Label-Free Detection of Staphylococcus aureus Captured on Immutable Ligand Arrays

2013 ◽  
Vol 5 (13) ◽  
pp. 6404-6411 ◽  
Author(s):  
Avijit K. Adak ◽  
J. William Boley ◽  
David P. Lyvers ◽  
George T. Chiu ◽  
Philip S. Low ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Label Free ◽  
Label Free Detection

Integrated microfluidic platform with electrohydrodynamic focusing and a carbon-nanotube-based field-effect transistor immunosensor for continuous, selective, and label-free quantification of bacteria

Lab on a Chip ◽  
2021 ◽  
Vol 21 (1) ◽  
pp. 184-195
Author(s):  
Chang-Ho Han ◽  
Jaesung Jang
Keyword(s):  
Staphylococcus Aureus ◽  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Label Free ◽  
Microfluidic Platform ◽  
Label Free Quantification ◽  
Label Free Detection ◽  
Effect Transistor ◽  
Free Quantification

A microfluidic carbon-nanotube-based field-effect transistor immunosensor equipped with electrohydrodynamic focusing for continuous and label-free detection of flowing Staphylococcus aureus particles.

scholarly journals Development of Highly Sensitive Immunosensor for Detection of Staphylococcus aureus Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 446
Author(s):  
En Han ◽  
Yun Zhang ◽  
Jianrong Cai ◽  
Xinai Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Catalytic Performance ◽  
High Specific Surface Area ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Label Free ◽  
Catalytic Current ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Growth Method

The rapid and sensitive detection of Staphylococcus aureus (S. aureus) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functionalized multi-walled carbon nanotubes (MWCNTs-AuPdPt) as the signal amplification platform was designed for the label-free detection of S. aureus. The nanocomposite of MWCNTs-AuPdPt was prepared by an in situ growth method of loading AuPdPt trimetallic nanoparticles on the surface of MWCNTs. The synthesized MWCNTs-AuPdPt featured good conductivity and superior catalytic performance for hydrogen peroxide. The nanocomposite of MWCNTs-AuPdPt with good biocompatibility and high specific surface area was further functionalized by anti-S. aureus antibodies. The immobilized antibodies could efficiently capture S. aureus to the modified electrode by an immune reaction, which resulted in the change of catalytic current intensity to realize the sensitive detection of S. aureus. The designed immunosensor could detect S. aureus in a linear range from 1.1 × 102 to 1.1 × 107 CFU mL−1 with a low detection limit of 39 CFU mL−1. Additionally, the proposed immunosensor was successfully applied to determine S. aureus in actual samples with acceptable results. This strategy provided a promising platform for highly sensitive determination of S. aureus and other pathogens in food products.

Label-free detection of Staphylococcus aureus in skin using real-time potentiometric biosensors based on carbon nanotubes and aptamers

2012 ◽  
Vol 31 (1) ◽  
pp. 226-232 ◽  
Author(s):  
Gustavo A. Zelada-Guillén ◽  
José Luis Sebastián-Avila ◽  
Pascal Blondeau ◽  
Jordi Riu ◽  
F. Xavier Rius
Keyword(s):  
Carbon Nanotubes ◽  
Staphylococcus Aureus ◽  
Real Time ◽  
Label Free ◽  
Label Free Detection

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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