Light-Induced Covalent Immobilization of Monolayers of Magnetic Nanoparticles on Hydrogen-Terminated Silicon

2010 ◽  
Vol 2 (10) ◽  
pp. 2789-2796 ◽  
Author(s):  
Gyu Leem ◽  
Shishan Zhang ◽  
Andrew C. Jamison ◽  
Eduard Galstyan ◽  
Irene Rusakova ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Covalent Immobilization

Site-specific, covalent immobilization of an engineered enterokinase onto magnetic nanoparticles through transglutaminase-catalyzed bioconjugation

2019 ◽  
Vol 177 ◽  
pp. 506-511 ◽  
Author(s):  
Jing-Hong Wang ◽  
Ming-Ze Tang ◽  
Xiao-Tian Yu ◽  
Chong-Mei Xu ◽  
Hong-Ming Yang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Covalent Immobilization ◽  
Site Specific

Covalent Immobilization of Penicillin G Acylase onto Fe3O4@Chitosan Magnetic Nanoparticles

2016 ◽  
Vol 26 (5) ◽  
pp. 829-836 ◽  
Author(s):  
Xiao-Min Ling ◽  
Xiang-Yu Wang ◽  
Ping Ma ◽  
Yi Yang ◽  
Jie-Mei Qin ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Covalent Immobilization ◽  
Penicillin G Acylase ◽  
Penicillin G

scholarly journals Covalent immobilization of coagulation factor VIII on magnetic nanoparticles for aptamer development

2018 ◽  
Vol 16 (3) ◽  
pp. 161-170 ◽  
Author(s):  
Maryam Tabarzad ◽  
Zeinab Sharafi ◽  
Jaber Javidi
Keyword(s):  
Magnetic Nanoparticles ◽  
Factor Viii ◽  
Coagulation Factor ◽  
Silica Coating ◽  
Covalent Immobilization ◽  
Coagulation Factor Viii ◽  
Aptamer Selection ◽  
Selection Processes ◽  
Reactive Groups

Introduction: Magnetic nanoparticles (MNPs) are one of the most useful particulate systems in analytical applications such as specific aptamer selection. Proteins are the most noted targets of aptamer selection. Generally, covalently immobilized protein coated MNPs are more stable structures. Methods: In this study, coagulation factor VIII (FVIII) was immobilized on MNPs. A silica coating provided isocyanate functional groups was considered to interact covalently with reactive groups of the protein, resulting in a stable protein immobilization. The reactions was run in dried toluene. At end, these MNPs were applied for affinity determination of a previously selected FVIII specific aptamers. Results: Immobilization of 1 mg FVIII (~ 3 nmol) on 5 mg particles was achieved with no significant particle aggregation. Using a fluorescence-based method, affinity measurement resulted in a calculated dissociation constant of 120 ± 5.6 nM for the FVIII-specific aptamer to the FVIII-coated MNPs. Conclusion: The final product could be a suitable protein-coated solid support for magnetic-based aptamer selection processes.

pHLIP-modified magnetic nanoparticles for targeting acidic diseased tissue

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60196-60199 ◽  
Author(s):  
A. M. Demin ◽  
A. G. Pershina ◽  
K. V. Nevskaya ◽  
L. V. Efimova ◽  
N. N. Shchegoleva ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Covalent Immobilization ◽  
Modified Magnetic Nanoparticles

Covalent immobilization of a pH-low insertion peptide (pHLIP) onto Fe3O4 magnetic nanoparticles was carried out resulting in the formation of MRI-visible material able to specifically accumulate in acidic damaged tissue.

scholarly journals Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3968
Author(s):  
Arpita Saha ◽  
Hamdi Ben Halima ◽  
Abhishek Saini ◽  
Juan Gallardo-Gonzalez ◽  
Nadia Zine ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Artificial Saliva ◽  
Coupling Reaction ◽  
Interleukin 10 ◽  
Covalent Immobilization ◽  
Precipitation Method ◽  
Necrosis Factor Alpha ◽  
The Matrix ◽  
Co Precipitation ◽  
Detection Of Biomarkers

Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved.

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