Precursor Supply for Erythromycin Biosynthesis: Engineering of Propionate Assimilation Pathway Based on Propionylation Modification

2019 ◽  
Vol 8 (2) ◽  
pp. 371-380 ◽  
Author(s):  
Di You ◽  
Miao-Miao Wang ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Erythromycin Biosynthesis ◽  
Precursor Supply

scholarly journals Matching the supply of bacterial nutrients to the nutritional demand of the animal host

2014 ◽  
Vol 281 (1791) ◽  
pp. 20141163 ◽  
Author(s):  
Calum W. Russell ◽  
Anton Poliakov ◽  
Meena Haribal ◽  
Georg Jander ◽  
Klaas J. van Wijk ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Acyrthosiphon Pisum ◽  
Feedback Inhibition ◽  
Essential Amino Acids ◽  
Host Cells ◽  
Host Proteins ◽  
Buchnera Aphidicola ◽  
Symbiotic Microorganisms ◽  
Precursor Supply ◽  
Host Regulation

Various animals derive nutrients from symbiotic microorganisms with much-reduced genomes, but it is unknown whether, and how, the supply of these nutrients is regulated. Here, we demonstrate that the production of essential amino acids (EAAs) by the bacterium Buchnera aphidicola in the pea aphid Acyrthosiphon pisum is elevated when aphids are reared on diets from which that EAA are omitted, demonstrating that Buchnera scale EAA production to host demand. Quantitative proteomics of bacteriocytes (host cells bearing Buchnera ) revealed that these metabolic changes are not accompanied by significant change in Buchnera or host proteins, suggesting that EAA production is regulated post-translationally. Bacteriocytes in aphids reared on diet lacking the EAA methionine had elevated concentrations of both methionine and the precursor cystathionine, indicating that methionine production is promoted by precursor supply and is not subject to feedback inhibition by methionine. Furthermore, methionine production by isolated Buchnera increased with increasing cystathionine concentration. We propose that Buchnera metabolism is poised for EAA production at certain maximal rates, and the realized release rate is determined by precursor supply from the host. The incidence of host regulation of symbiont nutritional function via supply of key nutritional inputs in other symbioses remains to be investigated.

Variation of surface morphology with precursor supply ratio in MOVPE CdTe layers

1993 ◽  
Vol 128 (1-4) ◽  
pp. 613-616 ◽  
Author(s):  
Touati Ferid ◽  
Mitsuru Ekawa ◽  
Kazuhito Yasuda ◽  
Akikazu Tanaka ◽  
Manabu Saji
Keyword(s):  
Surface Morphology ◽  
Precursor Supply

Understanding in vivo carbon precursor supply for fatty acid synthesis in leaf tissue

2000 ◽  
Vol 22 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Xiaoming Bao ◽  
Manfred Focke ◽  
Mike Pollard ◽  
John Ohlrogge
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Leaf Tissue ◽  
Acid Synthesis ◽  
Carbon Precursor ◽  
Precursor Supply

Effect of Precursor Supply on (100) and (001) Orientations of α-Al2O3 Film Prepared by Laser CVD

2012 ◽  
Vol 508 ◽  
pp. 3-6 ◽  
Author(s):  
Kadokura Hokuto ◽  
Akihiko Ito ◽  
Teiichi Kimura ◽  
Takashi Goto
Keyword(s):  
Cross Section ◽  
Vapor Deposition ◽  
Gas Flow ◽  
Chemical Vapor ◽  
Gas Flow Rate ◽  
Laser Chemical Vapor Deposition ◽  
Evaporation Temperature ◽  
Oxygen Gas ◽  
Laser Cvd ◽  
Precursor Supply

Α-Al2O3 Films Were Prepared by Laser Chemical Vapor Deposition and the Effects of Precursor Evaporation Temperature (Tvap) and Oxygen Gas Flow Rate (FRo) on Phase and Orientation of Al2o3 Films Were Investigated. at Tvap = 413 K, (100)-Oriented α-Al2O3 and θ-Al2O3 Were Codeposited. the Amount of θ-Al2O3 Increased with Increasing FRo. at Tvap = 433–443 K, α-Al2O3 Films Showed a (001) Orientation. (100)- and (001)-Oriented α-Al2O3 Films Had a Rectangular- and Hexagonal-Shaped Grains, Respectively, and Showed a Columnar in Cross Section. Grain Size of (100)- and (001)-Oriented α-Al2O3 Films Decreased from 10 to 2 μm with Increasing FRo from 0.085 to 0.85 Pa m3 s−1. Deposition Rate Increased from 100 to 300 μm h−1 with Increasing Tvap from 413 to 443 K.

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