scholarly journals Optimizing Oleaginous Yeast Cell Factories for Flavonoids and Hydroxylated Flavonoids Biosynthesis

2019 ◽  
Vol 8 (11) ◽  
pp. 2514-2523 ◽  
Author(s):  
Yongkun Lv ◽  
Monireh Marsafari ◽  
Mattheos Koffas ◽  
Jingwen Zhou ◽  
Peng Xu
Keyword(s):  
Yeast Cell ◽  
Oleaginous Yeast ◽  
Cell Factories ◽  
Flavonoids Biosynthesis

scholarly journals Optimizing oleaginous yeast cell factories for flavonoids and hydroxylated flavonoids biosynthesis

2019 ◽  
Author(s):  
Yongkun Lv ◽  
Mattheos Koffas ◽  
Jingwen Zhou ◽  
Peng Xu
Keyword(s):  
Oleaginous Yeast ◽  
Heterologous Production ◽  
Modular Construction ◽  
Metabolic Potential ◽  
Cell Factories ◽  
Plant Extraction ◽  
Health Promoting ◽  
Product Manufacturing ◽  
Hydrophobic Lipid ◽  
Flavonoids Biosynthesis

AbstractPlants possess myriads of secondary metabolites with a broad spectrum of health-promoting benefits. Up to date, plant extraction is still the primary route to produce high-value natural products, which inherently suffers from economics and scalability issues. Heterologous production in microbial host is considered as a feasible approach to overcoming these limitations. Flavonoid and its hydroxylated derivatives represent a diversified family of bioactive compounds, most prominently known as antioxidant and anti-aging agents. Oleaginous yeast is rich in hydrophobic lipid bodies and spatially-organized organelles, which provides the ideal environment for the regioselectivity and stereoselectivity of many plant-specific enzymes. In this report, we validated thatY. lipolyticais a superior platform for heterologous production of high-value flavonoids and hydroxylated flavonoids. By modular construction and characterization, we determined the rate-limiting steps for efficient flavonoids biosynthesis inY. lipolytica. We evaluated various precursor pathways and unleashed the metabolic potential ofY. lipolyticato produce flavonoids, including the supply of acetyl-CoA, malonyl-CoA and chorismate. Coupled with the optimized chalcone synthase module and the hydroxylation module, our engineered strain produced 252.4 mg/L naringenin, 134.2 mg/L eriodictyol and 110.5 mg/L taxifolin from glucose. Collectively, these findings demonstrate our ability to harness oleaginous yeast as microbial workhorse to expand nature’s biosynthetic potential, enabling us to bridge the gap between drug discovery and natural product manufacturing.

Mannosylerythritol Lipid Production from Oleaginous Yeast Cell Lysate by Moesziomyces aphidis

2020 ◽  
Vol 16 (4) ◽  
pp. 222-232
Author(s):  
Veerle Akkermans ◽  
Ruben Verstraete ◽  
Caroline Braem ◽  
Jolien D'aes ◽  
Jan Dries
Keyword(s):  
Yeast Cell ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Mannosylerythritol Lipid ◽  
Cell Lysate

scholarly journals Enhancement of Astaxanthin Biosynthesis in Oleaginous Yeast Yarrowia lipolytica via Microalgal Pathway

2019 ◽  
Vol 7 (10) ◽  
pp. 472 ◽  
Author(s):  
Larissa Ribeiro Ramos Tramontin ◽  
Kanchana Rueksomtawin Kildegaard ◽  
Suresh Sudarsan ◽  
Irina Borodina
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Small Scale ◽  
Cell Factory ◽  
Standard Equipment ◽  
Cell Factories ◽  
Dry Cell Weight ◽  
Astaxanthin Production ◽  
Yeast Yarrowia Lipolytica ◽  
Β Carotene

Astaxanthin is a high-value red pigment and antioxidant used by pharmaceutical, cosmetics, and food industries. The astaxanthin produced chemically is costly and is not approved for human consumption due to the presence of by-products. The astaxanthin production by natural microalgae requires large open areas and specialized equipment, the process takes a long time, and results in low titers. Recombinant microbial cell factories can be engineered to produce astaxanthin by fermentation in standard equipment. In this work, an oleaginous yeast Yarrowia lipolytica was engineered to produce astaxanthin at high titers in submerged fermentation. First, a platform strain was created with an optimised pathway towards β-carotene. The platform strain produced 331 ± 66 mg/L of β-carotene in small-scale cultivation, with the cellular content of 2.25% of dry cell weight. Next, the genes encoding β-ketolase and β-hydroxylase of bacterial (Paracoccus sp. and Pantoea ananatis) and algal (Haematococcus pluvialis) origins were introduced into the platform strain in different copy numbers. The resulting strains were screened for astaxanthin production, and the best strain, containing algal β-ketolase and β-hydroxylase, resulted in astaxanthin titer of 44 ± 1 mg/L. The same strain was cultivated in controlled bioreactors, and a titer of 285 ± 19 mg/L of astaxanthin was obtained after seven days of fermentation on complex medium with glucose. Our study shows the potential of Y. lipolytica as the cell factory for astaxanthin production.

