Induction of Myogenic Differentiation of Human Mesenchymal Stem Cells Cultured on Notch Agonist (Jagged-1) Modified Biodegradable Scaffold Surface

2014 ◽  
Vol 6 (3) ◽  
pp. 1652-1661 ◽  
Author(s):  
Feng Wen ◽  
Hui Kian Wong ◽  
Chor Yong Tay ◽  
Haiyang Yu ◽  
Huaqiong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Biodegradable Scaffold ◽  
Cells Cultured

Effect of Substrate Elasticity on In Vitro Aging of Human Mesenchymal Stem Cells

2012 ◽  
Vol 1498 ◽  
pp. 39-45
Author(s):  
Courtney E. LeBlon ◽  
Caitlin R. Fodor ◽  
Tony Zhang ◽  
Xiaohui Zhang ◽  
Sabrina S. Jedlicka
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Elastic Modulus ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Immunofluorescent Staining ◽  
Differentiation Potential ◽  
Gene And Protein Expression ◽  
The Impact

ABSTRACTHuman mesenchymal stem cells (hMSCs) were routinely cultured on tissue-culture polystyrene (TCPS) to investigate the in vitro aging and cell stiffening. hMSCs were also cultured on thermoplastic polyurethane (TPU), which is a biocompatible polymer with an elastic modulus of approximately 12.9MPa, to investigate the impact of substrate elastic modulus on cell stiffening and differentiation potential. Cells were passaged over several generations on each material. At each passage, cells were subjected to osteogenic and myogenic differentiation. Local cell elastic modulus was measured at every passage using atomic force microscopy (AFM) indentation. Gene and protein expression was examined using qRT-PCR and immunofluorescent staining, respectively, for osteogenic and myogenic markers. Results show that the success of myogenic differentiation is highly reliant on the elastic modulus of the undifferentiated cells. The success of osteogenic differentiations is most likely somewhat dependent on the cell elastic modulus, as differentiations were more successful in earlier passages, when cells were softer.

Gene Expression of Human Mesenchymal Stem Cells Cultured on Titanium Dental Implant Surfaces

2016 ◽  
Vol 27 (3) ◽  
pp. 712-717 ◽  
Author(s):  
Antonio Barone ◽  
Paolo Toti ◽  
Dario Bertossi ◽  
Simone Marconcini ◽  
Daniele De Santis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Implant ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Dental Implant ◽  
Cells Cultured
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