An Optical Sensing Strategy Leading to In Situ Monitoring of the Degradation of Mesoporous Magnetic Supraparticles in Cells

2013 ◽  
Vol 5 (23) ◽  
pp. 12329-12339 ◽  
Author(s):  
Dian Li ◽  
Yuting Zhang ◽  
Peng Yang ◽  
Meng Yu ◽  
Jia Guo ◽  
...  
Keyword(s):  
Optical Sensing ◽  
In Situ Monitoring ◽  
In Cells

Correction to An Optical Sensing Strategy Leading to In Situ Monitoring of the Degradation of Mesoporous Magnetic Supraparticles in Cells

2014 ◽  
Vol 6 (4) ◽  
pp. 3039-3039
Author(s):  
Dian Li ◽  
Yuting Zhang ◽  
Peng Yang ◽  
Meng Yu ◽  
Jia Guo ◽  
...  
Keyword(s):  
Optical Sensing ◽  
In Situ Monitoring ◽  
In Cells

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

scholarly journals Development of an electrochemical sensor for in-situ monitoring of reactive species produced by cold physical plasma

2021 ◽  
Vol 326 ◽  
pp. 129007
Author(s):  
Zahra Nasri ◽  
Giuliana Bruno ◽  
Sander Bekeschus ◽  
Klaus-Dieter Weltmann ◽  
Thomas von Woedtke ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Reactive Species ◽  
In Situ Monitoring ◽  
Physical Plasma

In Situ Monitoring of Polaron Localization and Cell Activity with Charge Accumulation Spectroscopy

2021 ◽  
pp. 2105799
Author(s):  
Yu Zhang ◽  
Li Yang ◽  
Jintao Wang ◽  
Wangying Xu ◽  
Qiming Zeng ◽  
...  
Keyword(s):  
Cell Activity ◽  
In Situ Monitoring ◽  
Charge Accumulation
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