A Cell-Surface-Anchored Ratiometric Fluorescent Probe for Extracellular pH Sensing

2014 ◽  
Vol 6 (17) ◽  
pp. 15329-15334 ◽  
Author(s):  
Guoliang Ke ◽  
Zhi Zhu ◽  
Wei Wang ◽  
Yuan Zou ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Cell Surface ◽  
Fluorescent Probe ◽  
Extracellular Ph ◽  
Ph Sensing ◽  
A Cell ◽  
Ratiometric Fluorescent Probe

A Cell-Surface-Specific Ratiometric Fluorescent Probe for Extracellular pH Sensing with Solid-State Fluorophore

ACS Sensors ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 2278-2285 ◽  
Author(s):  
Yan Yang ◽  
Mengchan Xia ◽  
Hansen Zhao ◽  
Sichun Zhang ◽  
Xinrong Zhang
Keyword(s):  
Solid State ◽  
Cell Surface ◽  
Fluorescent Probe ◽  
Extracellular Ph ◽  
Ph Sensing ◽  
A Cell ◽  
Ratiometric Fluorescent Probe

A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection

2016 ◽  
Vol 52 (50) ◽  
pp. 7818-7821 ◽  
Author(s):  
Le Ying ◽  
Nuli Xie ◽  
Yanjing Yang ◽  
Xiaohai Yang ◽  
Qifeng Zhou ◽  
...  
Keyword(s):  
Cell Surface ◽  
Extracellular Ph ◽  
A Cell ◽  
Ph Detection

A FRET-based sensor is anchored on the cell surface through streptavidin–biotin interactions.

A cell surface specific two-photon fluorescent probe for monitoring intercellular transmission of hydrogen sulfide

2017 ◽  
Vol 994 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Jia Fu ◽  
Hui-Wen Yao ◽  
Xiao-Yan Zhu ◽  
Xiao-Feng Guo ◽  
Hong Wang
Keyword(s):  
Hydrogen Sulfide ◽  
Cell Surface ◽  
Fluorescent Probe ◽  
Two Photon ◽  
A Cell

A novel ratiometric fluorescent probe from a hemicyanine derivative for detecting NAD(P)H in a cell microenvironment

2021 ◽  
Vol 13 (14) ◽  
pp. 1681-1686
Author(s):  
Jie Zhang ◽  
Zengchen Liu ◽  
Fengshou Tian ◽  
Yahong Chen
Keyword(s):  
Fluorescent Probe ◽  
Theoretical Basis ◽  
Cell Microenvironment ◽  
A Cell ◽  
Ratiometric Fluorescent Probe

In this paper, a ratiometric fluorescent probe for NAD(P)H derived from coumarin and hemicyanine was synthesized. The ratiometric fluorescent probe provided an important theoretical basis for sensing NAD(P)H.

scholarly journals Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jonas Mattisson ◽  
Marcus Danielsson ◽  
Maria Hammond ◽  
Hanna Davies ◽  
Caroline J. Gallant ◽  
...  
Keyword(s):  
Cell Surface ◽  
Single Cells ◽  
Surface Protein ◽  
Protein Abundance ◽  
Blood Leukocytes ◽  
Chromosome Y ◽  
Increased Risk ◽  
A Cell ◽  
Pseudoautosomal Regions ◽  
Male Specific

AbstractMosaic loss of chromosome Y (LOY) in immune cells is a male-specific mutation associated with increased risk for morbidity and mortality. The CD99 gene, positioned in the pseudoautosomal regions of chromosomes X and Y, encodes a cell surface protein essential for several key properties of leukocytes and immune system functions. Here we used CITE-seq for simultaneous quantification of CD99 derived mRNA and cell surface CD99 protein abundance in relation to LOY in single cells. The abundance of CD99 molecules was lower on the surfaces of LOY cells compared with cells without this aneuploidy in all six types of leukocytes studied, while the abundance of CD proteins encoded by genes located on autosomal chromosomes were independent from LOY. These results connect LOY in single cells with immune related cellular properties at the protein level, providing mechanistic insight regarding disease vulnerability in men affected with mosaic chromosome Y loss in blood leukocytes.

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