Au Nanoparticles–3D Graphene Hydrogel Nanocomposite To Boost Synergistically in Situ Detection Sensitivity toward Cell-Released Nitric Oxide

2015 ◽  
Vol 7 (4) ◽  
pp. 2726-2734 ◽  
Author(s):  
Jialin Li ◽  
Jiale Xie ◽  
Lixia Gao ◽  
Chang Ming Li
Keyword(s):  
Nitric Oxide ◽  
Au Nanoparticles ◽  
Detection Sensitivity ◽  
3D Graphene ◽  
In Situ Detection ◽  
Hydrogel Nanocomposite

Facile one-pot synthesis of Au nanoparticles decorated porous α-Fe2O3nanorods for in situ detection of VOCs

RSC Advances ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 369-373 ◽  
Author(s):  
Shurong Wang ◽  
Hongxin Zhang ◽  
Yanshuang Wang ◽  
Liwei Wang ◽  
Zhe Gong
Keyword(s):  
Au Nanoparticles ◽  
One Pot ◽  
One Pot Synthesis ◽  
In Situ Detection

scholarly journals Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in Saliva

2021 ◽  
Author(s):  
Luke Lee ◽  
Fei Liu ◽  
Yuchao Chen ◽  
Gianluca Roma
Keyword(s):  
Detection Sensitivity ◽  
Testing Methods ◽  
Health Crisis ◽  
Viral Antigens ◽  
Primary Care Settings ◽  
Sample Pooling ◽  
Chemiluminescent Immunoassay ◽  
Pooling Strategy ◽  
In Situ Detection

Abstract The COVID-19 pandemic has become an immense global health crisis. However, the lack of efficient and sensitive on-site testing methods limits early detection for timely isolation and intervention. Here, we present a Quantitative and Ultrasensitive in-situ Immunoassay Technology for SARS-CoV-2 detection in saliva (QUIT SARS-CoV-2). Our nanoporous membrane resonator generates a rapid oscillating flow to purify and concentrate SARS-CoV-2 virus in saliva by 40 folds for in-situ detection of viral antigens based on chemiluminescent immunoassay within 20 min. This method achieved a detection sensitivity below 10 0 copies/mL viral load, comparable to the bench-top PCR equipment. The portable QUIT SARS-CoV-2 system, allowing rapid and accurate on-site viral screen with high-throughput sample pooling strategy, can be performed at the primary care settings and substantially improve the detection and prevention of COVID-19.

scholarly journals Direct in situ reverse transcriptase-polymerase chain reaction

2001 ◽  
Vol 281 (2) ◽  
pp. C726-C732 ◽  
Author(s):  
Rajesh Kher ◽  
Robert Bacallao
Keyword(s):  
Reverse Transcriptase ◽  
Restriction Enzymes ◽  
Cellular Level ◽  
Detection Sensitivity ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
A Cell ◽  
Polymerase Chain ◽  
In Situ Detection

In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell. However, the available methods are fraught with problems of nonspecific amplifications as a result of mispriming and/or amplification from partially digested residual genomic DNA in tissue. Herein, we demonstrate that nonspecific background amplification can be eliminated by pretreatment of samples with restriction enzymes before DNase I digestion. Primers tagged with a far-red shifted fluorescent dye such as Cy5 in PCR reactions allow identification of target mRNA by fluorescence microscopy. These novel modifications lead to increased specificity and rapid in situ detection of cellular mRNA and thus may be used for pathological diagnosis.

Biosensor based on 3D graphene-supported Fe3O4 quantum dots as biomimetic enzyme for in situ detection of H2O2 released from living cells

2017 ◽  
Vol 244 ◽  
pp. 1037-1044 ◽  
Author(s):  
Yanan Zhao ◽  
Danqun Huo ◽  
Jing Bao ◽  
Mei Yang ◽  
Mei Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Living Cells ◽  
3D Graphene ◽  
In Situ Detection

In Situ Detection of Live Cancer Cells by Using Bioprobes Based on Au Nanoparticles

Langmuir ◽  
2008 ◽  
Vol 24 (21) ◽  
pp. 12112-12115 ◽  
Author(s):  
Jaemoon Yang ◽  
Kilho Eom ◽  
Eun-Kyung Lim ◽  
Jinsung Park ◽  
Yoonah Kang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Au Nanoparticles ◽  
In Situ Detection

In Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy

2003 ◽  
pp. 227-238
Author(s):  
Pei Tsai ◽  
Supatra Porasuphatana ◽  
Howard J. Halpern ◽  
Eugene D. Barth ◽  
Gerald M. Rosen
Keyword(s):  
Nitric Oxide ◽  
Epr Spectroscopy ◽  
Low Frequency ◽  
In Situ Detection

A multi-component Cu2O@FePO4 core–cage structure to jointly promote fast electron transfer toward the highly sensitive in situ detection of nitric oxide

Nanoscale ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 4471-4477 ◽  
Author(s):  
Yuhuan Zhang ◽  
Shi-Yu Lu ◽  
Zhuanzhuan Shi ◽  
Zhi Liang Zhao ◽  
Qian Liu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Electron Transfer ◽  
Fast Electron ◽  
Cage Structure ◽  
Highly Sensitive ◽  
In Situ Detection

Core–cage Cu2O@FePO4 nanocomposites for the highly sensitive in situ detection of nitric oxide.

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