Long-Range Electron Transfer Between Proline-Bridged Aromatic Amino Acids

1998 ◽  
pp. 131-143 ◽  
Author(s):  
K. Bobrowski ◽  
J. Poznański ◽  
J. Holcman ◽  
K. L. Wierzchowski
Keyword(s):  
Amino Acids ◽  
Electron Transfer ◽  
Long Range ◽  
Aromatic Amino Acids

scholarly journals Theoretical Analyses of Photoinduced Electron Transfer from Aromatic Amino Acids to the Excited Flavins in Some Flavoproteins

10.5772/38078 ◽  
2012 ◽  
Author(s):  
Kiattisak Lugsanangarm ◽  
Nadtanet Nunthaboot ◽  
Somsak Pianwanit ◽  
Sirirat Kokpol ◽  
Fumio Tanak
Keyword(s):  
Amino Acids ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Aromatic Amino Acids ◽  
Theoretical Analyses

scholarly journals Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens

mBio ◽  
2013 ◽  
Vol 4 (2) ◽  
Author(s):  
Madeline Vargas ◽  
Nikhil S. Malvankar ◽  
Pier-Luc Tremblay ◽  
Ching Leang ◽  
Jessica A. Smith ◽  
...  
Keyword(s):  
Amino Acids ◽  
Electron Transport ◽  
Long Range ◽  
Microbial Fuel Cells ◽  
Aromatic Amino Acids ◽  
Geobacter Sulfurreducens ◽  
Control Strain ◽  
Content Type ◽  
Current Production ◽  
Extracellular Electron Transport

ABSTRACTIt has been proposed thatGeobacter sulfurreducensrequires conductive pili for long-range electron transport to Fe(III) oxides and for high-density current production in microbial fuel cells. In order to investigate this further, we constructed a strain ofG. sulfurreducens, designated Aro-5, which produced pili with diminished conductivity. This was accomplished by modifying the amino acid sequence of PilA, the structural pilin protein. An alanine was substituted for each of the five aromatic amino acids in the carboxyl terminus of PilA, the region in whichG. sulfurreducensPilA differs most significantly from the PilAs of microorganisms incapable of long-range extracellular electron transport. Strain Aro-5 produced pili that were properly decorated with the multihemec-type cytochrome OmcS, which is essential for Fe(III) oxide reduction. However, pili preparations of the Aro-5 strain had greatly diminished conductivity and Aro-5 cultures were severely limited in their capacity to reduce Fe(III) compared to the control strain. Current production of the Aro-5 strain, with a graphite anode serving as the electron acceptor, was less than 10% of that of the control strain. The conductivity of the Aro-5 biofilms was 10-fold lower than the control strain’s. These results demonstrate that the pili ofG. sulfurreducensmust be conductive in order for the cells to be effective in extracellular long-range electron transport.IMPORTANCEExtracellular electron transfer byGeobacterspecies plays an important role in the biogeochemistry of soils and sediments and has a number of bioenergy applications. For example, microbial reduction of Fe(III) oxide is one of the most geochemically significant processes in anaerobic soils, aquatic sediments, and aquifers, andGeobacterorganisms are often abundant in such environments.Geobacter sulfurreducensproduces the highest current densities of any known pure culture, and close relatives are often the most abundant organisms colonizing anodes in microbial fuel cells that harvest electricity from wastewater or aquatic sediments. The finding that a strain ofG. sulfurreducensthat produces pili with low conductivity is limited in these extracellular electron transport functions provides further insight into these environmentally significant processes.

INTRAMOLECULAR EXCITED ENERGY TRANSFER PATHWAYS IN PROTEINS

2008 ◽  
Vol 07 (01) ◽  
pp. 91-102
Author(s):  
LEONARDO R. LAREO ◽  
JANNETH GONZÁLEZ
Keyword(s):  
Amino Acids ◽  
Electron Transfer ◽  
Energy Transfer ◽  
Protein Structure ◽  
Aromatic Amino Acids ◽  
Theoretical Background ◽  
Biological Processes ◽  
Computational Approaches ◽  
Orbital Interactions ◽  
Conducting Materials

The transfer of energy perturbations within protein structure is an important phenomenon in many biological processes. In particular, the transfer of energy perturbations within a molecule in the absence of electron transfer is critical to the understanding of such processes as signaling involving receptors, channels, and enzymes among others, and to the design and development of relevant conducting materials. In this work, we have proposed a mechanism to explain this nonradiative, nonelectron energy transfer based on the π-orbital interactions of aromatic amino acids. Additionally, some theoretical background and possible computational approaches are presented as support for the proposal.

scholarly journals Extended Electron-Transfer in Animal Cryptochromes Mediated by a Tetrad of Aromatic Amino Acids

2016 ◽  
Vol 111 (2) ◽  
pp. 301-311 ◽  
Author(s):  
Daniel Nohr ◽  
Sophie Franz ◽  
Ryan Rodriguez ◽  
Bernd Paulus ◽  
Lars-Oliver Essen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Electron Transfer ◽  
Aromatic Amino Acids

Classification of the mechanisms of photoinduced electron transfer from aromatic amino acids to the excited flavins in flavoproteins

2015 ◽  
Vol 17 (26) ◽  
pp. 16813-16825 ◽  
Author(s):  
Fumio Tanaka ◽  
Kiattisak Lugsanangarm ◽  
Nadtanet Nunthaboot ◽  
Arthit Nueangaudom ◽  
Somsak Pianwanit ◽  
...  
Keyword(s):  
Amino Acids ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Aromatic Amino Acids ◽  
Wavelength Dependence ◽  
Emission Wavelength ◽  
Donor Acceptor ◽  
The Relationship

Emission wavelength-dependence of the relationship between logarithmic ET rate vs. donor–acceptor distance in pyranose 2-oxidase.

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