shift ◽  
New Class ◽  
Green Fluorescent

We design a new class of excited-state locked GFP chromophores which intrinsically exhibit a very large Stokes shift.

scholarly journals Switchable fluorescence by click reaction of a novel azidocarbazole dye

RSC Advances ◽  
2014 ◽  
Vol 4 (23) ◽  
pp. 11528-11534 ◽  
Author(s):  
Anna Hörner ◽  
Daniel Volz ◽  
Tobias Hagendorn ◽  
Daniel Fürniss ◽  
Lutz Greb ◽  
...  
Keyword(s):  
Smart Materials ◽  
Spectral Properties ◽  
Click Reaction ◽  
New Class

Smart materials: A demand for switchable tags led us to the design of a new class of pre-fluorophores. We achieved this by using a non-fluorescent N-(4-azidophenyl)-carbazole tag which turns fluorescent by click reaction with alkynes and cyclooctynes. Syntheses and spectral properties were investigated.

Syntheses and spectral properties of tropocoronands, a new class of versatile metal-complexing macrocycles derived from aminotropone imines

1986 ◽  
Vol 25 (19) ◽  
pp. 3400-3407 ◽  
Author(s):  
Arie Zask ◽  
Nina Gonnella ◽  
Koji Nakanishi ◽  
Christopher J. Turner ◽  
Seichi Imajo ◽  
...  
Keyword(s):  
Spectral Properties ◽  
New Class

scholarly journals Orange Fluorescent Proteins: Filling the Spectral Gap

2014 ◽  
Vol 70 (a1) ◽  
pp. C1670-C1670
Author(s):  
Sergei Pletnev ◽  
Daria Shcherbakova ◽  
Oksana Subach ◽  
Vladimir Malashkevich ◽  
Steven Almo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Spectral Properties ◽  
Molecular Mechanisms ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Stokes Shift ◽  
Emission Wavelength ◽  
Microscopy Imaging ◽  
Large Stokes Shift

Fluorescent proteins (FPs) have become valuable tools for molecular biology, biochemistry, medicine, and cancer research. Starting from parent green fluorescent protein (GFP), most challenging task of the FPs studies was the development of FPs with longer excitation/emission wavelength. This pursuit was motivated by advantages of so-called red-shifted FPs, namely, lower background of cellular autofluorescence in microscopy, lower light scattering and reduced tissue absorbance of longer wavelengths for in vivo imaging. In addition to FPs with regular spectral properties, there are proteins of other types available, including FPs with a large Stokes shift and photoconvertible FPs. These special kinds of FPs have become useful in super-resolution microscopy, imaging of enzyme activities, protein-protein interactions, photolabeling, and in vivo imaging. According to their emission wavelength, red-shifted FPs could be divided in the following groups: 520-540 nm yellow FPs (YFPs), 540-570 nm orange FPs (OFPs), 570-620 nm red FPs (RFPs), and > 620 nm far-RFPs. Red shift of the excitation/emission bands of these FPs is predominantly achieved by extension of the conjugated system of the chromophore and its protonation/deprotonation. The variety of spectral properties of FPs (excitation and emission wavelength, quantum yield, brightness, photo- and pH- stability, photoconversion, large Stokes shift, etc) results from the different chromophore structures and its interactions with surrounding amino acid residues. In this work we focus on structural studies and molecular mechanisms of FPs with orange emission.

Presynaptic Calcium Measurements at Physiological Temperatures Using a New Class of Dextran-Conjugated Indicators

2004 ◽  
Vol 92 (1) ◽  
pp. 591-599 ◽  
Author(s):  
Michael Beierlein ◽  
Kyle R. Gee ◽  
Vladimir V. Martin ◽  
Wade G. Regehr
Keyword(s):  
Neurotransmitter Release ◽  
Fluorescent Protein ◽  
Temperature Dependent ◽  
Parallel Fibers ◽  
New Class ◽  
Calcium Indicators ◽  
Presynaptic Calcium ◽  
Green Fluorescent ◽  
Peak Emission

Presynaptic calcium (Capre) has been studied extensively because of its role in triggering and modulating neurotransmitter release. Although calcium regulation and calcium-driven processes can be strongly temperature dependent, technical difficulties have limited most studies of Capre to temperatures well below the physiological range. Here we assessed the use of membrane-permeant acetoxymethyl (AM) indicators and dextran-conjugated indicators for measuring Capre at physiological temperatures. A comparison of these two types of indicators loaded into parallel fibers of rat cerebellar slices revealed striking differences. AM indicators were rapidly extruded from axons and presynaptic terminals and therefore cannot be used for long-term measurements at high temperatures. In contrast, dextran-conjugated indicators were retained within parallel fibers and are therefore well suited to measuring Capre at physiological temperatures. The limited number of dextran indicators available prompted us to synthesize three new indicators that show peak emission in the red (575–600 nm). These indicators allow for simultaneous use of multiple calcium indicators that can be readily distinguished on the basis of excitation and emission wavelengths, use of excitation and emission wavelengths that are relatively insensitive to tissue autofluorescence, and measurements in systems with expression of green fluorescent protein (GFP). Thus we find that dextran-conjugated indicators are well suited to long-term recordings of Capre at physiological temperatures and that the development of new red indicators greatly extends their utility.

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