Incorporation of a Synthetic Amino Acid into dCas9 Improves Control of Gene Silencing

2019 ◽  
Vol 8 (2) ◽  
pp. 216-222 ◽  
Author(s):  
Balwina Koopal ◽  
Aleksander J. Kruis ◽  
Nico J. Claassens ◽  
Franklin L. Nobrega ◽  
John van der Oost
Keyword(s):  
Amino Acid ◽  
Gene Silencing ◽  
Synthetic Amino Acid

scholarly journals Advancing peptide siRNA-carrier designs through L/D-amino acid stereochemical modifications to enhance gene silencing

2021 ◽  
Author(s):  
Charles E. Holjencin ◽  
Colton R. Feinberg ◽  
Travis Hedrick ◽  
Gregory Halsey ◽  
Robert D. Williams ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Silencing

Effect of Glycine and Serine in Synthetic Amino Acid Diets upon Glycine and Serine Metabolism in Chicks

1974 ◽  
Vol 104 (8) ◽  
pp. 1018-1023 ◽  
Author(s):  
C. N. Coon ◽  
L. W. Luther ◽  
J. R. Couch
Keyword(s):  
Amino Acid ◽  
Synthetic Amino Acid ◽  
Serine Metabolism

scholarly journals WRAP-based nanoparticles for siRNA delivery: a SAR study and a comparison with lipid-based transfection reagents

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Karidia Konate ◽  
Emilie Josse ◽  
Milana Tasic ◽  
Karima Redjatti ◽  
Gudrun Aldrian ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Gene Silencing ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Sirna Delivery ◽  
Cell Penetrating Peptides ◽  
Sirna Transfection ◽  
Arginine Residues ◽  
Sar Study

AbstractRecently, we designed novel amphipathic cell-penetrating peptides, called WRAP, able to transfer efficiently siRNA molecules into cells. In order to gain more information about the relationship between amino acid composition, nanoparticle formation and cellular internalization of these peptides composed of only three amino acids (leucine, arginine and tryptophan), we performed a structure–activity relationship (SAR) study. First, we compared our WRAP1 and WRAP5 peptides with the C6M1 peptide also composed of the same three amino acids and showing similar behaviors in siRNA transfection. Afterwards, to further define the main determinants in the WRAP activity, we synthesized 13 new WRAP analogues harboring different modifications like the number and location of leucine and arginine residues, the relative location of tryptophan residues, as well as the role of the α-helix formation upon proline insertions within the native WRAP sequence. After having compared the ability of these peptides to form peptide-based nanoparticles (PBNs) using different biophysical methods and to induce a targeted gene silencing in cells, we established the main sequential requirements of the amino acid composition of the WRAP peptide. In addition, upon measuring the WRAP-based siRNA transfection ability into cells compared to several non-peptide transfection agents available on the markets, we confirmed that WRAP peptides induced an equivalent level of targeted gene silencing but in most of the cases with lower cell toxicity as clearly shown in clonogenic assays.

scholarly journals The Three Essential Motifs in P0 for Suppression of RNA Silencing Activity of Potato leafroll virus Are Required for Virus Systemic Infection

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 170 ◽  
Author(s):  
Mamun-Or Rashid ◽  
Xiao-Yan Zhang ◽  
Ying Wang ◽  
Da-Wei Li ◽  
Jia-Lin Yu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Silencing ◽  
Rna Silencing ◽  
Systemic Infection ◽  
Rna Degradation ◽  
Potato Leafroll Virus ◽  
Posttranscriptional Gene Silencing ◽  
Conserved Region ◽  
Leafroll Virus ◽  
The Impact

Higher plants exploit posttranscriptional gene silencing as a defense mechanism against virus infection by the RNA degradation system. Plant RNA viruses suppress posttranscriptional gene silencing using their encoded proteins. Three important motifs (F-box-like motif, G139/W140/G141-like motif, and C-terminal conserved region) in P0 of Potato leafroll virus (PLRV) were reported to be essential for suppression of RNA silencing activity. In this study, Agrobacterium-mediated transient experiments were carried out to screen the available amino acid substitutions in the F-box-like motif and G139/W140/G141-like motif that abolished the RNA silencing suppression activity of P0, without disturbing the P1 amino acid sequence. Subsequently, four P0 defective mutants derived from a full-length cDNA clone of PLRV (L76F and W87R substitutions in the F-box-like motif, G139RRR substitution in the G139/W140/G141-like motif, and F220R substitution in the C-terminal conserved region) were successfully generated by reverse PCR and used to investigate the impact of these substitutions on PLRV infectivity. The RT-PCR and western blot analysis revealed that these defective mutants affected virus accumulation in inoculated leaves and systemic movement in Nicotiana benthamiana as well as in its natural hosts, potato and black nightshade. These results further demonstrate that the RNA silencing suppressor of PLRV is required for PLRV accumulation and systemic infection.

A synthetic amino acid substitution of Tyr10 in Aβ peptide sequence yields a dominant negative variant in amyloidogenesis

Aging Cell ◽  
2012 ◽  
Vol 11 (3) ◽  
pp. 530-541 ◽  
Author(s):  
Honoree Mazargui ◽  
Christian Lévêque ◽  
Dirk Bartnik ◽  
Jacques Fantini ◽  
Tiphany Gouget ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Dominant Negative ◽  
Peptide Sequence ◽  
Aβ Peptide ◽  
Synthetic Amino Acid ◽  
Dominant Negative Variant

1206 The effect of reduced crude protein, synthetic amino acid supplemented diets on nutrient excretion in wean to finish swine

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 579-579 ◽  
Author(s):  
C. E. Vonderohe ◽  
K. M. Mills ◽  
M. D. Asmus ◽  
E. R. Otto-Tice ◽  
J. Ni ◽  
...  
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Nutrient Excretion ◽  
Synthetic Amino Acid ◽  
Reduced Crude Protein
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