Fluorescent Detection of Lead in Environmental Water and Urine Samples Using Enzyme Mimics of Catechin-Synthesized Au Nanoparticles

2013 ◽  
Vol 5 (4) ◽  
pp. 1503-1509 ◽  
Author(s):  
Yan-Shiuan Wu ◽  
Fan-Feng Huang ◽  
Yang-Wei Lin
Keyword(s):  
Au Nanoparticles ◽  
Environmental Water ◽  
Urine Samples ◽  
Fluorescent Detection ◽  
Enzyme Mimics

scholarly journals Metal Accumulation Using a Bacterium (K-142) Identified from Environmental Microorganisms by the Screening of Au Nanoparticles Synthesis

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4922
Author(s):  
Yiting Li ◽  
Michio Suzuki
Keyword(s):  
Au Nanoparticles ◽  
Deposition Efficiency ◽  
Environmental Water ◽  
Sustainable Society ◽  
Pbs Nanoparticles ◽  
Biological Water ◽  
Use Of Technology ◽  
Bacillus Genus ◽  
Opening Up ◽  
Observation Results

The use of technology that uses organisms to synthesize metal nanoparticles is necessary to maintain a sustainable society. In this study, we investigated and screened the microorganisms isolated from environmental water by quantifying the reproducibility of synthetic Au nanoparticles and the ability of large amount synthesis. The microorganism (K-142) of the Bacillus genus showed the best activity in the investigation. K-142 can also synthesize Ag, CdS and PbS nanoparticles, and the deposition efficiency of Ag, Al, Cd, Cu, and Pb was about 64.8–99.2%. According to the observation results under the microscope after fluorescent staining, K-142 could survive after being treated with 0.5 mM metal solution for 24 h. Therefore, it is expected that K-142, which is easy to cultivate, would also have a high ability to reduce and deposit metal substances. K-142 can be applied to the concentration and recovery of heavy metals in environmental water, thereby opening up channels for biological water purification.

Enzyme Mimics of Au/Ag Nanoparticles for Fluorescent Detection of Acetylcholine

2012 ◽  
Vol 84 (22) ◽  
pp. 9706-9712 ◽  
Author(s):  
Chen-I Wang ◽  
Wen-Tsen Chen ◽  
Huan-Tsung Chang
Keyword(s):  
Ag Nanoparticles ◽  
Fluorescent Detection ◽  
Enzyme Mimics

Turn-on fluorescent detection of captopril in urine samples based on hydrophilic hydroxypropyl β-cyclodextrin polymer

2018 ◽  
Vol 410 (28) ◽  
pp. 7373-7384 ◽  
Author(s):  
Yi Shi ◽  
Jun Peng ◽  
Xiaoyan Meng ◽  
Tao Huang ◽  
Jingyi Zhang ◽  
...  
Keyword(s):  
Urine Samples ◽  
Fluorescent Detection ◽  
Turn On ◽  
Cyclodextrin Polymer

scholarly journals Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection

2013 ◽  
Vol 11 (12) ◽  
pp. 2076-2087 ◽  
Author(s):  
Sylwia Magiera ◽  
Weronika Adolf ◽  
Irena Baranowska
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Urine ◽  
High Performance ◽  
Solid Phase ◽  
Multiple Reaction Monitoring ◽  
Urine Samples ◽  
Fluorescent Detection ◽  
Human Urine Samples

AbstractA sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng mL−1 to 200 ng mL−1 for the analysed compounds. The limit of quantification varied from 14.2 ng mL−1 to 24.2 ng mL−1. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease.

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