Industrial systems biology and its impact on synthetic biology of yeast cell factories

2015 ◽  
Vol 113 (6) ◽  
pp. 1164-1170 ◽  
Author(s):  
Eugene Fletcher ◽  
Anastasia Krivoruchko ◽  
Jens Nielsen
Keyword(s):  
Systems Biology ◽  
Synthetic Biology ◽  
Yeast Cell ◽  
Industrial Systems ◽  
Cell Factories

scholarly journals Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Santosh Pandit ◽  
Oliver Konzock ◽  
Kirsten Leistner ◽  
VRSS Mokkapati ◽  
Alessandra Merlo ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Carbon Sources ◽  
Environmentally Friendly ◽  
Cost Effective ◽  
Intracellular Lipids ◽  
Cell Factories ◽  
Coated Nanoparticles ◽  
Cellular Lipids ◽  
Extraction Processes ◽  
Available Carbon

AbstractEngineering of microbial cells to produce high value chemicals is rapidly advancing. Yeast, bacteria and microalgae are being used to produce high value chemicals by utilizing widely available carbon sources. However, current extraction processes of many high value products from these cells are time- and labor-consuming and require toxic chemicals. This makes the extraction processes detrimental to the environment and not economically feasible. Hence, there is a demand for the development of simple, effective, and environmentally friendly method for the extraction of high value chemicals from these cell factories. Herein, we hypothesized that atomically thin edges of graphene having ability to interact with hydrophobic materials, could be used to extract high value lipids from cell factories. To achieve this, array of axially oriented graphene was deposited on iron nanoparticles. These coated nanoparticles were used to facilitate the release of intracellular lipids from Yarrowia lipolytica cells. Our treatment process can be integrated with the growth procedure and achieved the release of 50% of total cellular lipids from Y. lipolytica cells. Based on this result, we propose that nanoparticles coated with axially oriented graphene could pave efficient, environmentally friendly, and cost-effective way to release intracellular lipids from yeast cell factories.

scholarly journals Synthesizing ginsenoside Rh2 in Saccharomyces cerevisiae cell factory at high-efficiency

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Pingping Wang ◽  
Wei Wei ◽  
Wei Ye ◽  
Xiaodong Li ◽  
Wenfang Zhao ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Batch Fermentation ◽  
Cell Factory ◽  
Ginsenoside Rh2 ◽  
Fed Batch ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Fed Batch Fermentation ◽  
Shake Flasks ◽  
Produce Plant

AbstractSynthetic biology approach has been frequently applied to produce plant rare bioactive compounds in microbial cell factories by fermentation. However, to reach an ideal manufactural efficiency, it is necessary to optimize the microbial cell factories systemically by boosting sufficient carbon flux to the precursor synthesis and tuning the expression level and efficiency of key bioparts related to the synthetic pathway. We previously developed a yeast cell factory to produce ginsenoside Rh2 from glucose. However, the ginsenoside Rh2 yield was too low for commercialization due to the low supply of the ginsenoside aglycone protopanaxadiol (PPD) and poor performance of the key UDP-glycosyltransferase (UGT) (biopart UGTPg45) in the final step of the biosynthetic pathway. In the present study, we constructed a PPD-producing chassis via modular engineering of the mevalonic acid pathway and optimization of P450 expression levels. The new yeast chassis could produce 529.0 mg/L of PPD in shake flasks and 11.02 g/L in 10 L fed-batch fermentation. Based on this high PPD-producing chassis, we established a series of cell factories to produce ginsenoside Rh2, which we optimized by improving the C3–OH glycosylation efficiency. We increased the copy number of UGTPg45, and engineered its promoter to increase expression levels. In addition, we screened for more efficient and compatible UGT bioparts from other plant species and mutants originating from the direct evolution of UGTPg45. Combining all engineered strategies, we built a yeast cell factory with the greatest ginsenoside Rh2 production reported to date, 179.3 mg/L in shake flasks and 2.25 g/L in 10 L fed-batch fermentation. The results set up a successful example for improving yeast cell factories to produce plant rare natural products, especially the glycosylated ones.

scholarly journals Engineering tolerance to industrially relevant stress factors in yeast cell factories

2017 ◽  
Vol 17 (4) ◽  
Author(s):  
Quinten Deparis ◽  
Arne Claes ◽  
Maria R. Foulquié-Moreno ◽  
Johan M. Thevelein
Keyword(s):  
Yeast Cell ◽  
Stress Factors ◽  
Cell Factories
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