Synthesis of Fe3O4/Graphene/TiO2 Composites for the Highly Selective Enrichment of Phosphopeptides from Biological Samples

2013 ◽  
Vol 5 (15) ◽  
pp. 7330-7334 ◽  
Author(s):  
Jin Lu ◽  
Chunhui Deng ◽  
Xiangmin Zhang ◽  
Pengyuan Yang
2016 ◽  
Vol 4 (36) ◽  
pp. 6125-6133 ◽  
Author(s):  
Xiangyang An ◽  
Xiwen He ◽  
Langxing Chen ◽  
Yukui Zhang

A facile and efficient method was developed to synthesize boronic acid polymer brushes immobilized on magnetic graphene oxide for the selective enrichment of glycoproteins from complex biological samples via surface initiated atom transfer radical polymerization (SI-ATRP).


2017 ◽  
Vol 9 (3) ◽  
pp. 443-449 ◽  
Author(s):  
Yiman Zhao ◽  
Lingyi Zhang ◽  
Zhanying Chu ◽  
Zhichao Xiong ◽  
Weibing Zhang

For in-depth analysis of phosphorylated proteomics, the highly sensitive and selective capture of phosphopeptides from intricate biological samples is extremely significant.


The Analyst ◽  
2021 ◽  
Author(s):  
Yameng Zhao ◽  
Lingyi Zhang ◽  
Licheng Cao ◽  
Lei Zhang ◽  
Weibing Zhang

Highly effective enrichment of endogenous phosphopeptides from complex biological samples is an essential and crucial theme in the analysis of phosphopeptidomics.


Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.


Author(s):  
C. F. Oster

Although ultra-thin sectioning techniques are widely used in the biological sciences, their applications are somewhat less popular but very useful in industrial applications. This presentation will review several specific applications where ultra-thin sectioning techniques have proven invaluable.The preparation of samples for sectioning usually involves embedding in an epoxy resin. Araldite 6005 Resin and Hardener are mixed so that the hardness of the embedding medium matches that of the sample to reduce any distortion of the sample during the sectioning process. No dehydration series are needed to prepare our usual samples for embedding, but some types require hardening and staining steps. The embedded samples are sectioned with either a prototype of a Porter-Blum Microtome or an LKB Ultrotome III. Both instruments are equipped with diamond knives.In the study of photographic film, the distribution of the developed silver particles through the layer is important to the image tone and/or scattering power. Also, the morphology of the developed silver is an important factor, and cross sections will show this structure.


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