Preparation of Superparamagnetic Fe3O4@Alginate/Chitosan Nanospheres for Candida rugosa lipase Immobilization and Utilization of Layer-by-Layer Assembly to Enhance the Stability of Immobilized Lipase

2012 ◽  
Vol 4 (10) ◽  
pp. 5169-5178 ◽  
Author(s):  
Xiao Liu ◽  
Xia Chen ◽  
Yanfeng Li ◽  
Xinyu Wang ◽  
Xiaomeng Peng ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Layer By Layer ◽  
Lipase Immobilization ◽  
Layer By Layer Assembly ◽  
The Stability ◽  
Layer Assembly

scholarly journals Preparation and Candida rugosa Lipase Immobilization on Nylon-6 Grafted and Aminated (Polyvinyl Benzyl Chloride) Microfibers

2019 ◽  
Vol 14 (2) ◽  
pp. 369
Author(s):  
Nur Iilani Abd Halin ◽  
Maan Fahmi Rashid Al-Khatib ◽  
Hamzah Mohd. Salleh ◽  
Mohamed Mahmoud Nasef
Keyword(s):  
Benzyl Chloride ◽  
Immobilized Lipase ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Nylon 6 ◽  
Covalent Attachment ◽  
Effect Of Temperature ◽  
P Value ◽  
Lipase Immobilization ◽  
The Stability

This paper demonstrates a simplified procedure for the preparation of a nylon-6 microfibers based support for the immobilization of Candida rugosa lipase via covalent attachment to enhance the stability and reusability of lipase. The preparation of the support was done by radiation induced graft copolymerization (RIGC) of vinyl benzyl chloride (VBC) onto nylon-6 microfibers followed by amination with ethanolamine to facilitate the immobilization of lipase. Fourier transfer infra red (FTIR) and scanning electron microscope (SEM) were used to study the chemical and physical changes following grafting, amination and immobilization. Response surface methodology (RSM) was applied for the optimization of lipase immobilization on the aminated microfibers. The optimization parameters were incubation time, pH, and lipase concentration. Moreover, this study investigated the effect of temperature, pH, and storage stability and reusability on the lipase in its immobilized and free forms. The developed model from RSM showed an R2 value of 0.9823 and P-value < 0.001 indicating that the model is significant. The optimum temperatures for both immobilized and free lipases were 45 °C, whereas the best pH values for lipase activity were at pH 8 and pH 7, respectively. This study also identifies values for KM and Vmax for both immobilized and free lipase accordingly. Based on the results, immobilized lipase had significantly improved the stability and reusability of lipase compared to that in free forms. Copyright © 2019 BCREC Group. All rights reserved 

scholarly journals Novel approaches to immobilize Candida rugosa lipase on nanocomposite membranes prepared by covalent attachment of magnetic nanoparticles on poly acrylonitrile membrane

RSC Advances ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 4561-4570 ◽  
Author(s):  
Marzieh Aghababaie ◽  
Masoud Beheshti ◽  
Abdol-Khalegh Bordbar ◽  
Amir Razmjou
Keyword(s):  
Magnetic Nanoparticles ◽  
Immobilized Lipase ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Covalent Attachment ◽  
Lipase Immobilization ◽  
Nanocomposite Membranes ◽  
Novel Approaches ◽  
Novel Methods ◽  
Poly Acrylonitrile

Novel methods have been developed for lipase immobilization on poly acrylonitrile (PAN) membranes to increase the activity and stability of the immobilized lipase.

Complex formation of modified chitosan and carboxymethyl cellulose and its effect on paper properties

TAPPI Journal ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 29-35 ◽  
Author(s):  
PEDRAM FATEHI ◽  
LIYING QIAN ◽  
RATTANA KITITERAKUN ◽  
THIRASAK RIRKSOMBOON ◽  
HUINING XIAO
Keyword(s):  
Carboxymethyl Cellulose ◽  
Polymer System ◽  
Layer By Layer ◽  
Paper Strength ◽  
Anionic Polymer ◽  
Paper Properties ◽  
Modified Chitosan ◽  
Layer By Layer Assembly ◽  
Polyelectrolyte Titration ◽  
Layer Assembly

The application of an oppositely charged dual polymer system is a promising approach to enhance paper strength. In this work, modified chitosan (MCN), a cationic polymer, and carboxymethyl cellulose (CMC), an anionic polymer, were used sequentially to improve paper strength. The adsorption of MCN on cellulose fibers was analyzed via polyelectrolyte titration. The formation of MCN/CMC complex in water and the deposition of this complex on silicon wafers were investigated by means of atomic force microscope and quasi-elastic light scattering techniques. The results showed that paper strength was enhanced slightly with a layer-by-layer assembly of the polymers. However, if the washing stage, which was required for layer-by-layer assembly, was eliminated, the MCN/CMC complex was deposited on fibers more efficiently, and the paper strength was improved more significantly. The significant improvement was attributed to the extra development of fiber bonding, confirmed further by scanning electron microscope observation of the bonding area of fibers treated with or without washing. However, the brightness of papers was somewhat decreased by the deposition of the complex on fibers. Higher paper strength also was achieved using rapid drying rather than air drying.

Stabilization of starch-based microgel-lysozyme complexes using a layer-by-layer assembly technique

2017 ◽  
Vol 214 ◽  
pp. 213-217 ◽  
Author(s):  
Bao Zhang ◽  
Yi Pan ◽  
Hanqing Chen ◽  
Tengfei Liu ◽  
Han Tao ◽  
...  
Keyword(s):  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Assembly Technique ◽  
Layer Assembly

Layer-by-layer assembly of hemoglobin and gold nanoparticles for enhancing the ability of oxygen carrying

2016 ◽  
Vol 363 ◽  
pp. 566-571 ◽  
Author(s):  
Lijun Sun ◽  
Xiaojun Liu ◽  
Qiaoling Sun ◽  
Meng Cai ◽  
Jiajing Zhou ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Layer Assembly
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